Experimentelle Xenotransplantation in entfernt stammesverwandten Speciessystemen

Abstract

E vivo hemoperfusion of pig kidneys by untreated dogs results in a complete deterioration of function within 15 min caused by a hyperaeute xenogeneic rejection. Flush out of these kidneys by a special perfusion solution of 4°C is already able to delay the complete stop of the renal blood flow to approximately 2 hrs.Preformed natural antibodies together with complement, platelets, white blood cells and vasoactive substances are discussed to be the reason for this type of graft rejection.Extreme isovolemic hemodilution of the recipient dog with 6~o Dextran 60 to a hematocrit of 7.5~o eliminates most of the preformed natural antibodies. In parallel, all other factors like complement, 7 globulins, thrombocytes and leucocytes are significantly decreased.The wash out of these factors by gradually replacing whole blood with only Dextran 60 and washed autologous erythrocytes leads to a striking mitigation of the xcnogeneic reaction, resulting in a prolongation of the functional survival as indicated by extending the renal blood flow up to 11 hrs and a tremendous increase of urine production.