Experiments and theoretical calculations for forming gradients for zonal rotor centrifugation

“…The data presented are discussed with respect to the conformation transfer over 60 A from the anticodon loop to the T-$-C-G region. [6] and No11 et al [7]. Storage and reactivation of ribosomes prior to use were as described earlier [ 1 ] .…”

Section: Introductionmentioning

confidence: 99%

Codon‐dependent rearrangement of the tertiary structure of tRNA^Phe from yeast

Schwarz¹,

Gassen²

1977

FEBS Letters

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“…50S and 30S ribosomal subunits were prepared by zonal centrifugation (Leifer and Kreuzer, 1971). The ribosomal subunits were dissolved in 50 mM Tris-Cl, pH 7.5, 20 mM Mg(OAc)2i 200 mM NH4CI, and 2 mM 1,4-dithioerythritol to a final concentration of 160 A 250 units/ml.…”

mentioning

confidence: 99%

Codon-dependent rearrangement of the three-dimensional structure of phenylalanine tRNA, exposing the T-ψ-C-G sequence for binding to the 50S ribosomal subunit

Schwarz¹,

Menzel²,

Gassen³

1976

Biochemistry

123

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Codon-anticodon interaction induces an allosteric rearrangement of the three-dimensional structure of Phe-tRNAPhe that exposes the T-psi-C-G sequence for binding to the C-G-A-A sequence of the 5S rRNA within the 50S ribosomal subunit. The conformational change in the tRNAPhe structure was followed by the binding of C-G-[3H]A-[3H]A to the T-psi-C-G sequence, as measured by equilibrium dialysis at 10 mM Mg2+. C-G-A-A (14 pmol) was bound to tRNAPhe in the complete system containing elongation factor Tu-GTP-Phe-tRNA-(uridylyl-3',5')7-uridine-30S ribosomes (100 pmol). At a Mg2+ concentration lower than 5 mM the rearrangement was dependent on elongation factor-Tu, whereas GTP could be replaced by guanylyl imidodiphosphonate. In the absence of elongation factor-Tu-GTP a sigmoidal C-G-A-A binding curve with respect to Mg2+ concentration was obtained, showing half-saturation at 6 mM Mg2+. To achieve the change in the tRNAPhe structure in the absence of 30S ribosomes, a twofold higher concentration of (uridylyl-3',5')7-uridine had to be used. A sigmoidal curve was obtained again when the Mg2+ dependence of the C-G-A-A binding was followed, with 12 pmol of C-G-A-A being bound to 200 pmol of Phe-tRNA. Since T-psi-C-G exposure should influence the binding of Phe-tRNA to 70S ribosomes, Phe-tRNA binding to 70S ribosomes was examined. In the "nonenzymatic" binding (i.e., no elongation factor-Tu-GTP) of Phe-tRNA a sigmoidal Mg2+ dependence was found, whereas the "enzymatic" binding (elongation factor-Tu-GTP present) showed a hyperbolic curve. With 30S ribosomes as controls, only hyperbolic binding curves were found. The Mg2+ dependence of AA-tRNA binding thus reflects the rearrangement of the tRNA structure.

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Experiments and theoretical calculations for forming gradients for zonal rotor centrifugation

Cited by 15 publications

References 8 publications

Initiator‐tRNA recognizes a tetranucleotide codon during the 30 S initiation complex formation

Initiator‐tRNA recognizes a tetranucleotide codon during the 30 S initiation complex formation

Codon‐dependent rearrangement of the tertiary structure of tRNA^Phe from yeast

Codon-dependent rearrangement of the three-dimensional structure of phenylalanine tRNA, exposing the T-ψ-C-G sequence for binding to the 50S ribosomal subunit

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Experiments and theoretical calculations for forming gradients for zonal rotor centrifugation

Cited by 15 publications

References 8 publications

Initiator‐tRNA recognizes a tetranucleotide codon during the 30 S initiation complex formation

Initiator‐tRNA recognizes a tetranucleotide codon during the 30 S initiation complex formation

Codon‐dependent rearrangement of the tertiary structure of tRNAPhe from yeast

Codon-dependent rearrangement of the three-dimensional structure of phenylalanine tRNA, exposing the T-ψ-C-G sequence for binding to the 50S ribosomal subunit

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Codon‐dependent rearrangement of the tertiary structure of tRNA^Phe from yeast