Experimental setup of field lysimeters for studying effects of elevated ozone and below-ground pathogen infection on a plant-soil-system of juvenile beech (Fagus sylvatica L.)

Winkler, Jana Barbro; Lang, Hans; Graf, Wolfgang; Reth, Sascha; Munch, Jean Charles

doi:10.1007/s11104-009-9936-x

Cited by 17 publications

(17 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A detailed description of the lysimeter experiment at the Lysimeter Station of the Helmholtz Center Munich, German Research Center for Environmental Health is given by Schloter et al (2005), Pritsch et al (2008), and Winkler et al (2009b).…”

Section: Methodsmentioning

confidence: 99%

Structure and activities of ectomycorrhizal and microbial communities in the rhizosphere of Fagus sylvatica under ozone and pathogen stress in a lysimeter study

et al. 2009

View full text Add to dashboard Cite

The aim was to study the influence of abiotic (elevated ozone) or biotic stress (Phytophthora citricola) or their combination on soil biological components and processes in the rhizosphere of young beech trees. Ectomycorrhizal and overall microbial community composition was studied at two soil depths in a lysimeter experiment with 7 year old trees of Fagus sylvatica. As a functional parameter, potential enzyme activities were measured in mycorrhizosphere soil and on excised mycorrhizal tips. The degree of mycorrhization, structure and potential enzymatic activities of mycorrhizal communities were only slightly influenced by treatments. Soil enzyme activities were depressed under elevated ozone and stimulated by P. citricola under ambient but not under elevated ozone. Overall microbial community composition (PLFA) and ectomycorrhizal diversity changed with depth. PLFA analyses not only suggested a reaction of the microbial community to elevated ozone but also indicated an increase in plant stress related components. No influence of the biotic stress on ectomycorrhizal or overall microbial community structure was detected. Changes in the mycorrhizosphere community structure and function due to ozone may be explained by the quality of plant derived carbon.

show abstract

Section: Methodsmentioning

confidence: 99%

Structure and activities of ectomycorrhizal and microbial communities in the rhizosphere of Fagus sylvatica under ozone and pathogen stress in a lysimeter study

et al. 2009

View full text Add to dashboard Cite

show abstract

“…9°C (2003, 2006). The experimental setup of the forest soil lysimeters is described in detail by Winkler et al (2009). In brief, eight lysimeters, each with a surface area of 1 m 2 and a depth of 2 m, were filled in 1999 with soil material (Haplic Cambisol dystric) from the forest site "Högl-wald" (48°18' N 11°05' E, 540 m) near Augsburg (Bavaria, Germany).…”

Section: Experimental Designmentioning

confidence: 99%

Initial differentiation of vertical soil organic matter distribution and composition under juvenile beech (Fagus sylvatica L.) trees

Mueller¹,

Brüggemann²,

Pritsch

et al. 2009

Plant Soil

Self Cite

View full text Add to dashboard Cite

In a lysimeter experiment with juvenile beech trees (Fagus sylvatica L.) we studied the development of depth gradients of soil organic matter (SOM) composition and distribution after soil disturbance. The sampling scheme applied to the given soil layers (0-2 cm, 2-5 cm, 5-10 cm and 10-20 cm) was crucial to study the subtle reformation of SOM properties with depth in the artificially filled lysimeters. Due to the combination of physical SOM fractionation with the application of 15 N-labelled beech litter and 13 C-CPMAS NMR spectroscopy we were able to obtain a detailed view on vertical differentiation of SOM properties. Four years after soil disturbance a significant decrease of the mass of particulate OM (POM) with depth could be found. A clear depth distribution was also shown for carbon (C) and nitrogen (N) within the SOM fractions related to bulk soil. The mineral fractions <63 µm clearly dominated C storage (between 47 to 60% of bulk soil C) and N storage (between 68 to 86% of bulk soil N). A drastic increase in aliphatic C structures concomitant to decreasing O/N-alkyl C was detected with depth, increasing from free POM to occluded POM. Only a slight depth gradient was observed for 13 C but a clear vertical incorporation of 15 N from the applied labelled beech litter was demonstrated probably resulting from faunal and fungal incorporation. We clearly demonstrated a significant reformation of a SOM depth profile within a very short time of soil evolution. One important finding of this study is that especially in soils Plant Soil (

show abstract

“…The experimental setup has been described in detail by Winkler et al (2009) as well as by Schloter et al (2005) and Pritsch et al (2008). In brief, the experiment was conducted at the field lysimeter station of the Helmholtz Zentrum München (48°13′ N 11°36′ E, 490 m altitude).…”

Section: Study Site and Treatmentsmentioning

confidence: 99%

“…A detailed description of the O 3 enrichment system using free-air fumigation methodology (cf. Karnosky et al 2007b) is given by Winkler et al (2009). In the first year 2003, O 3 fumigation started in June and was continued during daytime until December.…”

Section: Study Site and Treatmentsmentioning

confidence: 99%

“…Under greenhouse conditions, beech seedlings sustained severe damage after P. citricola infection (Fleischmann et al 2002. The inoculation of the trees under otherwise natural conditions was only possible using the lysimeters as they provide containment that avoids an infection of the control trees (Fleischmann et al 2009;Winkler et al 2009). …”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Do chronic aboveground O3 exposure and belowground pathogen stress affect growth and belowground biomass partitioning of juvenile beech trees (Fagus sylvatica L.)?

et al. 2009

Self Cite

View full text Add to dashboard Cite

The impact of chronic free air ozone (O 3 ) exposure and belowground pathogen stress on growth and total biomass development of young beech trees (Fagus sylvatica L.) was investigated in a lysimeter study. Plants were growing during four years under ambient or elevated atmospheric O 3 concentrations. Additionally, in the last vegetation period the root rot pathogen Phytophthora citricola was introduced to study the interaction of ozone exposure and pathogen stress in the soil-plant system. A complete harvest at the end of the experiment enabled for the first time the assessment of fine and coarse root biomass of individual trees with a high vertical resolution down to two meter depth. Plant growth was significantly reduced by elevated ozone but not affected by P. citricola. Biomass partitioning between fine and coarse roots as well as vertical root distribution were significantly affected by both factors, whereas changes in root/shoot biomass ratio were not observed.

show abstract

Experimental setup of field lysimeters for studying effects of elevated ozone and below-ground pathogen infection on a plant-soil-system of juvenile beech (Fagus sylvatica L.)

Abstract: An experiment, focusing on the effects of chronically enhanced O 3 regimes on young beech (Fagus sylvatica) and on the microbial rhizosphere community structure, was conducted from

Cited by 17 publications

References 49 publications

Structure and activities of ectomycorrhizal and microbial communities in the rhizosphere of Fagus sylvatica under ozone and pathogen stress in a lysimeter study

Structure and activities of ectomycorrhizal and microbial communities in the rhizosphere of Fagus sylvatica under ozone and pathogen stress in a lysimeter study

Initial differentiation of vertical soil organic matter distribution and composition under juvenile beech (Fagus sylvatica L.) trees

Do chronic aboveground O3 exposure and belowground pathogen stress affect growth and belowground biomass partitioning of juvenile beech trees (Fagus sylvatica L.)?

Contact Info

Product

Resources

About