Experimental rapid infection in market swine following exposure to a Salmonella contaminated environment

Hs, Hurd; Jk, Gailey; Jd, McKean; Mh, Rostagno

doi:10.31274/safepork-180809-238

Cited by 23 publications

(21 citation statements)

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“…According to previous studies, Salmonella was able to invade the tonsils within 30 min after oral uptake or contact with the contamination source and within a few hours (2 to 3 h p.i.) colonized the mandibular lymph nodes, colon, cecum, and ileocaecal lymph nodes (5,14,21). Results of our investigations show that both the qualitative and quantitative manifestations of Salmonella in the tonsils and colon were significantly greater for the probiotic group than for the control group, but only the tonsil samples showed a continued high Salmonella concentration at the end of this study (Fig.…”

Section: Discussionmentioning

confidence: 49%

Influence of a Probiotic Strain ofEnterococcus faeciumonSalmonella entericaSerovar Typhimurium DT104 Infection in a Porcine Animal Infection Model

Szabó

Wieler

Tedin

et al. 2009

Appl Environ Microbiol

103

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The beneficial effects of probiotic Enterococcus spp. in different hosts, such as mice and humans, have previously been reported in several studies. However, studies of large domestic animals, as well as challenge studies with pathogenic microorganisms, are very rare. Here, we investigated the influence of oral treatment of pigs with the probiotic bacterium Enterococcus faecium NCIMB 10415 on Salmonella enterica serovar Typhimurium DT104 infections in weaning piglets. Clinical symptoms, fecal excretion, the organ distribution of Salmonella, and the humoral immune response (immunoglobulin G [IgG], IgM, and IgA levels) in serum were examined. A pool of 89 piglets was randomly divided into probiotic and control groups. The probiotic group received a feed supplement containing E. faecium starting on day 14 postpartum prior to challenge with Salmonella serovar Typhimurium DT104 at 28 days postpartum. After challenge with Salmonella serovar Typhimurium DT104, piglets in both groups showed no severe clinical signs of salmonellosis. However, fecal excretion and colonization of Salmonella in organs were significantly greater in piglets fed E. faecium. Likewise, the humoral immune response against Salmonella (serum IgM and IgA levels) was significantly greater in the probiotic group animals than in control animals. The results of this study suggest that E. faecium NCIMB 10415 treatment enhanced the course of infection in weaning piglets challenged with Salmonella serovar Typhimurium DT104. However, the probiotic treatment also appeared to result in greater production of specific antibodies against Salmonella serovar Typhimurium DT104.

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Section: Discussionmentioning

confidence: 49%

Influence of a Probiotic Strain ofEnterococcus faeciumonSalmonella entericaSerovar Typhimurium DT104 Infection in a Porcine Animal Infection Model

Szabó

Wieler

Tedin

et al. 2009

Appl Environ Microbiol

103

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“…I serotypes in fecal samples or the intestine increases further, with 10 to 15% of animals testing positive (13,44). A body of evidence shows that the main factor responsible for spreading the infection among animals on the farm or among animals during transport to slaughter is fecal contamination of the environment (14,16,25,32,45,46). These investigations suggest that fecal shedding of S. enterica subsp.…”

Section: Discussionmentioning

confidence: 88%

Molecular and Phenotypic Analysis of the CS54 Island ofSalmonella entericaSerotype Typhimurium: Identification of Intestinal Colonization and Persistence Determinants

