Experimental Studies of the Protective Effect of Ginkgo Biloba Extract(GBE) on Cisplatin-induced Toxicity in Rats.

Fukaya, Hirotomo; Kanno, Hidetaka

doi:10.3950/jibiinkoka.102.907

Cited by 32 publications

(30 citation statements)

References 24 publications

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“…It was shown that levels of glutathione (GSH) and antioxidant enzymes as superoxide dismutase (SOD), catalase, GSH peroxidase and GSH reductase are reduced in these tissues leading to lipid peroxidation, thus, to cellular toxicity. 3,12,15,17,18 Anti-oxidant effects of Hyperbaric Oxygen Therapy have been observed through decrease in neutrophils adhesion 22 and increased NO production, which combine with superoxide anions blocking their effects. 23 In experimental pancreatitis in rats, it was demonstrated that HOT promotes an elevation in levels of super oxide dismutase (SOD) with metabolization of oxidant radicals.…”

Section: Discussionmentioning

confidence: 99%

“…Until recently most studies about cisplatin ototoxicity evaluated cochlea functional alterations through action potential, somation potential and cochlear microphonics or through Brainstem Evoked Auditory Potentials (BAEP 11,[14][15][16][17][18][19] . Several of these drugs have in vivo collateral effects and little is known about a possible interference action on the anti-neoplastic activity of cisplatin.…”

Section: Discussionmentioning

confidence: 99%

“…Drugs like fosfomycin, sulfur compounds, sodium thiosulfate, diethyl carbamate, adrenocorticotropic hormone (ACTH) and derivatives, 4-methylthiobenzoic acid, lipoic acid, glutathione and esters, metionine, alpha-melanocytic stimulating hormone (melatonin) and anti-oxidants like ginkgo biloba extracts, all anti-free radicals, were effectively tested. 11,[14][15][16][17][18][19][20] Hyperbaric oxygen therapy (HOT), having an important anti-oxidant activity is being increasingly used and it is based on inhalation of 100% oxygen under pressures above 1 atm. It is precisely indicated as a stimulant of wound healing and cellular growth.…”

Section: Introductionmentioning

confidence: 99%

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The role of hyperbaric oxygen therapy (HOT) as an otoprotection agent against cisplatin ototoxicity

et al. 2008

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Purpose: Hyperbaric oxygen therapy (HOT) consists of intermittent inhalations of 100% oxygen at a pressure higher than 1 atm. It is an important adjuvant therapy in pathological processes like soft tissue infections, radiation injury, gas gangrene, osteomyelitis and decompressive diseases. Cisplatin, a potent antineoplastic drug, widely used in cancer therapy is highly ototoxic causing bilateral, irreversible damage to the hearing of high frequency sounds (4-8 KHz). Objective:This experimental study conducted at the Faculty of Medicine of Ribeirao Preto, University of Sao Paulo aims to evaluate Hyperbaric Oxygen Therapy as an otoprotection agent against drug toxicity. Methods: Albino guinea pigs were divided into two groups: in Group A, 5 animals (10 cochlea) received cisplatin, i. p., 8.0 mg/kg/day during three days and afterwards were submitted to HOT; in Group B, 3 animals (6cochlea) received cisplatin, i. p. 8.0mg/kg/day during three days. Guinea pigs were evaluated by acoustic otoemissions (AOE) and scanning electron microscopy (SEM). Results: Group B animals showed loss of auditory functions as measured by AOE and distorted outer hair cells by SEM. In Group A, outer hair cells shown by SEM images were mostly preserved. Conclusion: It is presumed that Hyperbaric Oxygen Therapy has a protector effect against cisplatin ototoxicity.Key words: Hyperbaric Oxygen Therapy. Ototoxicity. Otoprotection. Cisplatin. RESUMOIntrodução: A Oxigenoterapia hiperbárica (OHB) envolve a inalação de oxigênio a 100% sob uma pressão maior que 1 atm. E um importante modo de terapia adjuvante para processos patológicos, tais como: infecção de partes moles, lesões actínicas, gangrena gasosa, osteomielite e doença descompressiva. A cisplatina e uma potente droga antineoplásica largamente utilizada para o tratamento de câncer. A ototoxicidade e um importante efeito colateral desta droga, causando dano irreversível, bilateral, na capacidade de ouvir sons de alta freqüência (4 -8 KHz). Este estudo experimental, realizado na Faculdade de Medicina de Ribeirao Preto da Universidade de São Paulo nos anos de 2005 e 2006. Objetivo: Avaliar o papel da Oxigenoterapia Hiperbárica como agente otoprotector contra a toxicidade de drogas. Métodos: Cobaias albinas divididas em 2 grupos Grupo A: com 5 cobaias (10 cócleas) que receberam cisplatina 8,0 mg/kg/dia, via intraperitoneal por 3 dias, submetidas posteriormente a OHB. Grupo B: com 3 cobaias (6 cócleas) que receberam cisplatina 8,0 mg/kg/dia, via intraperitoneal por 3 dias. As cobaias foram avaliadas através de otoemissões acústicas (OEA) e por microscopia eletrônica de varredura (MEV). Resultados: Encontramos no grupo B perda da função auditiva medida pela OEA e distorção das células ciliares externas a MEV. No grupo A, a MEV as células ciliares externas foram preservadas em sua grande maioria. Conclusão: Assim podemos supor que a Oxigenoterapia Hiperbárica tem um efeito otoprotetor contra a ototoxicidade induzida pela cisplatina.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The role of hyperbaric oxygen therapy (HOT) as an otoprotection agent against cisplatin ototoxicity

