Experimental pharmacological research regarding the antidepressant effect of associating doxepin and selegiline in normal mice

Abstract

The severity and complexity of depression can vary widely among individuals, thus making single drug therapy ineffective in some cases. Taking this fact into account and using a mouse model, we set on investigating the possibility of obtaining a synergism of action between a classical tricyclic antidepressant that inhibits noradrenalin and serotonin reuptake (doxepin), and a modern antidepressant that inhibits type-B monoamine oxidase (selegiline). We measured the antidepressant effect using the forced swimming test and the tail suspension test. We determined motor activity using the Activity Cage test. Our results have shown that the antidepressant effect intensifies significantly in the animals treated with both antidepressants simultaneously compared to those treated only with doxepin. Furthermore, we observed that selegiline decreases the sedative effect of doxepin in the Activity Cage test.

Doxepin Exacerbates Renal Damage, Glucose Intolerance, Nonalcoholic Fatty Liver Disease, and Urinary Chromium Loss in Obese Mice

“…We then separated the mice into two groups: one treated with 5 mg/kg doxepin (Sigma-Aldrich, St Louis, MO, USA) and another treated with saline (vehicle control) via daily gavage for the final 56 days of the HFD-feeding duration (39.38 ± 0.96 g for treated mice vs. 39.48 ± 1.23 g for control mice, p > 0.05). Here we based the doxepin dosage on mouse studies on how doxepin affects glucose homeostasis, behavior, depression, stress, and memory deficits [ 29 , 81 , 82 ]. In our preliminary investigation, obese mice were orally administered 2 mg/kg doxepin; however, their body weight and body weight gain at this dosage did not differ significantly from those of the obese control mice ( Figure S10 ).…”

Effects of Venlafaxine, Risperidone and Febuxostat on Cuprizone-Induced Demyelination, Behavioral Deficits and Oxidative Stress

“…The locomotor activity was assessed using a system composed of a cage with transparent plexiglass walls (40 × 40 × 25 cm), equipped with two sets of photo beams (Ugo Basile, Gemonio, VA, Italy). Mice were placed individually in the apparatus and the total photo-beam interruptions were measured for 5 min, corresponding to total horizontal and vertical movements performed by each animal [65,66].…”