Experimental validation of eosin-mediated photo-redox polymerization mechanism and implications for signal amplification applications

Abstract

When eosin-mediated, photo-redox polymerization is used to amplify signals in biosensing, oxygen has dual, opposing roles.

Electrochemical Detection of Femtomolar DNA via Boronate Affinity-Mediated Decoration of Polysaccharides with Electroactive Tags

“…As covalently bonded materials composed of many repeating subunits, polymers, both synthetic and natural, have found long-lasting application in the amplified detection of biological targets, such as nucleic acids, , proteins or enzymes, , and glycoproteins. , For example, the de novo synthesis of polymers through radical polymerization, − like reversible addition–fragmentation chain-transfer (RAFT) polymerization, eosin-mediated photo-redox polymerization, , and atom transfer radical polymerization (ATRP), , has been explored as an approach to recruiting numerous signal tags (e.g., phenolphthalein and ferrocene (Fc)) for amplified detection. For example, the de novo synthesis of ferrocenyl polymers through the biologically mediated RAFT polymerization (BMRP) of Fc-bearing monomers has been explored by us for the amplified electrochemical detection of kinase activity .…”