Experiments of the fate of embryonic cells (chick) disseminated by the vascular route

Weiß, Paul; Andres, G.

doi:10.1002/jez.1401210303

Cited by 104 publications

(16 citation statements)

References 22 publications

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“…Andres (166) has injected dissociated cells from particular chick embryos into the circulation of other embryos and reports that teratomas of various composition are formed by the donor cells in the host embryonic membranes. These and earlier observations by Weiss & Andres (167) suggest that donor cells also participate in a type-specific localization within the embryonic host and are incorporated into its normal differentiation.…”

Section: Cellular Antigenssupporting

confidence: 75%

Physiological Aspects of Genetics

Horowitz¹,

Owen²

1954

Annu. Rev. Physiol.

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Section: Cellular Antigenssupporting

confidence: 75%

Physiological Aspects of Genetics

Horowitz¹,

Owen²

1954

Annu. Rev. Physiol.

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“…Although it is known that some factors such as mouse fibroblast cells (STO) as feeder cells, stem cell factor (SCF), leukemia inhibitory factor (LIF) and basic fibroblast growth factor (bFGF ) are necessary for the proliferation and survival of mouse PGCs in vitro [10], we know little P blast of the area pellucida [2]. The cells enter the circulating blood stream, where they are called circulating PGCs (cPGCs) [3], at the first heart beats [4] and migrate to the final destination, the gonad, where they are known as gonadal PGCs (gPGCs) [5], and undergo a process of proliferation and differentiation to become two kinds of gamete, ova and spermatozoa.…”

mentioning

confidence: 99%

Survival and Proliferation of Refined Chicken Circulating Primordial Germ Cells Cultured In Vitro

Yang

Fujihara

1999

Journal of Reproduction and Development

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Abstract. In recent years, much attention has been paid to the manipulation of primordial germ cells (PGCs) to produce transgenic chicken. For this purpose, the in vitro proliferation of PGCs would make gene targeting possible, promoting the efficiency of transgenes. However, few researches have been carried out on this topic. In the present study, we tried to culture chicken PGCs outside the body. The PGCs were isolated from circulating blood of chicken embryos by the method of Ficoll density centrifugation, and labeled with PKH26 red fluorescent cell linker. The cells were refined by selection with a fine glass pipette and a micromanipulator in order to avoid the contamination of PGCs with red cells. The refined cells were then cultured for a given period. The PGCs proliferated when they were cultured on feeder cells derived from the germinal ridges of chicken embryos at stage 27. During a 5 day culture period, the highest rate of increased PGCs was about 51% for one of the two test groups. Although some kinds of growth factors were supplemented in the culture media, no synergistic effect of chicken stem cell factor (chSCF) with murine leukemia inhibitory factor (LIF) and human basic fibroblast growth factor (bFGF) on the proliferation of PGCs was found. This result implies that the LIF and bFGF influencing the proliferation of PGCs may not be conserved between mammals and birds.

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“…For the latter theory speaks the fact that melanoblasts or -cytes might be found in corium and subcutis and that cytologists state that these are cells "which for one reason or another have stopped on their way out from the neural crest to their permanent place". (3) It is generally assumed that melanomas might activate junctional naevi in other regions, which means that some ingredient or other, a factor X, is produced somewhere and distributed through the blood. This factor X might possibly activate the above mentioned "displaced" pigment cells and thus give rize to metastases, and this too might explain their apparent habit of appearing at the same places as the primary lesions.…”

Section: Discussionmentioning

confidence: 99%

The Melanomas of the Skin and Their Cutaneous Metastases

Olsen¹

1959

Acta Radiologica

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Experiments of the fate of embryonic cells (chick) disseminated by the vascular route

Cited by 104 publications

References 22 publications

Physiological Aspects of Genetics

Physiological Aspects of Genetics

Survival and Proliferation of Refined Chicken Circulating Primordial Germ Cells Cultured In Vitro

The Melanomas of the Skin and Their Cutaneous Metastases

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