et al. 2003

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The shdA gene is carried on a 25-kb genetic island at centisome 54 (CS54 island) of the Salmonella enterica serotype Typhimurium chromosome. In addition to shdA, the CS54 island of Salmonella serotype Typhimurium strain LT2 contains four open reading frames designated ratA, ratB, sivI, and sivH. DNA hybridization analysis revealed that the CS54 island is comprised of two regions with distinct phylogenetic distribution within the genus Salmonella. Homologues of shdA and ratB were detected only in serotypes of Salmonella enterica subsp. I. In contrast, sequences hybridizing with ratA, sivI, and sivH were present in S. enterica subsp. II and S. bongori in addition to S. enterica subsp. I. Deletion of the ratA and sivI genes did not alter the ability of Salmonella serotype Typhimurium to colonize the organs of mice. Insertional inactivation of the sivH gene resulted in defective colonization of the Peyer's patches of the terminal ileum but normal colonization of the cecum, mesenteric lymph nodes, and spleen. Deletion of the shdA gene resulted in decreased colonization of the cecum and Peyer's patches of the terminal ileum and colonization to a lesser degree in the mesenteric lymph nodes and spleen 5 days post-oral inoculation of mice. A strain containing a deletion in the ratB gene exhibited a defect for the colonization of the cecum but not of the Peyer's patches, mesenteric lymph nodes, and spleen. The shdA and ratB deletion strains exhibited a shedding defect in mice, whereas the sivH deletion strain was shed at numbers similar to the wild type. These data suggest that colonization of the murine cecum is required for efficient fecal shedding in mice.

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“…The importance of stringent implementation of the all-in/all-out hygiene procedure in the farrowing section including cleaning and disinfection was reported as a protective factor for Salmonella seroconversion in European market-age pigs by Lo Fo Wong et al in 2004 [18]. The presence of residual Salmonella was found to be a source of contamination for incoming pigs in an experimental study [11] and in an observational survey [3]. In the latter study, residual environmental Salmonella contamination of the fattening room before placing the batch was found to increase the risk of Salmonella shedding of the finishing batch [3].…”

Section: Discussionmentioning

confidence: 91%

Risk factors forSalmonellaseroconversion of fattening pigs in farrow-to-finish herds

et al. 2007

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-We did a prospective observational 9-month long study to quantify risk factors of managerial and hygiene practices, and pig-health status for Salmonella seroconversion of fattening pigs reared in subclinically infected French farrow-to-finish farms. During the fattening phase, 2 649 pigs belonging to the same batch of contemporary pigs, from 89 conventional farrow-to-finish farms were individually followed and regularly blood sampled on a monthly basis. Farm recruitment was based on the farmer's willingness to cooperate. Pig status was assessed using an indirect ELISA test. Evolution of the serological status was studied by means of survival analysis. A Cox proportionalhazards model, taking into account the clustering of animals at the farm level, was used to examine the effects of explanatory variables on the time to Salmonella seroconversion of pigs. Applying group level antibiotic treatment to the pigs during the fattening period (Hazard Ratio (HR) = 2.4; 95% CI: 1.7, 3.4) was identified as a risk factor for Salmonella seroconversion, as the presence of residual Salmonella contamination in the fattening pen before placing the pigs into the pens (HR = 1.9; 95% CI: 1.2, 2.9). Porcine reproductive and respiratory syndrome virus (PRRSV) seropositivity during the fattening period also indicated an increased hazard for seroconversion (HR = 1.6; 95% CI: 1.1, 2.5). The batch size was identified as a risk factor for Salmonella seroconversion: the higher the number of pigs was in the fattening room followed, the higher was the risk (HR +10pigs = 1.05 for a 10-pig increment; 95% CI: 1.03, 1.06). The biosecurity measures of wearing specific clothes before entering the facilities (HR = 0.5; 95% CI: 0.3, 0.9) and enclosing the pig farm facilities were protective (HR = 0.4; 95% CI: 0.2, 0.8).Salmonella / pigs / seroconversion / risk factors / survival analysis

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Experimental rapid infection in market swine following exposure to a Salmonella contaminated environment

Cited by 23 publications

References 0 publications

Influence of a Probiotic Strain ofEnterococcus faeciumonSalmonella entericaSerovar Typhimurium DT104 Infection in a Porcine Animal Infection Model

Influence of a Probiotic Strain ofEnterococcus faeciumonSalmonella entericaSerovar Typhimurium DT104 Infection in a Porcine Animal Infection Model

Molecular and Phenotypic Analysis of the CS54 Island ofSalmonella entericaSerotype Typhimurium: Identification of Intestinal Colonization and Persistence Determinants

Risk factors forSalmonellaseroconversion of fattening pigs in farrow-to-finish herds

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