Experimental pathophysiology of systemic alterations induced by Bothrops asper snake venom

Gutiérrez, José Marı́a; Escalante, Teresa; Rucavado, Alexandra

doi:10.1016/j.toxicon.2009.01.039

Cited by 140 publications

(110 citation statements)

References 79 publications

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“…These local manifestations may lead to permanent sequelae, such as tissue loss and dysfunction (Dart et al, 1992;Otero et al, 2002). After systemic venom distribution, and depending on the severity of the case, viperid snakebite envenomings are characterized by coagulopathies, bleeding, renal alterations and hemodynamic manifestations which may lead to cardiovascular shock and multisystem organ failure (Gutiérrez et al, 2009b;Warrell, 2004). Intravascular hemolysis might also occur, in some cases associated with microthrombi formation (Warrell, 1996).…”

Section: Clinical Manifestations Of Envenomingmentioning

confidence: 99%

Snakebite Envenoming: A Public Health Perspective

Gutiérrez¹

2012

Public Health - Methodology, Environmental and Systems Issues

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Section: Clinical Manifestations Of Envenomingmentioning

confidence: 99%

Snakebite Envenoming: A Public Health Perspective

Gutiérrez¹

2012

Public Health - Methodology, Environmental and Systems Issues

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“…15 A recent review of the systemic alterations induced by the venom of Bothrops asper suggested that the complex toxicity of venom components goes beyond their effects on the coagulation cascade and the number and function of platelets, at least in animal models. 16 Other toxic effects of the venom include an increase in vascular permeability, an induction of abnormal release of tumor necrosis factor and cytokines, and most importantly, a systemic endothelial dysfunction. The latter may be the pathophysiological substrate to explain the development of posterior reversible encephalopathy in our patient, because he never developed systemic hypertension or hypovolemic shock, which would explain a primary loss of autoregulation of cerebral blood vessels.…”

Section: Commentmentioning

confidence: 99%

Reversible Posterior Leukoencephalopathy in a Venomous Snake (Bothrops asper) Bite Victim

Delgado¹,

Brutto²

2012

The American Journal of Tropical Medicine and Hygiene

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Abstract. An 18-year-old man developed posterior reversible leukoencephalopaty after being bitten by a venomous snake (Bothrops asper). It is possible that this previously unrecognized neurological complication of snake bite envenoming occurred as the result of endothelial dysfunction induced by the venom of the offending snake. This pathogenetic mechanism has also been implicated as the cause of cerebral infarctions in snake bite victims. Alternatively, the leukoencephalopathy might have been a complication of antivenom therapy.

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“…Experimental studies made with Bothrops venom suggest a multifactorial pathogenesis for ARF which includes a variety of mechanisms [2]. In order to elucidate the mechanism of direct nephrotoxicity the aim of the present work was to investigate the effect of B. pauloensis venom (BpV) on renal epithelial cells.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity of Bothrops pauloensis Venom on Madin-Darby Canine Kidney Cells: Cell Death Mechanism

Ad¹,

Ico²,

Db³

et al. 2017

J Kidney

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Bothrops is a genus of pit vipers (snakes) endemic to Central and South America. Snake envenomation (Bothrops genus) specifically Bothropoides pauloensis, a venomous snake popularly known as "Jararaca pintada", which is found in the Brazilian territory or more commonly in the southwest of the state of Sao Paulois. Acute kidney injury is one of the complications observed in Bothrops snakebite with relevant morbidity and mortality. Here, we showed the cytotoxic effect of Bothropoides pauloensis venom on MDCK cells, intracellular mitochondrial membrane potentiality (ΔΨm), BpV regulated Reactive Oxygen Species (ROS) and activation of caspases 3 and 7 in MDCK cells. MDCK cells were treated with different concentrations of B. pauloensis venom for 24 hrs and cell death were measured with annexin V and PI staining and detected by flow cytometry. MDCK cells were treated with two different concentrations (IC50 and 2 × IC50) of BpV after 24 hrs. All data are expressed as mean ± SEM of three independent experiments with six replicates (ANOVA and Dunnett test, *p<0.05). Cytotoxicity was assessed by MTT assay and the treatment with BpV caused decrease in cell viability, with an IC50 of 7.5 µg/mL. After the treatment, amounts of ROS in the BpV-treated MDCK-cells (7.5 µg/mL) showed significant right dislocation of the fluorescence peak in the histogram, when compared with the untreated control group. Caspase 3 and 7 activity was determined in presence of the fluorogenic Ac-DEVDafc substrate after treatment with Bothropoides pauloensis venom (12 hrs) then the result indicated apoptotic involvement in BpV-induced cell death. Similar results were also found for venoms of other Bothrops and Bothropoides genus snakes such as B. alternatus, B. insularis and B. leucurus.

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Experimental pathophysiology of systemic alterations induced by Bothrops asper snake venom

Cited by 140 publications

References 79 publications

Snakebite Envenoming: A Public Health Perspective

Snakebite Envenoming: A Public Health Perspective

Reversible Posterior Leukoencephalopathy in a Venomous Snake (Bothrops asper) Bite Victim

Cytotoxicity of Bothrops pauloensis Venom on Madin-Darby Canine Kidney Cells: Cell Death Mechanism

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