Experimental study and preliminary economic evaluation of enzymatic biodiesel production by an integrated process using co-products from palm (Elaeais guineensis Jaquim) industry

Collaço, Ana Cristina A.; Aguieiras, Érika C.G.; Santos, Juliana Gomes; Oliveira, Renata Andrade de; Castro, Rui de Paula Vieira de; Freire, Denise Maria Guimarães

doi:10.1016/j.indcrop.2020.112904

Cited by 19 publications

(5 citation statements)

References 22 publications

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“…This combination of three modules CsgA, SpyTag/SpyCatcher, and Mr cp19k collaboratively offer a wealth of nanoscale specific area and robust stable environment, which could serve as potential key characteristics for biocatalysis. The utilization of waste cooking oils containing high levels of free fatty acids for FAMEs production poses significant challenges, including low yield, increased dosages of catalyst and short-chain alcohols or equipment corrosion issues during acidic (H 2 SO 4 ) or basic (NaOH and KOH) based chemical catalysis. − Furthermore, the intricate separation process and discharge of acidic, basic, and alcoholic waste solutions hinder the economic viability and environmental sustainability of this process. , However, the three modules (CsgA, SpyTag/SpyCatcher, and Mr cp19k) based genetically programmed lipase offers a robust, cost-effective, environmentally friendly and sustainable route for converting waste cooking oils into FAMEs.…”

Section: Resultsmentioning

confidence: 99%

“…29−31 Furthermore, the intricate separation process and discharge of acidic, basic, and alcoholic waste solutions hinder the economic viability and environmental sustainability of this process. 32,33 However, the three modules (CsgA, SpyTag/SpyCatcher, and Mrcp19k) based genetically programmed lipase offers a robust, cost-effective, environmentally friendly and sustainable route for converting waste cooking oils into FAMEs.…”

Section: Csga-spytag/spycatcher-mrcp19k Based Whole Cell Tll (Type Iii)mentioning

confidence: 99%

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Genetically Decorating Microbial Surface Curli Fiber with Biocatalytic Properties

Wang,

Shi,

Liu

et al. 2024

ACS Sustainable Chem. Eng.

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Genetically customized microbial surface represents an emerging strategy for creating effective and robust whole cell biocatalysts. In this study, we have genetically engineered new whole cell catalysts that utilize three functional modules: CsgA, SpyTag/SpyCatcher, and Mrcp19k. These modules can programmatically immobilize lipases onto the surface curli fibers of Escherichia coli. The genetically programmed E. coli surface curli fibers exhibited enhanced lipase enzyme dosage, specific activity, and resistance to harsh environments. The application of these genetically tailored whole cell catalysts in the synthesis of fatty acid methyl esters resulted in an 87% yield, with more than 80% relative activity retained after 5 consecutive batches. This genetically programmable immobilization strategy, particularly the integration of Mrcp19k, introduces a concept and technological platform that enables the creation of innovative and highly efficient whole cell biocatalysts and biotransformations.

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Section: Resultsmentioning

confidence: 99%

Section: Csga-spytag/spycatcher-mrcp19k Based Whole Cell Tll (Type Iii)mentioning

confidence: 99%

Genetically Decorating Microbial Surface Curli Fiber with Biocatalytic Properties

Wang,

Shi,

Liu

et al. 2024

ACS Sustainable Chem. Eng.

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“…Attention must be given to the costs of the biocatalysts, which must be reduced to make enzymatic biodiesel competitive. Thus, technologies for the use of solid (Collaço et al, 2020) and liquid oil crops by-products (Robert et al, 2017), as well as the immobilization of lipases on non-commercial supports (Pinto et al, 2020), must be studied and improved, aiming the obtention of the ideal biocatalyst in terms of cost, activity, and stability.…”

Section: Product Characterizationmentioning

confidence: 99%

Fatty acid ethyl esters production from distillers corn oil by enzymatic catalysis

