Experimentally Induced Unilateral Tooth Loss: Histochemical Studies of                the Temporomandibular Joint

Huang, Qihua; Opstelten, Davina; Samman, N; Tideman, H.

doi:10.1177/0810209

Cited by 45 publications

(33 citation statements)

References 16 publications

(23 reference statements)

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“…This controversy exists because an integrative biomechanical, cellular and biochemical analysis of adaptive plasticity heretofore had been applied only to cranial synovial joints (TMJ) (Bouvier and Hylander, 1982;Bouvier and Hylander, 1984;Huang et al, 2002;Huang et al, 2003) and syndesmoses (sutures) (Byron et al, 2004). To this end, data on tissue plasticity for a cranial arthrosis (rabbit symphysis) offer a novel perspective on the dynamic inter-relationships among symphyseal fusion, joint performance and feeding behaviors.…”

Section: Adaptive Plasticity and Symphyseal Functionmentioning

confidence: 99%

“…Increased alkaline phosphatase activity associated with biomineralization of TMJ condylar tissues, and changes in osteoclastic and osteoblastic activity also have been noted (Bouvier, 1988;Kim et al, 2003). In addition, altering TMJ force application by varying masticatory loading regime, tooth extraction, unilateral bite raise or corticotomy has been shown to result in gene expression changes and elevated glycosaminoglycan (GAG) content in condylar cartilage (Copray et al, 1985;Carvalho et al, 1995;Holmvall et al, 1995;Pirttiniemi et al, 1996;Mao et al, 1998;Agarwal et al, 2001;Huang et al, 2002;Huang et al, 2003). Lastly, changes in expression of type I and type II collagen vary in response to joint loads, further supporting the hypothesis that mechanotransduction signals changes in gene expression that alter tissue proliferation, composition and function as a response to induced degeneration of the cartilage matrix (Mizoguchi et al, 1996;Pirttiniemi et al, 1996;Grodzinsky et al, 2000;Honda et al, 2000;Lee et al, 2000;Huang et al, 2003;Kim et al, 2003;Wong and Carter, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…Histological and immunohistochemical analysis of symphyseal and TMJ tissues followed standard procedures (Scapino, 1981;Trevisan and Scapino, 1976a;Trevisan and Scapino, 1976b;Beecher, 1977;Beecher, 1979;Hirschfeld et al, 1977;Bouvier and Hylander, 1982;Bouvier and Hylander, 1984;Bouvier, 1987;Bouvier, 1988;Kiernan, 1999;Huang et al, 2002;Kim et al, 2003;Ravosa and Hogue, 2004). Joints were fixed in 10% neutral buffered formalin.…”

Section: Histology and Immunohistochemistry Of Cartilage Compositionmentioning

confidence: 99%

“…The cationic dye Safranin O was used to evaluate relative GAG content in the symphyseal fibrocartilaginous pad and TMJ articular/hyaline cartilage (Kiernan, 1999;Huang et al, 2002). Primary antibodies directed at variation in cartilage type II collagen were employed to assess collagen and proteoglycan relative expression pattern (i.e.…”

Section: Histology and Immunohistochemistry Of Cartilage Compositionmentioning

confidence: 99%

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Pushing the limit: masticatory stress and adaptive plasticity in mammalian craniomandibular joints

Ravosa

Kunwar

Stock

et al. 2007

Journal of Experimental Biology

149

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SUMMARY Excessive, repetitive and altered loading have been implicated in the initiation of a series of soft- and hard-tissue responses or `functional adaptations' of masticatory and locomotor elements. Such adaptive plasticity in tissue types appears designed to maintain a sufficient safety factor, and thus the integrity of given element or system, for a predominant loading environment(s). Employing a mammalian species for which considerable in vivo data on masticatory behaviors are available, genetically similar domestic white rabbits were raised on diets of different mechanical properties so as to develop an experimental model of joint function in a normal range of physiological loads. These integrative experiments are used to unravel the dynamic inter-relationships among mechanical loading, tissue adaptive plasticity, norms of reaction and performance in two cranial joint systems:the mandibular symphysis and temporomandibular joint (TMJ). Here, we argue that a critical component of current and future research on adaptive plasticity in the skull, and especially cranial joints, should employ a multifaceted characterization of a functional system, one that incorporates data on myriad tissues so as to evaluate the role of altered load versus differential tissue response on the anatomical, cellular and molecular processes that contribute to the strength of such composite structures. Our study also suggests that the short-term duration of earlier analyses of cranial joint tissues may offer a limited notion of the complex process of developmental plasticity, especially as it relates to the effects of long-term variation in mechanical loads, when a joint is increasingly characterized by adaptive and degradative changes in tissue structure and composition. Indeed, it is likely that a component of the adaptive increases in rabbit TMJ and symphyseal proportions and biomineralization represent a compensatory mechanism to cartilage degradation that serves to maintain the overall functional integrity of each joint system. Therefore, while variation in cranial joint anatomy and performance among sister taxa is, in part, an epiphenomenon of interspecific differences in diet-induced masticatory stresses characterizing the individual ontogenies of the members of a species,this behavioral signal may be increasingly mitigated in over-loaded and perhaps older organisms by the interplay between adaptive and degradative tissue responses.

