Experimental study of the interstitial effect of a semiconductor laser with a wavelength of 445 nm on a biological model

Abstract

Introduction. This study presents the results of interstitial exposure of a semiconductor laser with a wavelength of 445 nm to biological tissue samples at different pulsed wave power in a constant mode, with a contact method.Aim. To study the interstitial effect of a semiconductor laser with a wavelength of 445 nm on experimental tissue samples in a constant mode at different power.Materials and methods. As an experimental sample, we used biological tissue with a developed vascular structure in the form of pig liver. The source of laser radiation was a semiconductor laser with a wavelength of 445 nm, with a power range from 0.5 to 4 watts. When working with biological tissue samples, we evaluated their external and internal changes after laser exposure. The exposure time during interstitial exposure was 1 mm/sec with a 20 mm immersion depth of the laser fiber into the fabric. The results of the macro and microscopic picture were evaluated using histological examination and morphometry of the zones of destruction and coagulation necrosis, on a transverse section of the tissue.Results. The results of an experimental study indicate that interstitial laser exposure has a pronounced coagulation effect combined with a cutting effect. The optimal combination of coagulation and cutting effect of exposure, accompanied by visual contractility of the tissue, without excessive carbonation at a power of 3.0 watts.Conclusion. The use of interstitial exposure of a semiconductor laser with a wavelength of 445 nm on experimental tissue samples in a constant mode at different power showed the predominance of the coagulation effect in combination with the cutting effect with a pronounced reduction in tissue volume. Experiments have shown that the power of 3 W is the optimal power of laser exposure in the interstitial method, in which there is a pronounced reduction in the volume of the studied drug without excessive carbonation.