Aguieiras

Abreu

Oliveira

et al. 2022

J Americ Oil Chem Soc

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Distillers corn oil (DCO), a by-product from the corn ethanol production, is an alternative source of glycerides to produce biodiesel. In the present work, this feedstock was enzymatically converted into fatty acid ethyl esters (FAEE). DCO presented relative density of 0.912 g/cm 3 , peroxide index of 3.05 AE 0.3 (meq/1000 g of sample) and acidity of 13.1 AE 1.96 (wt.%), with prevalence of the oleic (18:1) and linoleic (18:2) acids (sum equal to 76.9%). Three strategies were evaluated to improve the FAEE content: ethanol stepwise addition (ethanol:oil molar ratio of 4:1 (1/2 of ethanol at 0 and 1.5 h) and 3:1 (1/3 of ethanol at 0 h and 2/3 at 1.5 h or 1/3 of ethanol at 0, 1 and 2 h)); consecutive reactions (with Novozym 40086 [Rhizomucor miehei lipase] as biocatalyst); and mixture of enzymes (Novozym 40086 (6 wt.%) + Novozym 435 (Candida antarctica B lipase) (2 wt.%). The concept of combi-lipases was evaluated for the first time using this raw material, resulting in a high ester content (>96%). The higher ester yields attained with DCO, compared to refined corn oil (33%) can be related to the better solubilization of ethanol and/or glycerol by-product in the medium with DCO. The combi-lipase system kept high than 80% of this initial activity after three repeated batches of reaction. The use of DCO enables the integration of corn ethanol and biodiesel production within the same general facility, which has logistic, environmental and, economic relevance.

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“…Particularly, the use of enzymes as catalysts to carry out such biotransformations constitutes an environmentally attractive alternative and, often, economically viable route. 6,7 The use of enzymes enables the generation of purer products, the increase of energy efficiency (since milder operating conditions of temperature and pressure may be employed) and, often, the reduction of production costs (since, in general, there is a reduction in the number of reaction steps, an increase in the reaction yields and it is possible to use less expensive feedstocks). [6][7][8] Lipases have been widely used in distinct industrial sectors, including biodiesel industries, once these catalysts may exhibit high activity, specificity, and selectivity under mild reaction conditions.…”

Section: Introductionmentioning

confidence: 99%

“…6,7 The use of enzymes enables the generation of purer products, the increase of energy efficiency (since milder operating conditions of temperature and pressure may be employed) and, often, the reduction of production costs (since, in general, there is a reduction in the number of reaction steps, an increase in the reaction yields and it is possible to use less expensive feedstocks). [6][7][8] Lipases have been widely used in distinct industrial sectors, including biodiesel industries, once these catalysts may exhibit high activity, specificity, and selectivity under mild reaction conditions. [8][9][10][11] This way, lipase B from Candida antarctica is a versatile enzyme of great commercial interest, being employed in several biotechnological processes.…”

Section: Introductionmentioning

confidence: 99%

Polymerization strategies to produce new polymer biocatalysts for the biodiesel industry

Pinto

Sousa

Dutra

et al. 2021

J of Applied Polymer Sci

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One of the major challenges regarding the enzymatic production of biodiesel is the development of more robust, active, and stable immobilized biocatalysts. Thus, the present work aims to develop new enzymatic biocatalysts for use in esterification and transesterification reactions. New polymer supports based on poly(styrene-co-divinylbenzene) and poly(methyl methacrylate-co-divinylbenzene) platforms were synthesized, incorporating distinct functional compounds into the polymer chains (1-octene, cardanol, and vinyl benzoate). Two distinct polymerization strategies were adopted for support syntheses: (i) Combined suspension and emulsion polymerization process (core-shell particles); and (ii) Two-step polymerization reaction comprising a suspension polymerization in presence of porogenic agents, followed by vacuum application (porous and nonporous particles). The obtained particles were employed for immobilization of lipase B from Candida antarctica. The addition of functional compounds resulted in particles with distinct textural properties. Moreover, particles with 91 m 2 .g À1 (P(MMA-co-DVB)/ P(MMA-co-DVB)) were produced through combined suspension and emulsion polymerization, whilst particles with 154 m 2 .g À1 (P[MMA-co-DVB-co-VB]) were produced through suspension polymerization performed in presence of n-heptane. Moreover, highly active biocatalysts were produced, leading to esterification conversions above 80%. Thus, based on the performance in esterification and transesterification reactions, the new functional matrices resulted in highly active biocatalysts with good potential for use in biodiesel industry.

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Experimental study and preliminary economic evaluation of enzymatic biodiesel production by an integrated process using co-products from palm (Elaeais guineensis Jaquim) industry

Cited by 19 publications

References 22 publications

Genetically Decorating Microbial Surface Curli Fiber with Biocatalytic Properties

Genetically Decorating Microbial Surface Curli Fiber with Biocatalytic Properties

Fatty acid ethyl esters production from distillers corn oil by enzymatic catalysis

Polymerization strategies to produce new polymer biocatalysts for the biodiesel industry

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