Experimental Study of Neuroprotective Properties of Inhaled Argon-Oxygen Mixture in a Photoinduced Ischemic Stroke Model

Boeva, E. A.; Silachev, D. N.; Yakupova, E. I.; Milovanova, M. A.; Varnakova, L. A.; Kalabushev, S. N.; Denisov, S. O.; Antonova, V. V.; Ryzhkov, I. A.; Lapin, K. N.; Grebenchikova, A. A.

doi:10.15360/1813-9779-2023-3-46-53

Cited by 2 publications

(1 citation statement)

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“…In addition, the mechanisms underlying xenon-induced neuroprotection have been extensively studied [ 23 , 24 ]. For argon, there are far fewer studies [ 22 , 25 , 26 ], and they do not always clearly indicate the presence of protective effects on the brain [ 8 , 9 , 21 , 27 ]. Few studies have demonstrated the neuroprotective potential of neon and krypton [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

Neuroprotective Effects of Krypton Inhalation on Photothrombotic Ischemic Stroke

Antonova,

Silachev,

Plotnikov

et al. 2024

Biomedicines

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This is the first in vivo study to investigate the neuroprotective effects of krypton on focal cerebral ischemia. The aim of the study was to analyze the effect of 2 h of inhalation of a krypton–oxygen mixture (Kr 70%/O2 30%) on the recovery of neurological functions and the degree of brain damage in rats after photoinduced ischemic stroke (PIS) and to investigate the possible mechanisms responsible for this neuroprotection. Experiments were performed on male Wistar rats weighing 250–300 g (n = 32). Animals were randomized into four groups. Two groups (n = 20) underwent photoinduced ischemic stroke, followed by 2 h of inhalation of krypton–oxygen mixture consisting of Kr 70%/O2 30% or a nitrogen–oxygen breathing mixture consisting of N2 70%/O2 30%, followed by neurological examinations on days 3 and 7. The other two groups (n = 12) received only gas mixtures of the same concentration and exposure duration as in those in the PIS groups, then Western blot analysis of the potential molecular mechanisms was performed. The results of the study show that treatment with the krypton–oxygen mixture consisting of Kr 70%/O2 30% improves the neurological status on day 7 of observation, reduces the lesion volume according to the MRI examination and the number of Iba-1- and caspase-3-positive cells in the damaged area, promotes the activation of neoangiogenesis (an increase in the von Willebrand factor), and reduces the penumbra area and the number of NeuN-positive cells in it on day 14 of observation. Inhalation of the krypton–oxygen mixture also significantly increases the levels of phosphorylated AKT kinase (protein kinase B) and glycogen synthase kinase 3b (pGSK3b) and promotes the expression of transcription factor Nrf2, which was accompanied by the lowered expression of transcription factor NFkB (p50). Thus, we showed pronounced neuroprotection induced by krypton inhalation after stroke and identified the signaling pathways that may be responsible for restoring neurological functions and reducing damage.

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Section: Discussionmentioning

confidence: 99%

Neuroprotective Effects of Krypton Inhalation on Photothrombotic Ischemic Stroke

Antonova,

Silachev,

Plotnikov

et al. 2024

Biomedicines

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Nueroprotection by Anesthetics in Brain Injury Models

Bocharnikov,

Boeva,

Milovanova

et al. 2024

Obŝaâ reanimatologiâ

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The aim of the study was to compare the eﬀect of sevoﬂurane and chloral hydrate on the neurological status and volume of brain damage after trauma and ischemia in experimental models of traumatic brain injury (TBI) and focal ischemic stroke (IS) induced by photothrombosis (PT).Materials and methods. The experiments were performed on mongrel Wistar rats weighing 250–300 g (N=43). There were 4 groups: the Ischemia + Sevoﬂurane group (ISSEV) (N=10), the Ischemia + Chloral hydrate group (ISCH) (N=10), TBI + Sevoﬂurane group (TBISEV) (N=13), and TBI+Chloral hydrate group (TBICH) (N=10). Ischemic brain damage was modelled using Rose Bengal (RB) dye-induced PT, and TBI was modelled using mechanical force-induced concussion.Results. MRI findings indicate lower volumes of brain damage (mm³) in rats from TBISEV group compared with the TBICH group (19±5 vs. 60±5, P<0.0001), and in the ISSEV group compared with the ISCH group (9.8±1.5 vs. 21.5±2, P=0.0016). Moreover, there was a significant difference between ISSEV and ISCH groups based on the protocol assessment of neurological status on day 14 with higher scores in ISSEV (11.4±1.8 vs. 4.9±2.6, P<0.0001).Conclusion. Taking into account the data obtained, we recommend a careful choice of anesthesia when modeling ischemic stroke and traumatic brain injury in animals. In particular, the neuroprotective eﬀect of sevoﬂurane should be taken into account in the PT and TBI models.

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Experimental Study of Neuroprotective Properties of Inhaled Argon-Oxygen Mixture in a Photoinduced Ischemic Stroke Model

Cited by 2 publications

References 54 publications

Neuroprotective Effects of Krypton Inhalation on Photothrombotic Ischemic Stroke

Neuroprotective Effects of Krypton Inhalation on Photothrombotic Ischemic Stroke

Nueroprotection by Anesthetics in Brain Injury Models

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