Experimental study of the combined effects of pH and salinity on the bioaccumulation of inorganic mercury in the crayfish<i>Astacus leptodactylus</i>

Laporte, Jean-Michel; Ribeyre, Francis; Truchot, J.P.; Boudou, A.

doi:10.1080/09542299.1996.11083261

Cited by 7 publications

(4 citation statements)

References 30 publications

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“…For MeHg, a plateau was reached after 40 d: the differences between the three average concentrations measured at 40, 60, and 80 d are not statistically significant (one‐way ANOVA and least‐square deviation test). For Hg(II), the plateau was less distinctive: only the averages measured after 60 and 80 d of exposure were not statistically different; however, they were significantly higher than those at 40 d. These results are in agreement with earlier studies carried out in comparable conditions, with Hg concentrations that were nevertheless considerably higher in the water column [21]. These two accumulation kinetics enable us to determine bioconcentration factors ([Hg] soft body (fresh weight) /[Hg] d or [Hg] Tot (water) ) at the plateau phase (60 or 80 d).…”

Section: Resultssupporting

confidence: 91%

“…These results are in agreement with the data available in the literature for other species of crustaceans. Thus, the gills represent the route via which the Hg(II) entered the organism and the gills have a very high accumulation capacity, given that they have a vast surface area for exchanges with the external environment and the almost permanent renewal of water in the gill cavity [21,24,25]. Autometallography studies on the gills of the common shore crab ( Carcinus maenas ) after direct exposure of the organisms or contamination of isolated and perfused gills, have shown that Hg(II) was accumulated in the superficial layers of the cuticule and within vacuoles in the nephrocytes, with this cell type playing an important phagocytic role [26].…”

Section: Resultsmentioning

confidence: 99%

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Simultaneous experimental study of direct and direct plus trophic contamination of the crayfish astacus astacus by inorganic mercury and methylmercury

Simon

Boudou

2001

Enviro Toxic and Chemistry

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An experimental study was carried out to investigate direct and direct plus trophic contamination routes of the crayfish Astacus astacus by inorganic mercury (Hg(II)) or methylmercury (MeHg). Direct exposure was based on low contamination conditions, 300 and 30 ng/L in the dissolved phase, respectively, during 30 d at 20 degrees C. Trophic exposure was based on daily consumption of the Asiatic clam Corbicula fluminea, previously contaminated during 40 d with similar exposure conditions. The Hg concentrations in the bivalves were very similar: 1,451 +/- 287 ng/g for Hg(II) and 1,346 +/- 143 ng/g for MeHg. In the crustaceans, Hg bioaccumulation was analyzed at the whole-organism level and in eight organs (gills, stomach, intestine, hepatopancreas, tail muscle, green gland, carapace, and hemolymph), after 15 and 30 d of exposure. Analysis of the results showed marked differences between Hg(II) and MeHg accumulation in favor of MeHg: for the direct route, the ratio between metal concentrations was close to 8; for the trophic route, no significant increase in Hg accumulation was observed for Hg(II) even when the ratio between Hg concentration in the direct plus trophic contamination route and Hg concentration in the direct contamination route was 1.6 for MeHg, with an estimated trophic transfer rate close to 20%. Mercury organotropism was also specifically connected to the exposure conditions, especially at the biological barrier level according to the route of exposure: gills and carapace for the direct route and digestive tract including hepatopancreas for the trophic route.

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Simultaneous experimental study of direct and direct plus trophic contamination of the crayfish astacus astacus by inorganic mercury and methylmercury

Simon

Boudou

2001

Enviro Toxic and Chemistry

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“…It is commonly thought that the main route of entry of trace metals from the ambient medium is the gill epithelium, and recent studies have shown that this is indeed the case for mercury in crabs (Barradas & Pequeux 1996. Furthermore, a number of other studies indicate that the gill tissue of several crustacean species is able to accumulate very high amounts of mercury following direct contamination from the ambient water (McLusky & Bryant 1986, Canli & Furness 1995, Wright 1995, Laporte et al 1996. In the shore crab Carcinus maenas for example, 15 d exposure to 1 µg l -1 of either inorganic or methylmercury resulted in gill bioconcentration factors of up to 1500 -3000, corresponding to gill metal concentrations that were 10 to 50 times higher than in the carapace and internal organs.…”

Section: Introductionmentioning

confidence: 99%

Bioaccumulation of inorganic and methylated mercury by the gills of the shore crab Carcinus maenas: transepithelial fluxes and histochemical localization

Laporte¹,

Truchot

Mesmer-Dudons³

et al. 2002

Mar. Ecol. Prog. Ser.

