Experimental rat model of chronic cerebral hypoperfusion–reperfusion mimicking normal perfusion pressure breakthrough phenomenon

Revuelta, Juan Manuel; Zamarrón, A; Fortes, José; Rodríguez-Boto, G.; Vaquero, Jesús; Gutiérrez-González, Raquel

doi:10.1016/j.neucir.2019.11.002

Cited by 2 publications

(7 citation statements)

References 17 publications

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“…All animals (study and control) showed a similar pattern regarding weight during the research development. According to the previous model 14 , weight significantly decreased at an early stage, with a subsequent recovery up to basal situation on day 60 (Table 2 ).…”

Section: Resultsmentioning

confidence: 61%

“…The research was based on a previous experimental model 14 . The study was approved by the Animal Care and Use Committee (CEBA) of “Puerta de Hierro” University Hospital (reference CEBA 016/2012; date April 16th 2012).…”

Section: Methodsmentioning

confidence: 99%

“…Minor procedure involved the placement of a sensor for ICP registration in the left-brain hemisphere by means of a post-coronal trepanation and durotomy. Major procedure entailed the creation of a left arteriovenous fistula (AVF) by means of an end-to-side anastomosis between the external jugular vein and the common carotid artery, similar to the one described in the previous model 14 . Bilateral external carotid arteries were also ligated.…”

Section: Methodsmentioning

confidence: 99%

“…At the end of the experiment (day 60) and under general anaesthesia AVF was ligated during 60 min in all animals in order to resemble NPPB phenomenon 14 . Thereafter, and with the aim of evaluating BBB integrity, three animals of each group were perfused with fluorescein sodium (10% NaFl—0.6 mL/kg) via transcarotid injection.…”

Section: Methodsmentioning

confidence: 99%

“…At this same point, and after AVF closure for 60 min, the remaining three animals in each group underwent transcardial perfusion with 4% paraformaldehyde solution in phosphate buffered and were euthanized following a previous model 14 . Brain samples were collected and fixed in formalin.…”

Section: Methodsmentioning

confidence: 99%

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Neuroprotective effect of indomethacin in normal perfusion pressure breakthrough phenomenon

Revuelta

Zamarrón

Fortes

et al. 2020

Sci Rep

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Loss of cerebral autoregulation in normal perfusion pressure breakthrough (NPPB) phenomenon has been reported in other Central Nervous System diseases such as neonatal intraventricular haemorrhage. Several studies have demonstrated that low-dose indomethacin prevents this latter condition. A previous rat model was used to resemble NPPB phenomenon. Study animals were distributed in 4 groups that received 3 doses of indomethacin at different concentrations prior to fistula occlusion 60 days after its creation. Control animals received saline solution. Intracranial pressure (ICP) increased in all groups following fistula creation, whereas mean arterial pressure (MAP) and cerebral perfusion pressure (CPP) decreased as a manifestation of cerebral hypoperfusion and intracranial hypertension. The administration of indomethacin was associated with raised MAP and CPP, as well as decreased ICP. Sodium fluorescein extravasation was slight in study animals when comparing with control ones. Histological analysis evidenced diffuse ischaemic changes with signs of neuronal apoptosis in all brain layers in control animals. These findings were only focal and slight in study animals. The results suggest the usefulness of indomethacin to revert, at least partially, the haemodynamic effects of NPPB phenomenon in this experimental model, as well as to reduce BBB disruption and histological ischemia observed in absence of indomethacin.

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Section: Resultsmentioning

confidence: 61%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Neuroprotective effect of indomethacin in normal perfusion pressure breakthrough phenomenon

Revuelta

Zamarrón

Fortes

et al. 2020

Sci Rep

Self Cite

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Development of a Method for Quantitative Evaluation of Facial Swelling in a Rat Model of Cerebral Ischemia by Facial Image Processing

Liu

Huang²,

et al. 2022

Front. Med.

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A quantitative method for the evaluation of facial swelling in rats with middle cerebral artery occlusion (MCAO) was established using a mathematical method for the first time. The rat model of MCAO was established via bilateral common carotid artery ligation. Three groups of rats with the same baseline were selected (model group, positive drug group, and control group) according to their behavioral score and body weight 24 h after surgery. Drug administration was initiated on post-MCAO day 8 and was continued for 28 days. Mobile phones were used to collect facial images at different time points after surgery. In facial image analysis, the outer canthi of both eyes were used as the facial dividing line, and the outer edge of the rat's face was framed using the marking method, and the framed part was regarded as the facial area (S) of the rats. The histogram created with Photoshop CS5 was used to measure the face area in pixels. The distance between the outer canthi of both eyes (Le) and vertical line from the tip of the nose to the line joining the eyes was recorded as H1, and the line from the tip of the nose to the midpoint of the line joining the eyes was recorded as H2. The facial area was calibrated based on the relationship between H1 and H2. The distance between the eyes was inversely proportional to the distance between the rats and mobile phone such that the face area was calibrated by unifying Le. The size of Le between the eyes was inversely proportional to the distance between the rats and mobile phone. This was used to calibrate the face area. When compared with the control group, the facial area of the model group gradually increased from postoperative day 1 to day 7, and there was a significant difference in the facial area of the model group on postoperative day 7. Hence, positive drugs exhibited the effect of improving facial swelling. H1 and H2 can reflect the state of turning the head and raising the head of the rats, respectively. Facial area was calibrated according to the relationship between H1 and H2, which had no obvious effect on the overall conclusion. Furthermore, mobile phone lens was used to capture the picture of rat face, and the distance between the eyes and H1 and H2 was used to calibrate the facial area. Hence, this method is convenient and can be used to evaluate subjective judgment of the human eyes via a quantitative method.

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Experimental rat model of chronic cerebral hypoperfusion–reperfusion mimicking normal perfusion pressure breakthrough phenomenon

Cited by 2 publications

References 17 publications

Neuroprotective effect of indomethacin in normal perfusion pressure breakthrough phenomenon

Neuroprotective effect of indomethacin in normal perfusion pressure breakthrough phenomenon

Development of a Method for Quantitative Evaluation of Facial Swelling in a Rat Model of Cerebral Ischemia by Facial Image Processing

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