Experimental Studies of Natural Purification in Polluted Waters: VII. The Selection of a Dilution Water for Bacteriological Examinations

Butterfield, C. T.

doi:10.2307/4580814

Cited by 10 publications

(2 citation statements)

References 5 publications

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“…However, we did not assess whether any destruction of polymyxin or sulfadiazine occurs during autoclaving, which might affect the selectivity of the medium. Butterfield's phosphate buffer (Butterfield, 1933 ;American Public Health Association, 1960) is being widely used as a diluent for C. perfringens cultures. It was also used by Angelotti et al (1962).…”

Section: Discussionmentioning

confidence: 99%

“…The cultures were diluted in Butterfield's phosphate buffer (Butterfield, 1933 ;American Public Health Assosociation., 1960) or in 0.1% peptone, and duplicate samples of 1.0 ml were plated. The solid media were Nutrient agar (Difco), Liver-veal agar (Difco), and SPS agar which was prepared in this laboratory according to the method of Angelotti et al (1962) or obtained from Baltimore Biological Laboratories (BBL) and from Difco Laboratories.…”

Section: Growth and Cell Enumerationmentioning

confidence: 99%

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Variations in Recovery of Clostridium perfringens on Commercial Sulfite‐Polymyxin‐Sulfadiazine (SPS) Agar

Hauschild¹,

Erdman²,

Hilsheimer³

et al. 1967

Journal of Food Science

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SUMMARY— Liquid cultures of several strains of Clostridium perfringens type A were diluted with phosphate buffer and enumerated on laboratory‐prepared sulfite‐polymyxin‐sulfa‐diazine (SPS) agar, four lots of commercial SPS agar, and on non‐selective media. Recoveries on laboratory‐prepared SPS agar and on one lot of commercial SPS agar were the same as on non‐selective media. Recoveries on three commercial lots of SPS agar were significantly lower. Replacement of phosphate buffer with 0.1% peptone allowed quantitative recoveries of C. perfringens on three out of four lots of commercial SPS agar, irrespective of whether the cells were grown in liquid cultures or on meat and meat products.

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Section: Discussionmentioning

confidence: 99%

Section: Growth and Cell Enumerationmentioning

confidence: 99%

Variations in Recovery of Clostridium perfringens on Commercial Sulfite‐Polymyxin‐Sulfadiazine (SPS) Agar

Hauschild¹,

Erdman²,

Hilsheimer³

et al. 1967

Journal of Food Science

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Survivor Curves of Bacterial Spores Heated in Parenteral Solutions

Pflug

Smith

1977

Spore Research 1973

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Effect of phosphate buffer concentration on the heat resistance of Bacillus stearothermophilus spores suspended in parenteral solutions

Gauthier¹,

Smith²,

Pflug³

1978

Appl Environ Microbiol

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The effect of various quantities of Butterfield phosphate buffer added to four parenteral solutions on the survival of Bacillus stearothermophilus spores heated at 121 degrees C was determined. The effect of the addition of phosphate buffer on spore survival varied with the parenteral solution. Spore survival was increased or decreased, depending upon the composition of the parenteral solution and the buffer concentration. The results obtained in these experiments attest to the fact that environmental factors, including the type of ions present and ionic concentration, affect the heat destruction rate of B. stearothermophilus spores. Therefore, the sterilization requirements of a product such as a parenteral solution may be affected by small changes in formulation.

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Experimental Studies of Natural Purification in Polluted Waters: VII. The Selection of a Dilution Water for Bacteriological Examinations

Cited by 10 publications

References 5 publications

Variations in Recovery of Clostridium perfringens on Commercial Sulfite‐Polymyxin‐Sulfadiazine (SPS) Agar

Variations in Recovery of Clostridium perfringens on Commercial Sulfite‐Polymyxin‐Sulfadiazine (SPS) Agar

Survivor Curves of Bacterial Spores Heated in Parenteral Solutions

Effect of phosphate buffer concentration on the heat resistance of Bacillus stearothermophilus spores suspended in parenteral solutions

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