Experiments with automated protein crystallization

Cox, M. J.; Weber, Patricia C

doi:10.1107/s0021889887086461

Cited by 45 publications

(20 citation statements)

References 12 publications

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“…In practice, crystallization conditions for proteins, viruses and nucleic acids are sought nowadays mainly by a trial and error approach involving thousands of experiments [10][11][12][13]. By applying the fundamentals of non-equilibrium crystallization to large macromolecules this time consuming search can be minimized.…”

Section: Fundaments and Motivation Of The Workmentioning

confidence: 99%

A supersaturation wave of protein crystallization

García-Ruiz

Otálora

Novella

et al. 2001

Journal of Crystal Growth

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A microgravity protein crystallization experiment is described in which the existence of a supersaturation wave traveling across a diffusion-reaction system is experimentally demonstrated for the first time. The self-organized dynamics of the experimental setup were used to implement a crystallization technique able to search automatically through the crystallization parameter space for optimum nucleation and growth conditions. The crystals obtained by this automatic optimization produced the highest quality X-ray diffraction data ever collected from the model protein used in the experiment. #

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Section: Fundaments and Motivation Of The Workmentioning

confidence: 99%

A supersaturation wave of protein crystallization

García-Ruiz

Otálora

Novella

et al. 2001

Journal of Crystal Growth

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“…Cox & Weber, 1987;Ward, Perozzo & Zuk, 1988;Chayen, Shaw Stewart, Maeder & Blow, 1990;Rubin, Talatous & Larson, 1991;Oldfield, Ceska & Brady, 1991;Chayen, Shaw Stewart & Blow, 1992;Soriano & FontecillaCamps, 1993;Sadaoui, Janin & Lewit-Bently, 1994;Chayen, Shaw Stewart & Baldock, 1994). The fundamental difference between the vapourdiffusion and microbatch methods is that diffusion methods are dynamic systems in which conditions are changing throughout the crystallization process and, hence, there is little control over the experiments once trials have been initiated.…”

Section: Comparison Of the Vapour-diffusion And Microbatch Methodsmentioning

confidence: 99%

Comparative Studies of Protein Crystallization by Vapour-Diffusion and Microbatch Techniques

Chayen

1998

Acta Crystallogr D Biol Crystallogr

105

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IntroductionNumerous reports have been published in the literature which describe the crystallization of macromolecules by a variety of crystallization methods, including the vapour-diffusion and microbatch techniques. This topical review compares the results of examples of proteins which were crystallized by both vapour-diffusion and microbatch methods. The inherent features of the vapourdiffusion and microbatch methods are discussed and some specific conditions where one method appears more favourable than the other are reported. Guidelines for the conversion of crystallization conditions from vapour diffusion to microbatch (and vice versa) are also presented.

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“…Micro-bridges have been used in combination with the 'Robotic Protein Crystallization System' from ICN Biomedicals (Cox & Weber, 1987). Up to now the robot has only been used with the bridges in a semi-automatic way, in which the robot pipettes the reservoir solutions and the precious protein droplets are pipetted by hand.…”

Section: Practical Experience With Micro-bridgesmentioning

confidence: 99%

Micro-bridges for sitting-drop crystallizations

Harlos¹

1992

J Appl Cryst

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A printer's error is corrected. In the paper by Reid & Clackson [J. Appl. Cryst. (1992), 25, 244-250], equation (A10) on page 249 should readAll relevant information is given in the Abstract. J. Appl. Cryst. (1992 Micro-bridges, small devices in the shape of a bridge, have been designed to carry out sitting-drop crystallizations on a microscale. Micro-bridges have an indentation in the top of the bridge to contain the protein drop during a crystallization experiment and to prevent the droplet from spreading over a large area. The special feature of micro-bridges is that they fit into the wells of standard tissue-culture multiwell plates.

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Experiments with automated protein crystallization

Cited by 45 publications

References 12 publications

A supersaturation wave of protein crystallization

A supersaturation wave of protein crystallization

Comparative Studies of Protein Crystallization by Vapour-Diffusion and Microbatch Techniques

Micro-bridges for sitting-drop crystallizations

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