Experimentelle Untersuchungen zur Pathogenese des mikrobiellen Ekzems

The development of delayed hypersensitivity against staphylococcal antigens in guinea pigs was observed from day 3 to day 50 after sensitization with staphylococcal homogenate in Freund's incomplete (FIA) and Freund's complete adjuvant (FCA). Skin test reactivity, stimulation of lymph node lymphocytes, and peripheral blood lymphocytes and the titre of precipitating antibodies were followed during this period. Maximal skin test reactivity as well as maximal lymphocyte responsiveness occurred at day 21 after sensitization in FCA-sensitized guinea pigs. In FIA-sensitized animals highest skin reactivity was observed at day 14 and maximal lymphocyte stimulation 35 days after sensitization. Precipitating antibodies reached a plateau at day 20 in plasma of animals sensitized with FCA and at day 35 in FIA-sensitized animals.

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Skin test and lymphocyte stimulation in delayed hypersensitivity against staphylococcal antigens

Weiss

et al. 1980

Arch Dermatol Res

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Staphylococcal homogenate was fractionated into cell walls (CW), cell membranes (CM) (insoluble part left after removal of the cell wall fraction) and a soluble fraction. The capacity of these fractions to evoke delayed skin reactions and to stimulate lymph node and peripheral blood lymphocytes from guinea pigs sensitized with staphylococcal homogenate in Freund's complete adjuvant was tested 21 days after sensitization. Highest skin reactivity was observed with the cell wall fraction. In the lymphocyte stimulation test similar results were obtained with all three fractions. With peripheral blood lymphocytes higher stimulation indices were observed than with lymph node lymphocytes.

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Experimentelle Untersuchungen zur Pathogenese des mikrobiellen Ekzems

Cited by 17 publications

References 21 publications

Experimental Investigations on the Acantholysis Induced by Staphylococcus pyogens. Comparative Study with the Acantholysis on Pemphigus

Experimental Investigations on the Acantholysis Induced by Staphylococcus pyogens. Comparative Study with the Acantholysis on Pemphigus

Skin test and lymphocyte stimulation in delayed hypersensitivity against staphylococcus aureus antigens

Skin test and lymphocyte stimulation in delayed hypersensitivity against staphylococcal antigens

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