Experimental survey of non-clonogenic viability assays for adherent cells in vitro

Mickuviene, Ingrida; Kirvelienė, Vida; Juodka, Benediktas

doi:10.1016/j.tiv.2004.02.001

Cited by 44 publications

(30 citation statements)

References 17 publications

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“…Altered cell adhesion due to cellular damage can also be a useful parameter for evaluating short-term cytotoxicity. Indeed, measuring loss of monolayer adherence by fluorescent methods, proved as sensitive as assays measuring organelle-specific damage, for investigating cytotoxic effects of combined photodynamic therapy with chemotherapeutic drugs in different cell lines (28 …”

Section: Atp Assaymentioning

confidence: 99%

Cellular Chemosensitivity Assays: An Overview

Sumantran

2011

Methods in Molecular Biology

142

141

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Data on cell viability have long been obtained from in vitro cytotoxicity assays. Today, there is a focus on markers of cell death, and the MTT cell survival assay is widely used for measuring cytotoxic potential of a compound. However, a comprehensive evaluation of cytotoxicity requires additional assays which -measure short and long-term cytotoxicity. Assays which measure the cytostatic effects of compounds are not less important, particularly for newer anticancer agents. This overview discusses the advantages and disadvantages of different non-clonogenic assays for measuring short and medium-term cytotoxicity. It also discusses clonogenic assays, which accurately measure long-term cytostatic effects of drugs and toxic agents. For certain compounds and cell types, the advent of high throughput, multiparameter, cytotoxicity assays, and gene expression assays have made it possible to predict cytotoxic potency in vivo.

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Section: Atp Assaymentioning

confidence: 99%

Cellular Chemosensitivity Assays: An Overview

Sumantran

2011

Methods in Molecular Biology

142

141

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“…To apply the colony-forming assay with nonadherent cells is even more complicated, since cells have to be immobilized in gelatinous matrices if they grow at all. Furthermore, alternative methods like thymidine uptake, cell counting, bromo-desoxy-uridine (BrdU) labeling, or the MTT assay can quantify overall cell division, and viability, but do not record the division history of an individual cell [14,15,19,20].…”

Section: Discussionmentioning

confidence: 99%

The CFSE Distribution Assay is a Powerful Technique for the Analysis of Radiation–Induced Cell Death and Survival on a Single–Cell Level

et al. 2005

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“…The cytotoxic/cytostatic effects of proteasome inhibitors on melanoma cells or human primary fibroblasts were examined in vitro using the crystal violet assay, as previously described (12). Briefly, 5 Â 10 3 cells per well were seeded in 96-well microtiter plates in a total volume of 100 AL/well (Greiner) and proteasome inhibitor exposure was done the following day.…”

Section: Methodsmentioning

confidence: 99%