Experimental Study of Bone Lengthening in Dogs by Means of Backscattered Scanning Electron Microscopy

Lafuente, Pilar; Franch, Jordi; Durall, I.; Manzanares, Cristina

doi:10.1111/j.1532-950x.2009.00505.x

Cited by 6 publications

(8 citation statements)

References 37 publications

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“…It has a faster calcification and contains types I and II collagen fibers [65,66]. Woven, lamellar, and osteonal bone are the calcified tissues that replace chondroid bone in our histological and BS-SEM findings, in a similar manner to the one seen in other endomembranous ossification-based processes, such as mandibular symphysis closure [62], skull suture growth and closure [67,68,69], tooth eruption [70,71], fracture repair [59,60,61,62,63,64,65,66,67], and, more recently, the osseointegration of non-metallic biomaterials [14,35].…”

Section: Discussionsupporting

confidence: 74%

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Biofunctionalization with a TGFβ-1 Inhibitor Peptide in the Osseointegration of Synthetic Bone Grafts: An In Vivo Study in Beagle Dogs

et al. 2019

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Objectives: The aim of this research was to determine the osseointegration of two presentations of biphasic calcium phosphate (BCP) biomaterial—one untreated and another submitted to biofunctionalization with a TGF-β1 inhibitor peptide, P144, on dental alveolus. Materials and Methods: A synthetic bone graft was used, namely, (i) Maxresorb® (Botiss Klockner) (n = 12), and (ii) Maxresorb® (Botiss Klockner) biofunctionalized with P144 peptide (n = 12). Both bone grafts were implanted in the two hemimandibles of six beagle dogs in the same surgical time, immediately after tooth extraction. Two dogs were sacrificed 2, 4, and 8 weeks post implant insertion, respectively. The samples were submitted to histomorphometrical and histological analyses. For each sample, we quantified the new bone growth and the new bone formed around the biomaterial’s granules. After optical microscopic histological evaluation, selected samples were studied using backscattered scanning electron microscopy (BS-SEM). Results: The biofunctionalization of the biomaterial’s granules maintains a stable membranous bone formation throughout the experiment timeline, benefitting from the constant presence of vascular structures in the alveolar space, in a more active manner that in the control samples. Better results in the experimental groups were proven both by quantitative and qualitative analysis. Conclusions: Synthetic bone graft biofunctionalization results in slightly better quantitative parameters of the implant’s osseointegration. The qualitative histological and ultramicroscopic analysis shows that biofunctionalization may shorten the healing period of dental biomaterials.

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Section: Discussionsupporting

confidence: 74%

“…The tissues of the first trabeculae that surround the granules as well as the ones formed as a response to the periosteal injury are mainly constituted by chondroid bone [64]. It has a faster calcification and contains types I and II collagen fibers [65,66].…”

Section: Discussionmentioning

confidence: 99%

Biofunctionalization with a TGFβ-1 Inhibitor Peptide in the Osseointegration of Synthetic Bone Grafts: An In Vivo Study in Beagle Dogs

et al. 2019

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“…The presence of chondroid tissue, also described by Bailleul et al [ 25 ] as “chondroid bone”, in the initial trabeculae surrounding the biomaterial ( Fig 5 ), as identified by back scattered SEM [ 26 ], cannot necessarily be interpreted as endochondral ossification. In fact, it has been demonstrated that chondroid tissue is an essential part of the human skull membranous ossification [ 27 , 28 ] and other repair processes [ 29 , 30 ], even if it can be present also in endochondral ossification. Moreover Bailleul et al [ 25 ] described very thoroughly the evolution, morphological characteristics, and participation of chondroid tissue in endomembranous ossification in different species, as they have been described in the literature.…”

