Experimental transmission of Theileria uilenbergi infective for small ruminants by Haemaphysalis longicornis and Haemaphysalis qinghaiensis

Li, Youquan; Luo, Jianxun; Guan, Guiquan; Ma, Miling; Liu, Aihong; Liu, Junlong; Ren, Qiaoyun; Niu, Qingli; Lu, Bingyi; Gao, Jinliang; Liu, Zhijie; Dang, Zhisheng; Tian, Zhancheng; Bao, Zhenan; Zhang, He; Bai, Quan; Yin, Hong

doi:10.1007/s00436-009-1347-7

Cited by 49 publications

(25 citation statements)

References 17 publications

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“…Compared with T. uilenbergi and T. luwenshuni, the round forms and the oval forms almost exist in every stage of the disease and they seem to be the dominant morphology in the infection of T. ovis. However, the pyriform and the needle appeared first, and they seem to be the dominant morphology in the infection of T. uilenbergi and T. luwenshuni (Li et al 2007(Li et al , 2009). Uilenberg (1981) also reported that in both species (T. lestiquardi and T. ovis), the piroplasms are small and predominantly round or oval.…”

Section: Discussionmentioning

confidence: 96%

Experimental transmission of Theileria ovis by Hyalomma anatolicum anatolicum

Guan

Liu

et al. 2010

Parasitol Res

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The experimental transmission of a newly isolated Theileria ovis is described. Hyalomma anatolicum anatolicum nymphs developed from larvae engorged on sheep infected with T. ovis were able to transmit the T. ovis to splenectomized sheep. Meanwhile, H. anatolicum anatolicum adults developed from nymphs engorged on sheep infected with T. ovis were also able to transmit T. ovis to splenectomized sheep. These experiments suggested that T. ovis could be transmitted by a H. anatolicum anatolicum, and the mode of transmission is from stage to stage.

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Section: Discussionmentioning

confidence: 96%

Experimental transmission of Theileria ovis by Hyalomma anatolicum anatolicum

Guan

Liu

et al. 2010

Parasitol Res

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“…Piroplasma were transmissed with H. qinghaiensis to show in China previously Yin et al 2002a, b, c;Li et al 2007Li et al , 2009Guan et al 2010). In the present study, we determined prevalence of piroplasm infection (Theileria ?…”

Section: Discussionmentioning

confidence: 99%

Simultaneous detection of piroplasma infections in field Haemaphysalis qinghaiensis ticks by reverse line blotting

et al. 2011

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Piroplasmosis is caused by tick-borne haemoprotozoan parasites in the genera of Theileria and Babesia, in which numbers of agents are highly pathogenic for cattle, sheep and goats. We developed a reverse line blot (RLB) assay for detection and differentiation of four different parasites, in which 18S ribosomal RNA gene sequence was amplified with a set of universal primers specific for all members in the genera of Theileria and Babesia; and the probes were designed on the basis of hypervariable region 4 (V4 region) of 18S rRNA gene. Three Theileria and one Babesia can be detected simultaneously on this system and it was sensitive to detect a parasitemia level between 10(-5) and 10(-8)%. A total of 149 Haemaphysalis qinghaiensis ticks collected from Lintan County of Gannan Tibetan Autonomous Region was tested by RLB. Among these, 136 tick samples were also tested by a nested PCR assay developed previously. After comparison of these results, it showed that more T. luwenshuni was detected in RLB assay, while more T. uilenbergi was detected in H. qinghaiensis ticks by nested PCR. The RLB has shown capability for simultaneous detection of four species of piroplasm in H. qinghaiensis ticks, indicating its usefulness for epidemiological studies of piroplasmosis.

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“…and babesiosis caused by Babesia spp. in sheep and cattle (Guan et al 2002;Li et al 2009). These two ticks are mainly distributed in Pastoral lands located in Qinghai and Gansu provinces.…”

Section: Introductionmentioning

confidence: 99%

Discrimination between Haemaphysalis longicornis and H. qinghaiensis based on the partial 16S rDNA and the second internal transcribed spacer (ITS-2)

et al. 2011

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In the present study, two hard tick species, Haemaphysalis longicornis and H. qinghaiensis from North-western China were characterized genetically by the second internal transcribed spacer (ITS-2) of nuclear ribosomal DNA and partial 16S rDNA. Based on a fragment within the hypervariable region of 16S rDNA with the length of approximately 453 bp, the phylogenetic trees were constructed by Neighbor-Joining and Maximum-parsimony methods. The results indicated that the phylogenetic status of H. qinghaiensis was distant from that of H. longicornis and closer to H. flava. Furthermore, the ITS-2 rDNA was amplified by PCR and sequenced from individual ticks. The length of ITS-2 is 1,606 bp for H. longicornis and 1,162 bp for H. qinghaiensis. Although sequence variation between the immature stages of H. longicornis was 0.1-0.4%, nucleotide differences between the tested species ranged 2.1-23.2%, indicating that ITS-2 rDNA sequences are genetic markers for the differentiation of the two hard ticks in China. Hence, a PCR-linked restriction fragment length polymorphism (RFLP) approach was developed for their unequivocal differentiation based on ITS-2 rDNA, which provides the foundation for further studies on ticks in China and has implications for studying the population genetic structure of the ticks and for identification and differentiation of closely related ticks.

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Experimental transmission of Theileria uilenbergi infective for small ruminants by Haemaphysalis longicornis and Haemaphysalis qinghaiensis

Cited by 49 publications

References 17 publications

Experimental transmission of Theileria ovis by Hyalomma anatolicum anatolicum

Experimental transmission of Theileria ovis by Hyalomma anatolicum anatolicum

Simultaneous detection of piroplasma infections in field Haemaphysalis qinghaiensis ticks by reverse line blotting

Discrimination between Haemaphysalis longicornis and H. qinghaiensis based on the partial 16S rDNA and the second internal transcribed spacer (ITS-2)

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