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In order to better understand the role of the gills in the accumulation of contaminants, branchial uptake and bioaccumulation of inorganic (HgCl 2 ) and monomethylmercury (CH 3 HgCl) were quantified in the shore crab Carcinus maenas, using living animals and an in vitro perfused gill preparation exposed to 50 µg l -1 of either chemical forms in the external medium. In addition, localization of accumulated mercury was studied using the histochemical autometallographic technique by both light and electron microscopy. Gill tissue strongly accumulated either inorganic or methylated mercury at similar levels in vitro and in vivo. For both chemical forms of the metal, only about 1% of the total mercury input was recovered in the effluent fluid from in vitro perfused gills and could thus be considered available to distribute inside the animal via the circulatory system. Inorganic Hg was histochemically found to be accumulated at 2 markedly different locations in the gills: at the cuticular surface in direct contact with the contaminated medium and at high local levels in the central vacuole of gill nephrocytes. Although also present at these 2 locations, methylmercury was distributed more diffusely and more evenly in all cells. These results suggest that the high affinity of gill tissue for both forms of mercury may confer on this organ the role of an external barrier strongly limiting the invasion of the metal toward other compartments of the body.

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“…Gills were also identified as the second organ after the hepatopancreas, able to accumulate metals after exposure to high concentration (Rainbow, 1995;Laporte et al, 1996;Soegianto et al, 1999). They are involved in removing foreign particles (Martin et al, 2000), and in penaeid shrimp they are the major site of removal of foreign materials, because shrimps lack fixed phagocytes in haemal spaces of the digestive gland (Martin et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

Gill morphology and ultrastructure of the prawn, Palaemonetes argentinus Nobili, 1901 (Decapoda, Caridea)

Petriella

Sousa

2005

Crustac

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A morphological and ultrastructural study was performed on the gills of Palaemonetes argentinus from the Sotelo stream, a tributary of the Mar Chiquita coastal lagoon (Argentina, 37 • 45 S 57 • 26 W). This species possesses a phyllobranchiate gill, in which the central axis is triangular and has one row of flattened lamellae on each side. There are one afferent channel, two efferent channels, and several tegumental glands along the axis. Nephrocytes are only present in the stem, associated with the efferent haemolymph. Mature nephrocytes have a voluminous vacuole, surrounded by satellite vacuoles, a lateral nucleus, and pedicels on the surface. In the lamellae, the principal cells have apical extensions with deep infoldings and a neck-like zone with bundles of microtubules. The nucleus is in a basal expansion of the cell and the basolateral membranes of neighbouring cells are interdigitated, with large numbers of mitochondria in the interdigitations. The cells show characteristics of both respiratory and ion-regulatory epithelium; they also have a structural role in directing the haemolymph flow and in supporting the lamellae. The thicker cuticle near the marginal channel increases mechanical support. The ultrastructure of the lamellar epithelium appears to be related to the capability of P. argentinus of living in freshwater and adapting to media with variable salinity. RESÚMENSe realizó un estudio morfológico y ultraestructural de las branquias del camarón Palaemonetes argentinus del arroyo Sotelo, tributario de la laguna costera de Mar Chiquita (Argentina, 37 • 45 S 57 • 26 W). Esta especie posee filobranquias, cuyo eje central tiene forma triangular y posee una hilera de laminillas aplanadas a cada lado. Hay un canal aferente, dos canales eferentes y glándulas tegumentarias a lo largo del eje. Se observaron nefrocitos asociados con la hemolinfa eferente en el eje central. Los nefrocitos maduros tienen una vacuola voluminosa rodeada por vacuolas satélite, un núcleo lateral y pedicelos en la superficie. En las laminillas las células principales tienen extensiones apicales con profundos pliegues y una zona más estrecha con numerosos microtúbulos. El núcleo se ubica en una expansion basal y las membranas basolaterales de las células vecinas se interdigitan encerrando numerosas mitocondrias. Estas células muestran características de un epitelio respiratorio y osmorregulador. También tienen una función estructural: dirigen el flujo de la hemolinfa y 3 )

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Experimental study of the combined effects of pH and salinity on the bioaccumulation of inorganic mercury in the crayfishAstacus leptodactylus

Cited by 7 publications

References 30 publications

Simultaneous experimental study of direct and direct plus trophic contamination of the crayfish astacus astacus by inorganic mercury and methylmercury

Simultaneous experimental study of direct and direct plus trophic contamination of the crayfish astacus astacus by inorganic mercury and methylmercury

Bioaccumulation of inorganic and methylated mercury by the gills of the shore crab Carcinus maenas: transepithelial fluxes and histochemical localization

Gill morphology and ultrastructure of the prawn, Palaemonetes argentinus Nobili, 1901 (Decapoda, Caridea)

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