Section: Discussionmentioning

confidence: 99%

In Vivo Osteogenic Potential of Biomimetic Hydroxyapatite/Collagen Microspheres: Comparison with Injectable Cement Pastes

et al. 2015

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The osteogenic capacity of biomimetic calcium deficient hydroxyapatite microspheres with and without collagen obtained by emulsification of a calcium phosphate cement paste has been evaluated in an in vivo model, and compared with an injectable calcium phosphate cement with the same composition. The materials were implanted into a 5 mm defect in the femur condyle of rabbits, and bone formation was assessed after 1 and 3 months. The histological analysis revealed that the cements presented cellular activity only in the margins of the material, whereas each one of the individual microspheres was covered with osteogenic cells. Consequently, bone ingrowth was enhanced by the microspheres, with a tenfold increase compared to the cement, which was associated to the higher accessibility for the cells provided by the macroporous network between the microspheres, and the larger surface area available for osteoconduction. No significant differences were found in terms of bone formation associated with the presence of collagen in the materials, although a more extensive erosion of the collagen-containing microspheres was observed.

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“…Whereas in distraction gaps the tissue between the ends of the mineralised bone is first subject to bleeding, coagulation, granulation tissue and fibrosis (Kokich, 1976;Shapiro, 2008), bone directly develops at the suture edges in craniosynostosis. In addition, in contrast to the distraction gap (Yasui et al, 1997;Jazrawi et al, 1998;Sato et al, 1998;Ploder et al, 2002;Lafuente et al, 2009;Forriol et al, 2010), our analyses of sutural sites showed no verification of enchondral and transchondroid types of osteogenesis.…”

Section: J Regelsberger Et Almentioning

confidence: 41%

Changes to the cell, tissue and architecture levels in cranial suture synostosis reveal a problem of timing in bone development

Regelsberger¹,

Milovanović²,

Schmidt³

et al. 2012

eCM

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Premature fusion of cranial sutures is a common problem with an incidence of 3-5 per 10,000 live births. Despite progress in understanding molecular/genetic factors affecting suture function, the complex process of premature fusion is still poorly understood. In the present study, corresponding excised segments of nine patent and nine prematurely fused sagittal sutures from infants (age range 3-7 months) with a special emphasis on their hierarchical structural configuration were compared. Cell, tissue and architecture characteristics were analysed by transmitted and polarised light microscopy, 2D-histomorphometry, backscattered electron microscopy and energy-dispersivex-ray analyses. Apart from wider sutural gaps, patent sutures showed histologically increased new bone formation compared to reduced new bone formation and osseous edges with a more mature structure in the fused portions of the sutures. This pattern was accompanied by a lower osteocyte lacunar density and a higher number of evenly mineralised osteons, reflecting pronounced lamellar bone characteristics along the prematurely fused sutures. In contrast, increases in osteocyte lacunar number and size accompanied by mineralisation heterogeneity and randomly oriented collagen fibres predominantly signified woven bone characteristics in patent, still growing suture segments. The already established woven-to-lamellar bone transition provides evidence of advanced bone development in synostotic sutures. Since structural and compositional features of prematurely fused sutures did not show signs of pathological/defective ossification processes, this supports the theory of a normal ossification process in suture synostosis -just locally commencing too early. These histomorphological findings may provide the basis for a better understanding of the pathomechanism of craniosynostosis, and for future strategies to predict suture fusion and to determine surgical intervention.

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Experimental Study of Bone Lengthening in Dogs by Means of Backscattered Scanning Electron Microscopy

Abstract: BS-SEM is an effective technique for studying progression of bone healing during DO. The presence of chondroid tissue during DO explains why callus mineralization occurs more rapidly during distraction than during static stabilization.

Cited by 6 publications

References 37 publications

Biofunctionalization with a TGFβ-1 Inhibitor Peptide in the Osseointegration of Synthetic Bone Grafts: An In Vivo Study in Beagle Dogs

Biofunctionalization with a TGFβ-1 Inhibitor Peptide in the Osseointegration of Synthetic Bone Grafts: An In Vivo Study in Beagle Dogs

In Vivo Osteogenic Potential of Biomimetic Hydroxyapatite/Collagen Microspheres: Comparison with Injectable Cement Pastes

Changes to the cell, tissue and architecture levels in cranial suture synostosis reveal a problem of timing in bone development

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