et al. 2008

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“…On a recent search of the specific literature there were less than ten references which appeared upon a cross-search using "nephrotoxicity" and Ginkgo biloba as keywords. All papers showed that this herb is beneficial and protects the kidney from nephrotoxic substances such as uranium (Yapar et al, 2010), adriamycin (AbdEllah & Mariee, 2007), cisplatin (Fukaya & Kanno, 1999;Gulec et al, 2006), gentamicine (Naidu et al, 2000), and vancomycin (Celik et al, 2005). On the other hand, some herbal medications have potentially harmful side effects, as well as adverse interactions with prescription drugs (Qato, 2008).…”

Section: Discussionmentioning

confidence: 99%

Therapeutic dose of Ginkgo biloba extract 761 may alter the urine excretion of Wistar rats

Dalmacio¹,

Campos²,

Carvalho³

et al. 2012

Rev. bras. farmacogn.

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Abstract:Wistar rats (n=20) were divided in two groups: G1 received 2 mg/kg of GBE (Ginkgo biloba extract 761), whereas G2 received the same volume of a sodium chloride solution (0.9%), both for 10 days. After a 7-day interval, the treatment was repeated for 8 days. Urine volume and food and water intake were measured daily during this protocol. Histological assessments were performed. No significant difference (p>0.05) was observed in food and water intake of animals during treatment with GBE. Animals who received GBE had a smaller urine volume and increase of weight with a significance difference (p<0.05) during the first and second exposure period.

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“…10,[27][28][29][30][31][32][33][34] Diversas drogas têm sido testadas com o objetivo de otoproteção como o tiossulfato de sódio, o dietilcarbamato, o ACTH e derivados, o ácido 4-metilthiobenzóico, o ácido lipóico, o glutation e seus ésteres, metionina, procaína, hormônio estimulador alfa-melanocítico (melatonina), antioxidantes como a ginkgo biloba e fosfomicina e compostos sulfurados. 3,5,6,9,[35][36][37][38][39][40][41][42][43][44][45][46][47] Trabalho recente de tese de mestrado da FMRP -USP mostrou que baixas doses de gentamicina levam a um efeito protetor estrutural para as células ciliadas externas, que mantém suas características normais à microscopia eletrônica de varredura quando se aplicam, posteriormente, doses elevadas desta droga, sugerindo assim, que existe um mecanismo de autodefesa -adaptação intra-coclear às agressões induzidas pelas drogas ototóxicas. [48][49][50][51] O objetivo da investigação é verificar se existe o efeito otoprotetor de drogas que já são utilizadas na prática clínica com outras finalidades como o extrato de ginkgo biloba, aos danos cocleares causados pela cisplatina, utilizando doses de cisplatina sabidamente ototóxicas, lesivas às células ciliadas externas, avaliando-se as alterações anatômicas através da microscopia eletrônica de superfície, e avaliação funcional por emissões otoacústicas -produtos de distorção.…”

Section: Introductionunclassified

Ototoxicidade da cisplatina e otoproteção pelo extrato de ginkgo biloba às células ciliadas externas: estudo anatômico e eletrofisiológico

Hyppolito¹,

Oliveira²,

Rossato³

et al. 2003

Rev. Bras. Otorrinolaringol.

