Experimental Validation and Simulation of Fourier and Non-Fourier Heat Transfer Equation during Laser Nano-Phototherapy of Lung Cancer Cells: An <i>in Vitro</i> Assay

Abstract

This paper investigated the numerical scheme extended to solve the hyperbolic non-Fourier form of bioheat transfer equation and the experimental trials were conducted to validate the numerical simulation. MNPs were prepared via co-precipitation and modified with a silica layer. The amino modified Fe 3 O 4 /SiO 2 nanoshells were covered with gold colloids producing nanoshells of Fe 3 O 4 /SiO 2 /Au (MNSs). In vitro assays were performed to determine the effect of apoptosis of QU-DB lung cancer cells based on the cells morphology changes. Cell damage was reduced by decreasing the power density of laser. Also, a larger area of damage on cell culture plates was observed at longer intervals of laser irradiation. The effect of nanoshell concentration and irradiation rate has been evaluated. A maximum temperature rise of 6˚C was achieved at 184 W/cm 2 and concentration of 0.01 mg/ml. The experiment confirmed a hyperbolic behaviour of thermal propagation. The results revealed that the three-dimensional implementation of bioheat equation is likely to be more accurate than the two-dimensional study.

Laser Auto Fluorescence and Dye-enhanced Spectroscopic Detection of Normal and Cancerous Human Lung Cell Lines and Plasmonic Photothermal Heating

“…The conventional treatments of cancers include surgical resection, chemotherapy, radiation therapy and targeted drug therapy but the advancement in engineered nanostructures during last decade has made the bioimaging, drug delivery and therapy significantly advantageous [13][14][15][16][17][18].…”

Dual Mode Atomic Force Microscopy and Interferometric Scattering Imaging of Single Au@Fe3O4 Nanoshell Synthesized for Biomedical Applications

“…Recently, the influence of nanoparticle aggregation on the efficiency of electromagnetic absorption has been studied in order to lower the threshold fluence level for biomedical applications such as laser-induced photothermal therapy [3]. From photothermal therapy perspective, a wavelength with maximal absorption and high absorption cross-section are the key features to consider [7][8][9][10][11]. However, when studying single nanoparticles, it is crucial to verify that one does not detect aggregation.…”