Experimental production of siderocytes.

Deiss, Andrew; Kurth, Doris; Cartwright, G. E.; Wintrobe, M. M.

doi:10.1172/jci105350

Cited by 19 publications

(7 citation statements)

References 20 publications

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“…It has been found, in confirmation of earlier studies (1), that when reticulated-siderocytes (R-S cells) were incubated in vitro the granules disappeared from the cells. The rate of loss of the granules exceeded the rate of maturation of the reticulum with the result that R-S cells were converted to granule-free reticulocytes (R cells).…”

Section: Discussionsupporting

confidence: 85%

“…Had the reticulum disappeared more rapidly than the granules, S cells would have resulted. Thus, both in vivo (1) and in vitro, the sequence of maturation was from R-S cells to R cells to mature erythrocytes.…”

Section: Discussionmentioning

confidence: 99%

“…The details of the diet and dietary supplements have been described previously (1). Beginning at approximately 5-6 wk of age, daily phlebotomy by jugular puncture was begun.…”

Section: Methodsmentioning

confidence: 99%

“…It has been recognized from experimental studies in swine (1,2) that the physiologic significance and metabolic behavior of siderotic granules contained within nonreticulated, mature erythrocytes (S cells) is quite different from such granules contained within reticulocytes (reticulated-siderocytes, R-S cells).…”

Section: Introductionmentioning

confidence: 99%

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Ferritin metabolism in reticulated-siderocytes

Deiss¹,

Cartwright²

1970

J. Clin. Invest.

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A B S T R A C T Reticulated-siderocytes (reticulocytes which contain siderotic granules), obtained from the circulation of pigs after vigorous phlebotomy, were incubated in vitro. A rapid disappearance of granules from the reticulocytes was observed over 24 hr. Simultaneously with the decrease in granules, soluble ferritin accumulated in the media and siderotic granules developed in monocytes. The disappearance of the granules from the reticulated-siderocytes was oxygen-dependent and the loss of granules and the accumulation of ferritin in the media were both prevented by the addition of cyanide or dinitrophenol.It is concluded that the ferritin aggregates in the granules of reticulated-siderocytes are dispersed intracellularly into soluble ferritin, that soluble ferritin is excreted from the cell, and that one or both of these steps is dependent upon oxidative metabolism. Blood monocytes are capable of taking up soluble ferritin from the media and converting this into siderotic granules. Thus, a reticulocyte to plasma to monocyte ferritin pathway has been described.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

“…The details of the diet and dietary supplements have been described previously (1). Beginning at approximately 5-6 wk of age, daily phlebotomy by jugular puncture was begun.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Ferritin metabolism in reticulated-siderocytes

Deiss¹,

Cartwright²

1970

J. Clin. Invest.

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“…Given what is known about the pathogenesis of SA and the predicted structural features of Sfxn1, it is likely that the protein facilitates the transport of a component required for iron utilization into or out of the mitochondria. An abundance of iron-laden mitochondria in mature anucleate erythrocytes (siderocytes) is distinctive among the SAs, and, outside of the f/f mutant, has been described only in severely pyridoxine-deficient juvenile swine or neonatal rodents (Deiss et al 1966;Hammond et al 1969;Keyhani et al 1974). This suggests that pyridoxine, or a derivative thereof, may be transported by Sfxn1 into the mitochondria.…”

Section: Discussionmentioning

confidence: 99%

A mutation in a mitochondrial transmembrane protein is responsible for the pleiotropic hematological and skeletal phenotype of flexed-tail (f/f) mice

Fleming¹,

Campagna²,

Haslett³

et al. 2001

Genes Dev.

120

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We have studied the flexed-tail (f) mouse to gain insight into mammalian mitochondrial iron metabolism. Flexed-tail animals have axial skeletal abnormalities and a transient embryonic and neonatal anemia characterized by pathologic intramitochondrial iron deposits in erythrocytes. Mitochondrial iron accumulation is the hallmark of sideroblastic anemias, which typically result from defects in heme biosynthesis or other pathways that lead to abnormal erythroid mitochondrial iron utilization. To clone the f gene, we used positional cloning techniques, and identified a frameshift mutation in a mitochondrial transmembrane protein. The mutated gene, Sfxn1, is the prototype of a novel family of evolutionarily conserved proteins present in eukaryotes.

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Haem Synthesis in Sideroblastic Anaemia

Konopka

1977

Novartis Foundation Symposia

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Experimental production of siderocytes.

Cited by 19 publications

References 20 publications

Ferritin metabolism in reticulated-siderocytes

Ferritin metabolism in reticulated-siderocytes

A mutation in a mitochondrial transmembrane protein is responsible for the pleiotropic hematological and skeletal phenotype of flexed-tail (f/f) mice

Haem Synthesis in Sideroblastic Anaemia

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