Experimental tracheal replacement using tissue-engineered cartilage

Vacanti, Charles A.; Paige, Keith T.; Kim, Woo Seob; Sakata, Junichi; Upton, Joseph; Vacanti, Joseph P.

doi:10.1016/0022-3468(94)90318-2

Cited by 165 publications

(95 citation statements)

References 15 publications

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“…Vacanti et al observed no immune response to PGA in nude mice [137]; however, Kojima et al observed poor cartilage formation in the same PGA constructs in sheep after 7 days, which the authors concluded was likely due to an inflammatory response [136]. A similar immune response was observed in PLA/PGA constructs in rabbits [138].…”

Section: Synthetic and Biologically Derived Polymersmentioning

confidence: 93%

“…The polymers were mostly designed to be hydrolytically degraded in vivo into easily removed fragments. Materials used include polyglycolic acid (PGA) [135,136,137], polylactic acid and polyglycolic acid mixture (PLA/PGA) and poly(lacticcoglycolic acid) PLGA [138], polyester urethane [139], poly(ethylene oxide)-terephthalate/poly(butylene terephthalate)(PEOT/PBT) [140,141], and gelatin sponge [142].…”

Section: Synthetic and Biologically Derived Polymersmentioning

confidence: 99%

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Tracheal Tissue Engineering

Birla

2014

Introduction to Tissue Engineering

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Section: Synthetic and Biologically Derived Polymersmentioning

confidence: 93%

Section: Synthetic and Biologically Derived Polymersmentioning

confidence: 99%

Tracheal Tissue Engineering

Birla

2014

Introduction to Tissue Engineering

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“…[1][2][3][4][5] In recent years, considerable attention has been given to biodegradable synthetic polymers and their application in cartilage tissue engineering. 6,7 Scaffolds seeded with chondrocytes or stem cells have been widely investigated for use in the tissue engineering of cartilage.…”

Section: Introductionmentioning

confidence: 99%

Electrospun gelatin/polycaprolactone nanofibrous membranes combined with a coculture of bone marrow stromal cells and chondrocytes for cartilage engineering

Feng

Huang

et al. 2015

IJN

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Electrospinning has recently received considerable attention, showing notable potential as a novel method of scaffold fabrication for cartilage engineering. The aim of this study was to use a coculture strategy of chondrocytes combined with electrospun gelatin/polycaprolactone (GT/PCL) membranes, instead of pure chondrocytes, to evaluate the formation of cartilaginous tissue. We prepared the GT/PCL membranes, seeded bone marrow stromal cell (BMSC)/chondrocyte cocultures (75% BMSCs and 25% chondrocytes) in a sandwich model in vitro, and then implanted the constructs subcutaneously into nude mice for 12 weeks. Gross observation, histological and immunohistological evaluation, glycosaminoglycan analyses, Young’s modulus measurement, and immunofluorescence staining were performed postimplantation. We found that the coculture group formed mature cartilage-like tissue, with no statistically significant difference from the chondrocyte group, and labeled BMSCs could differentiate into chondrocyte-like cells under the chondrogenic niche of chondrocytes. This entire strategy indicates that GT/PCL membranes are also a suitable scaffold for stem cell-based cartilage engineering and may provide a potentially clinically feasible approach for cartilage repairs.

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“…However, this established method is not appropriate for the repair of extensive lesions. Therefore, for these types of lesions other replacement methods, such as autografts [2,3], allografts [4,5], prosthetics materials [6][7][8] and tissue engineering [9][10][11][12] have been studied and developed. Some partial successes have been announced with some of these methods, butso far not one of them has archived a completely satisfactory long-term tracheal replacement for extensive lesions of the trachea.…”

Section: Introductionmentioning

confidence: 99%

“…The last decade of the 20 th century saw an influx of newresearch concepts in terms of tissue engineering, which has had a revolutionary impact on the research of tracheal prosthesis [14]. In 1994 two of the first reports on the effort to engineer a trachea were made by Osada and colleagues [9] and Vacanti and colleagues [10]. With different approaches, both groups were trying to recreate the cylindrical shape of the trachea, combining material and cells to make a more biocompatible tracheal replacement.…”

Section: Introductionmentioning

confidence: 99%

Tissue Engineered Trachea Using Decellularized Aorta

Paz¹,

Kojima²,

Iwasaki³

2011

J Bioengineer & Biomedical Sci

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Background: Several approaches for the development of tracheal substitutes for the treatment of extensive tissue defects have been explored over the years. However, a completely satisfactory approach has not been achieved. Previously, we described a composite tissue engineered trachea (TET) using chondrocytes seeded onto a polyglycolic acid (PGA) fiber-mesh. This study was considered to improve the design and functionality of the TET by using a porcine decellularized aorta as the scaffold.Methods: Chondrocytes were harvested from sheep tracheal cartilage and were suspended in medium. The chondrocytes were then seeded onto PGA and incubated in vitro for 1week. A 3x4 cm piece was cut from a decellularized aorta and four 0.5x3 cm pieces were excised from one side in a comb like fashion. A silicon stent was inserted into this structure and the spaces were filled with chondrocyte seeded PGA. The three dimensional cell-polymer construct was then implanted into a subcutaneous pocket of a nude rat for 4 weeks. Both native and TET were analyzed for sulfated glycosaminoglycan (S-GAG) and hydroxyproline content, and stained for H&E, Safranin-O and Collagen Type-II antibody. Results:The improved design of TET formed new cartilage rings in the structuralconfiguration of native trachea. Furthermore, the decellularized aorta connected well to the cartilage, which provided an excellent support to the rings, as well as good flexibility to the engineered trachea. Histological evaluation of TET showed the presence of mature cartilage. S-GAG and hydroxyproline content was similar to native cartilage levels. Conclusion:This study demonstrates the feasibility of engineering a trachea with defined cartilage rings and similar flexibility to the native trachea.

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Experimental tracheal replacement using tissue-engineered cartilage

Cited by 165 publications

References 15 publications

Tracheal Tissue Engineering

Tracheal Tissue Engineering

Electrospun gelatin/polycaprolactone nanofibrous membranes combined with a coculture of bone marrow stromal cells and chondrocytes for cartilage engineering

Tissue Engineered Trachea Using Decellularized Aorta

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