Experimental therapies for repair of the central nervous system: stem cells and tissue engineering

Forraz, Nico; Wright, K. E.; Jurga, Marcin; McGuckin, CP

doi:10.1002/term.552

Cited by 37 publications

(27 citation statements)

References 113 publications

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“…Biodegradable polymer scaffolds can act as a short‐term substitute for the extracellular matrix (ECM) and contribute to the regeneration of damaged tissue (Langer and Vacanti, ). Previous studies have revealed that tissue engineering would be useful to non‐invasively deliver the donor cells to the injured brain (Osanai et al ., ; Yasuda et al ., ; Forraz et al ., ). However, the technique still holds several problems.…”

Section: Introductionmentioning

confidence: 97%

Application of cell sheet technology to bone marrow stromal cell transplantation for rat brain infarct

Ito

Shichinohe

Houkin

et al. 2014

J Tissue Eng Regen Med

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Bone marrow stromal cells (BMSC) transplantation enhances functional recovery after cerebral infarct, but the optimal delivery route is undetermined. This study was aimed to assess whether a novel cell-sheet technology non-invasively serves therapeutic benefits to ischemic stroke. First, the monolayered cell sheet was engineered by culturing rat BMSCs on a temperature-responsive dish. The cell sheet was analysed histologically and then transplanted onto the ipsilateral neocortex of rats subjected to permanent middle cerebral artery occlusion at 7 days after the insult. Their behaviours and histology were compared with those in the animals treated with direct injection of BMSCs or vehicle over 4 weeks post-transplantation. The cell sheet was 27.9 ± 8.0 μm thick and was composed of 9.8 ± 2.4 × 10 cells. Cell sheet transplantation significantly improved motor function when compared with the vehicle-injected animals. Histological analysis revealed that the BMSCs were densely distributed to the neocortex adjacent to the cerebral infarct and expressed neuronal phenotype in the cell sheet-transplanted animals. These findings were almost equal to those for the animals treated with direct BMSC injection. The attachment of the BMSC sheet to the brain surface did not induce reactive astrocytes in the adjacent neocortex, although direct injection of BMSCs profoundly induced reactive astrocytes around the injection site. These findings suggest that the BMSCs in cell sheets preserve their biological capacity of migration and neural differentiation. Cell-sheet technology may enhance functional recovery after ischaemic stroke, using a less invasive method. Copyright © 2014 John Wiley & Sons, Ltd.

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Section: Introductionmentioning

confidence: 97%

Application of cell sheet technology to bone marrow stromal cell transplantation for rat brain infarct

Ito

Shichinohe

Houkin

et al. 2014

J Tissue Eng Regen Med

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“…Cell therapy is promising for repair of the damaged nervous system [1–3]. Bone marrow stromal cells (BMSCs) are candidate cells for such therapies because of their repair proficiency and relative accessibility [4–7].…”

Section: Introductionmentioning

confidence: 99%

The effect of a polyurethane-based reverse thermal gel on bone marrow stromal cell transplant survival and spinal cord repair

et al. 2014

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Cell therapy for nervous tissue repair is limited by low transplant survival. We investigated the effects of a polyurethane-based reverse thermal gel, poly(ethylene glycol)-poly(serinol hexamethylene urethane) (ESHU) on bone marrow stromal cell (BMSC) transplant survival and repair using a rat model of spinal cord contusion. Transplantation of BMSCs in ESHU at three days post-contusion resulted in a 3.5-fold increase in BMSC survival at one week post-injury and a 66% increase in spared nervous tissue volume at four weeks post-injury. These improvements were accompanied by enhanced hindlimb motor and sensorimotor recovery. In vitro, we found that ESHU protected BMSCs from hydrogen peroxide-mediated death, resulting in a four-fold increase in BMSC survival with two-fold fewer BMSCs expressing the apoptosis marker, caspase 3 and the DNA oxidation marker, 8-Oxo-deoxyguanosine. We argue that ESHU protected BMSCs transplanted is a spinal cord contusion from death thereby augmenting their effects on neuroprotection leading to improved behavioral restoration. The data show that the repair effects of intraneural BMSC transplants depend on the degree of their survival and may have a widespread impact on cell-based regenerative medicine.

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“…Stem cells transplant is a promising therapy for the repair of damaged nervous system [5][6][7]. Bone marrow derived mesenchymal stem cells (BMSCs) are one of the most commonly used cell to treat injured spinal cord.…”

Section: Introductionmentioning

confidence: 99%

Effect of mesenchymal stem cells transplantation combining with hyperbaric oxygen therapy on rehabilitation of rat spinal cord injury

Geng

Cao

Xiong

et al. 2015

Asian Pacific Journal of Tropical Medicine

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Experimental therapies for repair of the central nervous system: stem cells and tissue engineering

Cited by 37 publications

References 113 publications

Application of cell sheet technology to bone marrow stromal cell transplantation for rat brain infarct

Application of cell sheet technology to bone marrow stromal cell transplantation for rat brain infarct

The effect of a polyurethane-based reverse thermal gel on bone marrow stromal cell transplant survival and spinal cord repair

Effect of mesenchymal stem cells transplantation combining with hyperbaric oxygen therapy on rehabilitation of rat spinal cord injury

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