Experiments with the Chemostat on Spontaneous Mutations of Bacteria

Novick, Aaron; Szilard, Leo

doi:10.1073/pnas.36.12.708

Cited by 657 publications

(329 citation statements)

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“…The steady state is reached when the measured growth parameters (in this specific case metabolite consumption/production rate, biomass concentration and budding index) become constant. At steady state, the population is in exponential balanced growth, and the specific growth rate for each growing cell in the population [m5ln(2) (duplication time) 21 ] equals the dilution rate (Alberghina et al, 1998;Monod, 1950;Novick & Szilard, 1950).…”

Section: Resultsmentioning

confidence: 99%

Revisiting the role of yeast Sfp1 in ribosome biogenesis and cell size control: a chemostat study

et al. 2008

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Saccharomyces cerevisiae SFP1 promotes transcription of a large cluster of genes involved in ribosome biogenesis. During growth in shake flasks, a mutant deleted for SFP1 shows a small size phenotype and a reduced growth rate. We characterized the behaviour of an sfp1D mutant compared to an isogenic reference strain growing in chemostat cultures at the same specific growth rate. By studying glucose (anaerobic)-and ethanol (aerobic)-limited cultures we focused specifically on nutrient-dependent effects. Major differences in the genome-wide transcriptional profiles were observed during glucose-limited growth. In particular, Sfp1 appeared to be involved in the control of ribosome biogenesis but not of ribosomal protein gene expression. Flow cytometric analyses revealed size defects for the mutant under both growth conditions. Our results suggest that Sfp1 plays a role in transcriptional and cell size control, operating at two different levels of the regulatory network linking growth, metabolism and cell size.

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Section: Resultsmentioning

confidence: 99%

Revisiting the role of yeast Sfp1 in ribosome biogenesis and cell size control: a chemostat study

et al. 2008

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“…For example in the apical meristems, the population of apical initials is in continuous growth phase (at least during the growing season), the excess cells formed are "washed out" to the soma, and finally there is a continuous flow of nutrient medium (water, minerals, photosynthate) into the meristem. Novick andSzilard (1950, 1951) studied the dynamics of spontaneous mutation in microbial chemostats. The general principles that they developed can be applied to multicellular plants to give an appreciation of the impact of continued somatic mutation during plant growth.…”

Section: Plants As Chemostatsmentioning

confidence: 99%

Progressive cross- and self-sterility associated with aging in fern clones and perhaps other plants

Klekowski

1988

Heredity

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Plant reproductive cells differentiate from cell lineages derived ultimately from apical meristem initials, cells that are mitotically active throughout the life span of the plant. Because of the chemostat-like properties of these initial-cell populations, the frequency of mutant initials (or apical meristems) will increase as the plant ages. These properties led to three predictions when comparing long-lived species in which the frequency of sexual reproduction varies. The most asexual species should have the highest mean frequency of mutant ramets per chimeric genet, the highest genetic load, and the highest level of genetic load with dominant effects in embryonic and juvenile growth phases. These predictions were confirmed in a study of two fern species.

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“…Also, chemostat models play a key role for experimentally reproducing and understanding the behaviours of interacting organisms in lakes and wastewater treatment plants. The basic mathematical model for the chemostat was first derived in [40,41]. See [9,10,[18][19][20]28,39,46] for more recent discussions on the chemostat and its role in microbial ecology.…”

Section: Introductionmentioning

confidence: 99%

Stability and stabilization for models of chemostats with multiple limiting substrates

Mazenc

Malisoff

2012

Journal of Biological Dynamics

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We study chemostat models in which multiple species compete for two or more limiting nutrients. First, we consider the case where the nutrient flow and species removal rates and input nutrient concentrations are all given as positive constants. In that case, we use Brouwer degree theory to give conditions guaranteeing that the models admit globally asymptotically stable componentwise positive equilibrium points, from all componentwise positive initial states. Then we use the results to develop stabilization theory for a class of controlled chemostats with two or more limiting nutrients. For cases where the dilution rate and input nutrient concentrations can be selected as controls, we prove that many different componentwise positive equilibria can be made globally asymptotically stable. This extends the existing control results for chemostats with one limiting nutrient. We demonstrate our methods in simulations.

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Experiments with the Chemostat on Spontaneous Mutations of Bacteria

Cited by 657 publications

References 0 publications

Revisiting the role of yeast Sfp1 in ribosome biogenesis and cell size control: a chemostat study

Revisiting the role of yeast Sfp1 in ribosome biogenesis and cell size control: a chemostat study

Progressive cross- and self-sterility associated with aging in fern clones and perhaps other plants

Stability and stabilization for models of chemostats with multiple limiting substrates

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