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Section: Adaptive Plasticity and Symphyseal Functionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Histology and Immunohistochemistry Of Cartilage Compositionmentioning

confidence: 99%

Section: Histology and Immunohistochemistry Of Cartilage Compositionmentioning

confidence: 99%

See 2 more Smart Citations

Pushing the limit: masticatory stress and adaptive plasticity in mammalian craniomandibular joints

Ravosa

Kunwar

Stock

et al. 2007

Journal of Experimental Biology

149

View full text Add to dashboard Cite

show abstract

“…The binding intensity of the safranin O stain was correlated to the GAG fraction similarly to Huang et al, 2002. 26 In short, the intensities of the red (R), green (G), and blue (B) channels were assigned values from 0 to 255. The proportion of red color to all of the colors primarily represented the safranin O binding and was calculated using the equation r = R/(R 2 + G 2 + B 2 ) 1/2 .…”

Section: Gag Quantificationmentioning

confidence: 99%

A Quantitative Study of the Microstructure and Biochemistry of the Medial Meniscal Horn Attachments

Villegas

Hansen

Liu³

et al. 2007

Ann Biomed Eng

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Abstract-Little quantitative data is available on the structure of meniscal attachments. Therefore, as an aid to designing meniscal replacements as well as a possible explanation for mechanical behavior, this study was designed to further the knowledge of the microstructure and biochemistry of native meniscal attachments. Bovine medial meniscal attachments (the external ligamentous portion as well as the transition zones at the bony insertion) were removed and prepared for microstructural evaluation. After embedding in paraffin, the samples were sliced on a microtome and stained for quantitative analysis. The anterior and posterior insertion sites are known to contain three zones: subchondral bone, calcified fibrocartilage, and uncalcified fibrocartilage. Additionally, others have shown that the anterior insertion site contains a ligamentous zone. The insertion zones were further divided into proximal, middle, and distal zones. The posterior attachment's insertion site had a significantly greater thickness of interdigitations, subchondral bone, uncalcified fibrocartilage, and calcified fibrocartilage zone thickness compared to the anterior attachment insertion. The anterior attachment's insertion had the greatest GAG fraction in each zone when compared to the posterior attachment's insertion. GAG fraction decreased from the meniscus to the subchondral bone. Both GAG fraction and normalized thickness varied within a given zone, decreasing from the distal to proximal regions in both the anterior and posterior attachments' insertion zones. Crimp frequency of the collagen fibrils in the external ligamentous portion of the tissue was homogeneous along the length. The findings from this study agree with previously published material property data on the medial meniscal attachments, and could be used in the future to design methods of attachment for tissue engineered replacement menisci.

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Unilateral Loss of Maxillary Molars in Young Mice Leads to Bilateral Condylar Adaptation and Degenerative Disease

Chen

Zhang

et al. 2022

JBMR Plus

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The adaptive response of the mandible and temporomandibular joint (TMJ) to altered occlusion in juvenile patients is presently unclear. To address this question, we established a mouse model in which all molars were extracted from the maxillary right quadrant in prepubertal, 3-week-old mice and analyzed morphological, tissue, cellular, and molecular changes in the mandible and condyle 3 weeks later. Unilateral loss of maxillary molars led to significant, robust, bilateral changes, primarily in condylar morphology, including anteroposterior narrowing of the condylar head and neck and increased convexity at the condylar surface, as determined by geometric morphometric analysis. Furthermore, both condyles in experimental mice exhibited a degenerative phenotype, which included decreased bone volume and increased mineral density near the condylar head surface compared to control mice. Changes in condylar morphology and mineralized tissue composition were associated with alterations in the cellular architecture of the mandibular condylar cartilage, including increased expression of markers for mature (Col2a1) and hypertrophic (Col10a1) chondrocytes, suggesting a shift toward differentiating chondrocytes. Our results show significant bilateral condylar morphological changes, alterations in tissue composition, cellular organization, and molecular expression, as well as degenerative disease, in response to the unilateral loss of teeth. Our study provides a relatively simple, tractable mouse tooth extraction system that will be of utility in uncovering the cellular and molecular mechanisms of condylar and mandibular adaptation in response to altered occlusion.

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Experimentally Induced Unilateral Tooth Loss: Histochemical Studies of the Temporomandibular Joint

Cited by 45 publications

References 16 publications

Pushing the limit: masticatory stress and adaptive plasticity in mammalian craniomandibular joints

Pushing the limit: masticatory stress and adaptive plasticity in mammalian craniomandibular joints

A Quantitative Study of the Microstructure and Biochemistry of the Medial Meniscal Horn Attachments

Unilateral Loss of Maxillary Molars in Young Mice Leads to Bilateral Condylar Adaptation and Degenerative Disease

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