Experimental study on repairing of nude mice skin defects with composite skin consisting of xenogeneic dermis and epidermal stem cells and hair follicle dermal papilla cells

Cryptomphalus aspersa Mollusc Egg Extract Promotes Regenerative Effects in Human Dermal Papilla Stem Cells

“…Thus, it is known that e-CAF also induces migration in the human keratinocyte cell line (HaCaT) and primary dermal fibroblasts and human senescent dermal fibroblasts, although no effects on cell proliferation were found; additionally, it produced an improvement in the organization of cytoskeletal proteins F-actin and vimentin and was able to promote the production of ECM components, such as FN and Col I [25]. This study shows that e-CAF could also have beneficial effects on human mesenchymal stem cells from dermal papilla (HHDPCs), improving the capabilities of this cell population, which are of great importance on skin regeneration, because of their multipotential ability to generate different skin lineages [29,30,31,32,33,34,35,36]. In fact, our results showed that treatment with 1 µg/mL of e-CAF for 28 days promoted cell proliferation and 0.1 µg/mL of e-CAF concentrations increased cell migration ability.…”

“…Additionally, HHDPCs act as an important repository for tissue repair after injury generation, where rapid and dynamic changes in cell microenvironment induce processes of cell differentiation and phenotypic plasticity [32,33]. Moreover, several studies with animal models point out the interest in dermal papilla cells as an important cell subpopulation of the hair follicle in regenerative processes of the skin [34,35] and, additionally, HHDPCs have been postulated as a suitable source for the construction of bioengineered skin substitutes in cases where autografting is not the appropriate option due to its immunosuppressive properties, multipotentiality, and plasticity to differentiate into several mesenchymal lineages [36]. Thus, the ability to maintain or improve the capabilities of this cell population seems to be of great interest for skin regeneration.…”

Functional Neovascularization in Tissue Engineering with Porcine Acellular Dermal Matrix and Human Umbilical Vein Endothelial Cells

“…18 Biological scaffolds derived from decellularized tissues have been successfully used in a variety of tissue engineering applications due to their composition and intrinsic growth factors. [19][20][21][22] These scaffolds are all composed of extracellular matrices but differ in their tissue source, species of origin, and methods by which they are processed. Previous studies have shown that decellularized matrices provide a compatible environment for human endothelial cell attachment, proliferation, and three-dimensional growth into tube-like vascular structures.…”

Hair follicle regeneration in vitro

“…More simplified models were required. The use of skin tissue equivalents for the study of folliculogenesis is complicated by the fact that human cells are incapable of stimulating suitable HF development in such models (Qi et al, 2008;Leirós et al, 2014). Alternatively, the researchers used artifi cial chimerical equivalents with mouse DP cells (Sri wiriyanont et al, 2013).…”