Experimental research on end-to-side anastomosis of peripheral nerves and effect of FK506 on end-to-side anastomosis

Abstract

Abstract:Objective: To study the effects of end-to-side anastomosis of initially-denatured nerves at different times. Method: 60 male Wistar albino rats were used to fabricate animal models for the experiment on end-to-side anastomosis of peripheral nerves and 50 female Wistar albino rats were used to fabricate animal models for the experiment on the effect of FK506 on end-to-side anastomosis. Bilateral common peroneal nerve, tibialis anterior muscle electrophysiological and histological examinations, tibialis anterior muscle wet muscle weight determination, and motor end plate examination were performed 3 months after operation.Results: All recovery rates of action potential, single muscle contraction force and tetanic contraction force of the FK506 experimental group are signifi cantly higher than those of the control group and the sectional area of muscle fi ber is also higher than that of the control group of normal saline. Conclusion:The best time for end-to-side anastomosis of nerves should be controlled within 2 weeks and the effect of end-to-side anastomosis of nerves will gradually become unsatisfactory. FK506 plays a role in promoting functional rehabilitation following nerve end-to-side anastomosis (Tab. 7, Fig. 4, Ref. 31). Text in PDF www.elis.sk.

A histomorphometric study of unmyelinated fibers of the fibular nerve in Wistar rats

“…The use of the fibular nerve in studies of nerve regeneration was boosted after the reintroduction of the end-to-side neurorrhaphy 1,2,3,4,5,6,7,8,9,10,11,23,13 . We were not able to find many references to the unmyelinated axons of the fibular nerve in these and other recent studies.…”

Nerve Wrap for Local Delivery of FK506/Tacrolimus Accelerates Nerve Regeneration

“…Experimentally, FK506 significantly increases both the rate of axonal regeneration and myelinated nerve fiber density following peripheral nerve transection and repair [23][24][25]. Overall, systemic FK506 administration accelerates nerve recovery and improves outcomes after nerve injury and repair [26][27][28][29][30][31]. However, systemic immunosuppression is responsible for numerous poorly tolerated, global side effects that can lead to organ failure [32][33][34][35][36][37].…”