Experimentelle Untersuchungen �ber Beziehungen zwischen Bakteriophagen und malignen Tumoren

In the past years, the microbiome and its role in the pathophysiology of diseases have gained great interest. The progress of our knowledge in this field opens completely novel prospects for treating disorders, including those which are most challenging to medicine today. Of special interest are studies on the interactions of the microbiome with the immune system. Only recently has the presence of bacteriophages in the microbiome been highlighted, and their potential role in maintaining normal immunity has gained increasing attention. We summarize the available data pointing to the potential impact of phages in maintaining immunological homeostasis.

show abstract

“…This confirms observations made already in 1940 indicating that phages have antitumor activity in mice and rabbits [64].…”

Section: Anticancer Effects Of Phagessupporting

confidence: 80%

Phages and Immunomodulation

et al. 2017

View full text Add to dashboard Cite

show abstract

“…In this implementation, tumor-specific delivery is achieved via the EPR effect. The EPR effect is the spontaneous accumulation of nanoparticles in tumors, observed for an uncharacterized phage in 1940 [18] (reviewed in [19]). The causes of the EPR effect are (1) porosity of tumor blood vessels and relative tightness of healthy blood vessels, so that nanoparticles enter tumors, but usually not healthy tissue and (2) poor tumor lymphatic drainage, so that nanoparticles remain [20][21][22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Phage Capsids as Gated, Long-Persistence, Uniform Drug Delivery Vehicles

Serwer¹,

Wright²,

Gonzales³

2020

Current and Future Aspects of Nanomedicine

View full text Add to dashboard Cite

Over the last 25 years, cancer therapies have improved survivorship. Yet, metastatic cancers remain deadly. Therapies are limited by inadequate targeting. Our goal is to develop a new drug delivery vehicle (DDV)-based strategy that improves targeting of drug delivery to solid tumors. We begin with a capsid nanoparticle derived from bacteriophage (phage) T3, a phage that naturally has high persistence in murine blood. This capsid has gating capacity. For rapidly detecting loading in this capsid, here, we describe procedures of native agarose gel electrophoresis, coupled with fluorescence-based detection of loaded molecules. We observe the loading of two fluorescent compounds: the dye, GelStar, and the anticancer drug, bleomycin. The optimal emission filters were found to be orange and green, respectively. The results constitute a first milestone in developing a drug-loaded DDV that does not leak when in blood, but unloads its cargo when in a tumor.

show abstract