Experimental studies of hydroxyapatite by Raman spectroscopy

Тимченко, П. Е.; Timchenko, E. V.; Pisareva, E. V.; Vlasov, M. Yu.; Волова, Л. Т.; Frolov, O. O.; Kalimullina, A. R.

doi:10.1364/jot.85.000130

Cited by 38 publications

(31 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Both the FT-IR and Raman spectroscopic data, as tabulated in Tables 1 and 2tbl2, respectively, reveal that all the characteristic band positions, as well as the Raman shis, are in good agreement with those observed in previous studies. 19,31 Moreover, the presence of CaCO 3 in the UV-synthesized Hap is also supported by the peaks, as evidenced for the CO 3 2À group at band positions 877 and 1418 cm À1 in the FT-IR spectrum. Further conrmation of the CO 3 2À group was provided by the Raman spectroscopic data, in which the Raman shi for the CO 3 2À group was observed at 1070 cm À1 .…”

Section: Characterization Of the Uv-sensitized Hapmentioning

confidence: 82%

UV-assisted synthesis of hydroxyapatite from eggshells at ambient temperature: cytotoxicity, drug delivery and bioactivity

et al. 2021

View full text Add to dashboard Cite

show abstract

Section: Characterization Of the Uv-sensitized Hapmentioning

confidence: 82%

UV-assisted synthesis of hydroxyapatite from eggshells at ambient temperature: cytotoxicity, drug delivery and bioactivity

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Further, the existence of the normalCO3 2− group was affixed by the Raman spectroscopic data (figure 14) having the Raman shift at 1065 cm −1 [46,47]. A common feature of symbolic Raman spectrum of B-type carbonated apatite (i.e.…”

Section: Resultsmentioning

confidence: 99%

Coupled effect of particle size of the source materials and calcination temperature on the direct synthesis of hydroxyapatite

et al. 2021

View full text Add to dashboard Cite

We report the effect of controlled particle size (obtained by using 80, 100, 120, 140 and 200 mesh) of the source materials on the synthesis of a well-known biomaterial, hydroxyapatite (Hap). In addition to this, we have also mapped the consequence of applied temperature (700°C, 800°C and 900°C) on the crystallographic properties and phase composition of the obtained Hap. Nevertheless, although with Hap, in each case, β-tricalcium phosphate (β-TCP) was registered as the secondary phase the ANOVA test revealed that the results of the crystallographic parameters are significantly different for the applied sintering temperature 700°C and 800°C ( p < 0.05), while the data obtained for calcination temperature 800°C are not significantly different from that acquired at 900°C ( p > 0.05). Fourier transform infrared spectrophotometer data ensured that, irrespective of mesh size and calcination temperature, the synthesized Hap samples were of carbonated apatite with B-type substitution. Interestingly, for all cases, the % of carbonate content was below the maximum limit (8%) of the CO 3 2 − ion present in bone tissue hydroxyapatite.

show abstract

“…Distribution in control and ADSC samples was shown by peak area, compared to visible microscopic image. For differentiation of compound changes induced by surroundings peak width was introduced, indicating shift to higher wavenumbers, which was the result of highly crystalline structure of HA [ 39 ]. Proven formation of a new layer of HA in cell-seeded samples demonstrates surface biomineralisation resulting in a good bioactivity of the scaffold.…”

Section: Discussionmentioning

confidence: 99%

Application of Raman Spectroscopic Imaging to Assess the Structural Changes at Cell-Scaffold Interface

Kalisz

Przekora

Kazimierczak

et al. 2021

IJMS

View full text Add to dashboard Cite

Raman spectroscopic imaging and mapping were applied to characterise three-compound ceramic composite biomaterial consisting of chitosan, β-1,3-d-glucan (curdlan) and hydroxyapatite (HA) developed as a bone tissue engineering product (TEP). In this rapidly advancing domain of medical science, the urge for quick, reliable and specific method for products evaluation and tissue–implant interaction, in this case bone formation process, is constantly present. Two types of stem cells, adipose-derived stem cells (ADSCs) and bone marrow-derived stem cells (BMDSCs), were cultured on composite surface. Raman spectroscopic imaging provided advantageous information on molecular differences and spatial distribution of compounds within and between the cell-seeded and untreated samples at a microscopic level. With the use of this, it was possible to confirm composite biocompatibility and bioactivity in vitro. Deposition of HA and changes in its crystallinity along with protein adsorption proved new bone tissue formation in both mesenchymal stem cell samples, where the cells proliferated, differentiated and produced biomineralised extracellular matrix (ECM). The usefulness of spectroscopic Raman imaging was confirmed in tissue engineering in terms of both the organic and inorganic components considering composite–cells interaction.

show abstract

Experimental studies of hydroxyapatite by Raman spectroscopy

Cited by 38 publications

References 0 publications

UV-assisted synthesis of hydroxyapatite from eggshells at ambient temperature: cytotoxicity, drug delivery and bioactivity

UV-assisted synthesis of hydroxyapatite from eggshells at ambient temperature: cytotoxicity, drug delivery and bioactivity

Coupled effect of particle size of the source materials and calcination temperature on the direct synthesis of hydroxyapatite

Application of Raman Spectroscopic Imaging to Assess the Structural Changes at Cell-Scaffold Interface

Contact Info

Product

Resources

About