Molybdenum-added biomedical alloy has been prepared using a high-temperature vertical vacuum casting technique with five (0, 1, 2, 3, and 4 wt%) diverse weight percentages. The density, microhardness, and sliding wear behavior of the fabricated alloys were studied, showing that the addition of molybdenum content in the metal-metal alloy (i.e., Co-30Cr) increases the density from 7.2 to 8.7 g/cc for 0-4 wt% of Mo, respectively. Similarly, the hardness of prepared biomedical alloy also increases from 653 to 720 HV on addition of 0-4 wt% Mo particulate, respectively. The hardness is investigated by the microhardness tester. The aim of this current research work is to optimized the sliding wear behavior of molybdenum-added Co-30Cr alloy for implant material by Taguchi experimental design technique at five different normal loads (5-25 N), sliding velocities (0.26-1.3 m/s), sliding distance (500-2500 m), and reinforcement, i.e., Mo (0-4 wt%) respectively. To obtain the optimum wear response of prepared biomedical alloy added with Mo contents, the Taguchi L 25 orthogonal array was implemented. The wear test is performed on a pin-on-disc tribometer against a hardened alloy steel (EN-31) disc under different operating conditions at room temperature. Afterwards, field-emission scanning electron microscopy and atomic force microscopy were utilized to analyze the microstructure, contour of wear mechanism, and 3D surface topography of samples after test run.