Experimental preretinal neovascularization by laser-induced venous thrombosis in rats

Abstract

This is a new model of retinal ischemia and associated neovascularization established by venous thrombosis that is easily reproducible. Many aspects of rat retinal physiology are known and this model has promise as an avenue for further investigation.

Quantitative grading of preretinal neovascularization in adult rats

“…These results indicate that FITC-dextran angiography is a good method of quantifying preretinal neovascularization of the entire retina. Saito et al (1997) reported a 70% (19/27) rate of preretinal neovascularization production in pigmented adult rats using photodynamic venous occlusion with systemic fluorescein dye injections examined by FAG. Kang et al (1999) reported a 90% (9/10) rate of preretinal neovascularization production in albino adult rats using photodynamic venous occlusion with systemic rose bengal dye injections examined by ADPase staining.…”

“…According to previous studies, preretinal neovascularization is definitely present 14 days after venous occlusion (Saito et al 1997;Kang et al 1999). Therefore, animals were killed accordingly in this experiment.…”

“…Preretinal neovascularization was created according to the methodology of previous authors with minor modifications (Ham et al 1997;Saito et al 1997;Kang et al 1999;Park et al 2002). Rose bengal sodium salt (certified purity 90%; Sigma, St Louis, Missouri, USA) was diluted with phosphate-buffered saline (PBS) to 50 mg/ ml, and sterile-filtered through a 0.45 mm filtering unit.…”

“…Fluorescein angiography (FAG), adenosine diphosphatase (ADPase) staining and histological study have been utilized to assess preretinal neovascularization induced by photodynamic venous occlusion in adult rats (Danis 1993;Ham et al 1997;Saito et al 1997;Kang et al 1999;Park et al 2002). However, because fluorescein molecules are small, they can leak out of vessels and yield different sizes of images at different times in preretinal neovascularization (Ham et al 1997).…”

Recombinant Adeno-Associated Virus Vector Expressing Angiostatin Inhibits Preretinal Neovascularization in Adult Rats

“…Twenty-one days after viral injections, PNV was induced by laser-induced retinal vein occlusion according to an established method with modifications [23,24]. Rose bengal sodium salt (certified purity 90%; Sigma, St. Louis, Mo., USA) was prepared at a concentration of 50 mg/ml with an isotonic sodium chloride solution.…”