Experimental study of the mechanisms leading to the formation of glistenings in intraocular lenses by Raman spectroscopy

Rusciano, Giulia; Capaccio, Angela; Pesce, Giuseppe; Sasso, Antonio

doi:10.1364/boe.10.001870

Cited by 9 publications

(7 citation statements)

References 54 publications

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“…The broad band between 2600 cm −1 and 3200 cm −1 with maximal intensities at 2931 cm −1 and 3062 cm −1 arises from the convolution of symmetrical and asymmetrical stretching vibrations of CH and CH 3 bonds. The observed Raman spectra agrees with those available in the literature [46][47][48][49]. Raman spectra of samples processed on the surface and inside the IOL showed a strong diminution of Raman intensity.…”

Section: Microstructural and Compositional Characterizationsupporting

confidence: 89%

High-Repetition-Rate Femtosecond Laser Processing of Acrylic Intra-Ocular Lenses

Sola

Cases

2020

Polymers

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The study of laser processing of acrylic intra-ocular lenses (IOL) by using femtosecond laser pulses delivered at high-repetition rate is presented in this work. An ultra-compact air-cooled femtosecond diode laser (HighQ2-SHG, Spectra-Physics) delivering 250 fs laser pulses at the fixed wavelength of 520 nm with a repetition rate of 63 MHz was used to process the samples. Laser inscription of linear periodic patterns on the surface and inside the acrylic substrates was studied as a function of the processing parameters as well as the optical absorption characteristics of the sample. Scanning Electron Microscopy (SEM) Energy Dispersive X-ray Spectroscopy (EDX), and micro-Raman Spectroscopy were used to evaluate the compositional and microstructural changes induced by the laser radiation in the processed areas. Diffractive characterization was used to assess 1st-order efficiency and the refractive index change.

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Section: Microstructural and Compositional Characterizationsupporting

confidence: 89%

High-Repetition-Rate Femtosecond Laser Processing of Acrylic Intra-Ocular Lenses

Sola

Cases

2020

Polymers

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“…Light scattering may arise either due to the upsetting of lens microarchitecture or formation of protein aggregates. , Breakdown of lens registry includes vacuole formation and degeneration of lens fiber cells while light scattering due to accumulation of protein aggregates occurs when they grow beyond 1000 Å in size . While the former is of great clinical interest to ophthalmologists, lens protein aggregation is a subject of intense biophysical research.…”

Section: Introductionmentioning

confidence: 99%

A Perspective on Biophysical Studies of Crystallin Aggregation and Implications for Cataract Formation

Bari

Sharma

2020

J. Phys. Chem. B

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Lens crystallins are subject to various types of damage during their lifetime which triggers protein misfolding and aggregation, ultimately causing cataracts. There are several models for crystallin aggregation, but a comprehensive picture of the mechanism of cataract is still underway. The complex biomolecular interactions underlying crystallin aggregation have motivated major efforts to resolve the structural details and mechanism of aggregation using multiple biophysical techniques at different resolutions. Together, experimental and computational approaches identify and characterize both amyloidogenic and amorphous aggregates leading to an improved understanding of crystallin aggregation. A rigorous characterization of the aggregation-prone intermediates is crucial in cataract-mediated drug discovery. This Perspective summarizes recent biophysical studies on lens crystallin aggregation. We evaluate the outstanding challenges, future outlook, and rewards in this fertile field of research. With lessons learned from protein folding and multiple pathways of aggregation, we highlight the differences in the overall mechanisms of age-related and congenital cataracts. We expect that a correlation between the existing and developing biophysical techniques would provide a platform to study amyloid architecture in the eye lens and reduce the existing gaps in our understanding of crystallin biophysics.

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“…For instance, PMMA is used to confer more rigidity to the system, whereas PEMA to furnish a certain elasticity. Finally, the presence of the OH group in poly-HEMA enhances the system hygroscopic properties [ 44 ].…”

Section: Iols Materials Marketmentioning

confidence: 99%

Current State of the Art and Next Generation of Materials for a Customized IntraOcular Lens according to a Patient-Specific Eye Power

et al. 2023

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Intraocular lenses (IOLs) are commonly implanted after surgical removal of a cataractous lens. A variety of IOL materials are currently available, including collamer, hydrophobic acrylic, hydrophilic acrylic, PHEMA copolymer, polymethylmethacrylate (PMMA), and silicone. High-quality polymers with distinct physical and optical properties for IOL manufacturing and in line with the highest quality standards on the market have evolved to encompass medical needs. Each of them and their packaging show unique advantages and disadvantages. Here, we highlight the evolution of polymeric materials and mainly the current state of the art of the unique properties of some polymeric systems used for IOL design, identifying current limitations for future improvements. We investigate the characteristics of the next generation of IOL materials, which must satisfy biocompatibility requirements and have tuneable refractive index to create patient-specific eye power, preventing formation of posterior capsular opacification.

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Experimental study of the mechanisms leading to the formation of glistenings in intraocular lenses by Raman spectroscopy

Cited by 9 publications

References 54 publications

High-Repetition-Rate Femtosecond Laser Processing of Acrylic Intra-Ocular Lenses

High-Repetition-Rate Femtosecond Laser Processing of Acrylic Intra-Ocular Lenses

A Perspective on Biophysical Studies of Crystallin Aggregation and Implications for Cataract Formation

Current State of the Art and Next Generation of Materials for a Customized IntraOcular Lens according to a Patient-Specific Eye Power

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