Experimental Second Passage of Chronic Wasting Disease (CWDmule deer) Agent to Cattle

Hamir, Amir N.; Kunkle, Robert A.; Miller, Janice M.; Greenlee, Justin J.; Richt, Jüergen A.

doi:10.1016/j.jcpa.2005.07.001

Cited by 47 publications

(51 citation statements)

References 15 publications

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“…[22][23][24] However, intracerebral CWD inoculation caused prion infection in voles, hamsters, ferrets, sheep, cats, mink, and cattle, with variable attack rates. [25][26][27][28][29][30][31][32][33] Wild type mice and raccoons resisted CWD prion infection (Table 1). 16,34,35 An extensive study of CWD susceptibility in transgenic mice expressing ovine and bovine PrP C revealed no mice with prion disease, supporting the strong barrier to CWD infection observed in sheep and cattle.…”

Section: Cross-species Cwd Prion Transmissionmentioning

confidence: 99%

Cross-species transmission of CWD prions

Kurt

Sigurdson

2016

Prion

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Section: Cross-species Cwd Prion Transmissionmentioning

confidence: 99%

Cross-species transmission of CWD prions

Kurt

Sigurdson

2016

Prion

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“…Past experience at NADC has indicated that these antibodies will recognize PRNP sequences in livestock species in which experimental TSEs have been reported. 3,[5][6][7][8][9]11 For detecting PrP res by WB, a method described previously was used on frozen brain (caudal medulla) tissue. 16 The antibody used in this technique was 6H4.…”

Section: Necropsy and Samplesmentioning

confidence: 99%

Transmission of Chronic Wasting Disease of Mule Deer to Suffolk Sheep following Intracerebral Inoculation

et al. 2006

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Abstract. To determine the transmissibility of chronic wasting disease (CWD) to sheep, 8 Suffolk lambs of various prion protein genotypes (4 ARQ/ARR, 3 ARQ/ARQ, 1 ARQ/VRQ at codons 136, 154, and 171, respectively) were inoculated intracerebrally with brain suspension from mule deer with CWD (CWD md ). Two other lambs were kept as noninoculated controls. Within 36 months postinoculation (MPI), 2 inoculated animals became sick and were euthanized. Only 1 sheep (euthanized at 35 MPI) showed clinical signs that were consistent with those described for scrapie. Microscopic lesions of spongiform encephalopathy (SE) were only seen in this sheep, and its tissues were determined to be positive for the abnormal prion protein (PrP res ) by immunohistochemistry and Western blot. Three other inoculated sheep were euthanized (36 to 60 MPI) because of conditions unrelated to TSE. The 3 remaining inoculated sheep and the 2 control sheep did not have clinical signs of disease at the termination of the study (72 MPI) and were euthanized. Of the 3 remaining inoculated sheep, 1 was found to have SE, and its tissues were positive for PrP res . The sheep with clinical prion disease (euthanized at 35 MPI) was of the heterozygous genotype (ARQ/VRQ), and the sheep with subclinical disease (euthanized at 72 MPH) was of the homozygous ARQ/ARQ genotype. These findings demonstrate that transmission of the CWD md agent to sheep via the intracerebral route is possible. Interestingly, the host genotype may play a notable part in successful transmission and incubation period of CWD md .

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“…In addition, Eurasian red deer (Cervus elaphus) (16), Eurasian fallow deer (Dama dama) (17), Asian muntjac deer (Muntiacus reevesi) (18), and reindeer (19,20) have been shown to be susceptible to CWD following experimental inoculation. CWD has been experimentally transmitted to noncervid species, including sheep (21), cattle (22)(23)(24)(25), domestic cats (26,27), ferrets (28,29), nonhuman primates (30)(31)(32), and laboratory rodents (reviewed in reference 33).…”

mentioning

confidence: 99%

Experimental Transmission of the Chronic Wasting Disease Agent to Swine after Oral or Intracranial Inoculation

Moore

Greenlee

Kondru

et al. 2017

J Virol

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Chronic wasting disease (CWD) is a naturally occurring, fatal neurodegenerative disease of cervids. The potential for swine to serve as hosts for the agent of CWD is unknown. The purpose of this study was to investigate the susceptibility of swine to the CWD agent following experimental oral or intracranial inoculation. Crossbred piglets were assigned to three groups, intracranially inoculated (n ϭ 20), orally inoculated (n ϭ 19), and noninoculated (n ϭ 9). At approximately the age at which commercial pigs reach market weight, half of the pigs in each group were culled ("market weight" groups). The remaining pigs ("aged" groups) were allowed to incubate for up to 73 months postinoculation (mpi). Tissues collected at necropsy were examined for disease-associated prion protein (PrP Sc ) by Western blotting (WB), antigen capture enzyme immunoassay (EIA), immunohistochemistry (IHC), and in vitro real-time quaking-induced conversion (RT-QuIC). Brain samples from selected pigs were also bioassayed in mice expressing porcine prion protein. Four intracranially inoculated aged pigs and one orally inoculated aged pig were positive by EIA, IHC, and/or WB. By RT-QuIC, PrP Sc was detected in lymphoid and/or brain tissue from one or more pigs in each inoculated group. The bioassay was positive in four out of five pigs assayed. This study demonstrates that pigs can support low-level amplification of CWD prions, although the species barrier to CWD infection is relatively high. However, detection of infectivity in orally inoculated pigs with a mouse bioassay raises the possibility that naturally exposed pigs could act as a reservoir of CWD infectivity. IMPORTANCEWe challenged domestic swine with the chronic wasting disease agent by inoculation directly into the brain (intracranially) or by oral gavage (orally). Disease-associated prion protein (PrP Sc ) was detected in brain and lymphoid tissues from intracranially and orally inoculated pigs as early as 8 months of age (6 months postinoculation). Only one pig developed clinical neurologic signs suggestive of prion disease. The amount of PrP Sc in the brains and lymphoid tissues of positive pigs was small, especially in orally inoculated pigs. Regardless, positive results obtained with orally inoculated pigs suggest that it may be possible for swine to serve as a reservoir for prion disease under natural conditions.

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Experimental Second Passage of Chronic Wasting Disease (CWDmule deer) Agent to Cattle

Cited by 47 publications

References 15 publications

Cross-species transmission of CWD prions

Cross-species transmission of CWD prions

Transmission of Chronic Wasting Disease of Mule Deer to Suffolk Sheep following Intracerebral Inoculation

Experimental Transmission of the Chronic Wasting Disease Agent to Swine after Oral or Intracranial Inoculation

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