Experimental transmission, pathogenicity and physical–chemical properties of infectious spleen and kidney necrosis virus (ISKNV)

He, Jian; Zeng, Ke; Weng, Shaoping; Chan, Siu-Ming

doi:10.1016/s0044-8486(01)00639-1

Cited by 144 publications

(122 citation statements)

References 13 publications

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“…It has been reported that mandarinfish Siniperca chuatsi is the only species affected in natural outbreaks of the ISKNV (He et al 2002). However, iridoviral DNA sequences, MCIV, were detected in paraffin-embedded tissues from Murray cod Maccullochella peelii peelii that died during epizootics in Australia .…”

Section: Discussionmentioning

confidence: 99%

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Outbreaks and risks of infectious spleen and kidney necrosis virus disease in freshwater ornamental fishes

Jeong¹,

Kim²,

Jun³

et al. 2008

Dis. Aquat. Org.

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We examined the distribution of iridoviruses in 10 freshwater ornamental fish species hatched in Korea and imported from other Asian countries using both 1-step and 2-step polymerase chain reation (PCR). None of the 10 fish species analyzed were free of iridovirus as shown by 2-step PCR positive results, and 3 species yielded 1-step PCR positive results with associated mortality. Cloned PCR amplicons of the adenosine triphosphatase (ATPase) and major capsid protein (MCP) genes in genomic DNA of iridovirus showed the same nucleotide sequences as that of infectious spleen and kidney necrosis virus (ISKNV) isolated from the mandarinfish Siniperca chuatsi. These results indicate the presence of ISKNV disease in various ornamental fish as new host species and that the disease is widespread throughout different Asian countries including Korea, Singapore and China. Such infections were either clinical with associated mortality (and 1-step PCR positive) or asymptomatic in fish that were externally healthy (and only positive in 2-step PCR). Molecular analyses of the K2 region performed on iridovirus samples isolated from freshwater ornamental fishes revealed deletion/insertion of repetitive sequences of various lengths (42 to 339 bp), depending on the ISKNV isolates, without substitutions. Experimental infection of pearl gourami Trichogaster leeri and silver gourami T. microlepis with a tissue homogenate of pearl gourami infected by ISKNV induced 70 and 20% cumulative mortalities in the pearl and silver gourami, respectively. KEY WORDS: Iridovirus · ISKNV · Ornamental fish · Asymptomatic infection · Asian countries Resale or republication not permitted without written consent of the publisherDis Aquat Org 78: [209][210][211][212][213][214][215] 2008 (ALIV) and dwarf gourami C. lalia iridovirus (DGIV) in Japan.Infectious spleen and kidney necrosis virus (ISKNV), recently assigned to the new Megalocytivirus genus of the Iridoviridae family, based on morphological and genetic characteristics (Chinchar et al. 2005), has caused significant economic loss in the mandarinfish Siniperca chuatsi aquaculture sector of China (He et al. 2000). In studies to elucidate the transmission and host range of ISKNV, mandarinfish appears to be the only species infected naturally and the most susceptible species, followed by largemouth bass in experimental infections of 21 fish species tested (He et al. 2002). In addition, the presence of ISKNV has only been reported in China. Jeong et al. (2006a), using 2-step polymerase chain reation (PCR), reported megalocytivirus in marine fish species that were externally healthy, a condition that could be called persistent or asymptomatic infection. Moreover, investigation of largemouth bass Micropterus salmoides salmoides, using a 1-step PCR technique after the occurrence of mass mortality, has also revealed the long-term persistence of ranavirus, largemouth bass virus (LMBV), in the population (Hanson & Petrie-Hanson 2001).Although Megalocytivirus and Ranavirus are epidemiologically and g...

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Section: Discussionmentioning

confidence: 99%

“…In studies to elucidate the transmission and host range of ISKNV, mandarinfish appears to be the only species infected naturally and the most susceptible species, followed by largemouth bass in experimental infections of 21 fish species tested (He et al 2002). In addition, the presence of ISKNV has only been reported in China.…”

Section: Introductionmentioning

confidence: 99%

Outbreaks and risks of infectious spleen and kidney necrosis virus disease in freshwater ornamental fishes

Jeong¹,

Kim²,

Jun³

et al. 2008

Dis. Aquat. Org.

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“…However, outbreaks of diseases caused by parasites, bacteria and viruses have caused severe economic losses to the aquaculture industry, and in some cases the mortality caused by infectious spleen and kidney necrosis virus (ISKNV) can reach as high as 100% (He et al, 2002). Despite the economic importance of the fish and severe economic losses caused by diseases, little research has been carried out on the mandarin fish immune factors.…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning of the viperin gene and its promoter region from the mandarin fish Siniperca chuatsi

Sun

Nie

2004

Veterinary Immunology and Immunopathology

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“…Its annual yield in China is about 300,000 tons; Guangdong Province alone produces 100,000 tons. Recently, there has been a decline in the growth rate of pond-cultured S. chuatsi, a shortened period of sexual maturity, and reduced anti-disease ability, which seriously affect the quality and safety of the product (He et al, 2002). It is thought that this is due to germplasm degeneration, which results in decreased disease resilience in the offspring.…”

Section: Introductionmentioning

confidence: 99%

Simple sequence repeat-based analysis of the genetic diversity and population genetic structure of populations of Siniperca chuatsi

Sun¹,

Ye²,

Tian³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. In order to provide genetic information for the selective breeding of Siniperca chuatsi, 14 microsatellite DNA loci were used to evaluate the genetic diversity and structure of four farmed populations and one wild population in China. The four cultivated populations were Foshan (FS), Jiangmen (JM), Nanjing (NJ), and Hongze Lake (HZL), and the wild population was collected from the Hubei HuangGang section of the Yangtze River (HG). All five populations exhibited high genetic diversity (H E values of between 0.608 and 0.633); the highest was found in the wild population (H E = 0.633). Genetic differentiation within the populations was relatively low (F ST < 0.15); 5.44% of the genetic variation was between the populations and 94.56% was within the populations. The greatest genetic distance was between JM and HG (0.1894), which had the lowest genetic identity (0.8725). NJ and HG had the shortest genetic distance (0.0365) and the highest genetic identity (0.9641). A phylogenetic analysis revealed that FS, JM, and HZL were clustered into one group, while NJ and HG were in another group, suggesting that the wild and NJ populations were closely related. Our results demonstrate that although the farmed populations have maintained a relatively high genetic diversity, they exhibit lower genetic diversity and higher genetic differentiation than the wild population. These results provide evidence that wild resources should be used for breeding, in order to maintain genetic diversity and ensure sustainable S. chuatsi farming.

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Experimental transmission, pathogenicity and physical–chemical properties of infectious spleen and kidney necrosis virus (ISKNV)

Cited by 144 publications

References 13 publications

Outbreaks and risks of infectious spleen and kidney necrosis virus disease in freshwater ornamental fishes

Outbreaks and risks of infectious spleen and kidney necrosis virus disease in freshwater ornamental fishes

Molecular cloning of the viperin gene and its promoter region from the mandarin fish Siniperca chuatsi

Simple sequence repeat-based analysis of the genetic diversity and population genetic structure of populations of Siniperca chuatsi

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