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A Cisplatina é uma potente droga antineoplásica, largamente utilizada para o tratamento do câncer, tanto em adultos quanto em crianças. Dentre seus efeitos colaterais, a ototoxicidade se apresenta como um dos mais importantes e leva à perda auditiva irreversível, bilateral, para as altas freqüências (4KHz#8KHz). Estudos têm tentado identificar drogas que, associadas à cisplatina possam atuar como otoprotetores. Sabe-se que o mecanismo da ototoxicidade pela cisplatina está relacionado a alterações nos mecanismos antioxidantes das células ciliadas, principalmente as células ciliadas externas da cóclea. Objetivo: Nossa proposta foi de avaliar através de emissões otoacústicas, por produtos de distorção (EOAPD) e por microscopia eletrônica de superfície (ME), a ação do extrato de ginkgo biloba (EGB 761), que tem conhecida ação antioxidante, como possível otoprotetor, utilizando como modelo experimental cobaias albinas. Forma de estudo: Experimental. Material e método: Observamos EOAPD presentes pré e pós tratamento no grupo EGB (100 mg/Kg/dia via oral) e 90 minutos após cisplatina (80 mg/Kg/dia via intraperitoneal) por 8 dias. Resultado: Houve também manutenção da arquitetura ciliar nas células ciliadas externas em todas as espiras da cóclea, enquanto que no grupo tratado somente com cisplatina (80 mg/ Kg/dia via intraperitoneal) por 8 dias, houve desaparecimento das EOAPD pós tratamento, com desaparecimento dos cilios das células ciliadas externas e distorção na arquitetura dos cílios remanescentes à ME. Conclusão: Concluímos que a EGB, por sua ação antioxidante, atua como fator otoprotetor à ototoxicidade pela cisplatina, devendo ser testada tal ação na prática clínica em pacientes que utilizam a cisplatina, pois o uso do EGB está extremamente difundido no tratamento de diferentes doenças. ci splatin is an antineoplastic drug for cancer treatment in children and adults. The side effects of cisplatin ototoxycity are important with irreversible auditory and bilateral damage to high frequencies (4kHz -8 kHz). Reports recognize some drugs that are associated with cisplatin to obtain an otoprotector effect. The ototoxycity mechanisms of cisplatin are related to injury of conduct the hair cell oxidation mechanism, with particular injury to outer hair cells. Aim: We intend to studies using otoacoustic emissions -distortion products (DPOEA) and scanning microscopy to verify the action of ginkgo biloba (GBE-761) that has well known antoxidizing characteristics, that can function like otoprotector effects. Study design: Experimental. Material and method: We use an experimental guinea pig model. We found DPOEA positive before and after treatment in the GBE group (100 mg/ Kg/ day -oral) and after 90 minutes cisplatin (8,0 mg/ Kg/ day -intraperitoneal -8 consecutive days). Results: The normal cilium architecture of outer hair cells was supported in all cochlear spirals and in the group treated only with cisplatin (8,0 mg/ Kg/ day -intraperitoneal -8 consecutive days), the DPOEA was not present and strong injury to cilium of out...

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Experimental Studies of the Protective Effect of Ginkgo Biloba Extract(GBE) on Cisplatin-induced Toxicity in Rats.

Cited by 32 publications

References 24 publications

The role of hyperbaric oxygen therapy (HOT) as an otoprotection agent against cisplatin ototoxicity

The role of hyperbaric oxygen therapy (HOT) as an otoprotection agent against cisplatin ototoxicity

Therapeutic dose of Ginkgo biloba extract 761 may alter the urine excretion of Wistar rats

Ototoxicidade da cisplatina e otoproteção pelo extrato de ginkgo biloba às células ciliadas externas: estudo anatômico e eletrofisiológico

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