Experimental Otitis Media with Effusion Induced by Lipopolysaccharide from Klebsiella pneumoniae<i>Mucociliary Pathology of the Eustachian Tube</i>

Ōhashi, Y.; Nakai, Yousuke; Esaki, Yusuke; Ohno, Yoshiharu; Sugiura, Yoshikazu; Okamoto, Hideki

doi:10.3109/00016489109134989

Cited by 17 publications

(18 citation statements)

References 18 publications

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“…Dai and Gan (2008) studied TM properties in guinea pigs with MEEs caused by an injection of lipopolysaccharide (LPS) into the middle ear. Injection of LPS into the middle-ear space causes inflammation and subsequent effusion (Ohashi et al 1991). All LPSinjected ears had a middle-ear space that was at least half-filled with fluid.…”

Section: Discussionmentioning

confidence: 99%

Conductive Hearing Loss Induced by Experimental Middle-Ear Effusion in a Chinchilla Model Reveals Impaired Tympanic Membrane-Coupled Ossicular Chain Movement

Thornton

Chevallier²,

Koka³

et al. 2013

JARO

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Otitis media with effusion (OME) occurs when fluid collects in the middle-ear space behind the tympanic membrane (TM). As a result of this effusion, sounds can become attenuated by as much as 30-40 dB, causing a conductive hearing loss (CHL). However, the exact mechanical cause of the hearing loss remains unclear. Possible causes can include altered compliance of the TM, inefficient movement of the ossicular chain, decreased compliance of the oval window-stapes footplate complex, or altered input to the oval and round window due to conduction of sound energy through middle-ear fluid. Here, we studied the contribution of TM motion and umbo velocity to a CHL caused by middle-ear effusion. Using the chinchilla as an animal model, umbo velocity (V U ) and cochlear microphonic (CM) responses were measured simultaneously using sinusoidal tone pip stimuli (125 Hz-12 kHz) before and after filling the middle ear with different volumes (0.5-2.0 mL) of silicone oil (viscosity, 3.5 Poise). Concurrent increases in CM thresholds and decreases in umbo velocity were noted after the middle ear was filled with 1.0 mL or more of fluid. Across animals, completely filling the middle ear with fluid caused 20-40-dB increases in CM thresholds and 15-35-dB attenuations in umbo velocity. Clinic-standard 226-Hz tympanometry was insensitive to fluid-associated changes in CM thresholds until virtually the entire middle-ear cavity had been filled (approximately 91.5 mL). The changes in umbo velocity, CM thresholds, and tympanometry due to experimentally induced OME suggest CHL arises primarily as a result of impaired TM mobility and TM-coupled umbo motion plus additional mechanisms within the middle ear.

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Section: Discussionmentioning

confidence: 99%

Conductive Hearing Loss Induced by Experimental Middle-Ear Effusion in a Chinchilla Model Reveals Impaired Tympanic Membrane-Coupled Ossicular Chain Movement

Thornton

Chevallier²,

Koka³

et al. 2013

JARO

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“…Each group contained 6 guinea pigs. These times reflect early and chronic stages, respectively, of OME after LPS injection [Ohashi et al, 1991].…”

Section: Experimental Protocolmentioning

confidence: 99%

“…In this study, we induced OME in guinea pigs via lipopolysaccharide (LPS) injection into the middle ear [Ohashi et al, 1991] and measured subsequent changes in the movement of the BM and tympanic membrane (TM) with a laser Doppler vibrometer. The changes observed in BM mobility at the basal turn and apex in OME ears suggest that OME may directly affect cochlear function through changing the ratio of stapes to umbo velocity over a frequency range of 200 Hz to 40 kHz.…”

Section: Introductionmentioning

confidence: 99%

Change in Cochlear Response in an Animal Model of Otitis Media with Effusion

Dai

Gan²

2009

Audiol Neurotol

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Our previous studies confirm that middle ear mobility is reduced in the presence of otitis media with effusion (OME). Variations in middle ear function may result in changes in cochlear response in OME ears. With the long-term goal of evaluating cochlear function in OME ears, the aim of this study was to measure the displacement of the basilar membrane (BM) in guinea pig ears with OME. Vibrations of the BM at the apex and basal turn were measured in an in vitro preparation extracted 3 and 14 days after injection of lipopolysaccharide in the middle ear of guinea pigs. The results show that the displacement sensitivity of the BM at the apex and the basal turn to sound pressure in the ear canal was reduced up to 25 dB at their characteristic frequencies, respectively. Cochlear gain with respect to umbo movement was also changed in ears with OME in both groups. This study provides data for analysis of the change of BM vibration in a guinea pig OME model.

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“…The peptide solutions were diluted 1:100 with acetonitrile/ water/TFA 50/50/0.2 (v/v/v) and 1 ml of each solution was mixed with 1 ml of a 10 mg/ml solution of a-cyano-4-hydroxycinnamic acid (4-ACH) in acetonitrile/water/TFA 50/50/0.2 (v/v/v) containing a mass reference peptide (VNTPEHVV-PYGLGSPSRS, bovine big-endothelin (22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39), MH av + = 1897.10).…”

Section: Maldi-tof Mass Spectrometry Analysismentioning

confidence: 99%

“…In a large part of these infections bacterial products, like lipopolysaccharide (LPS) from Gram-negative bacteria and lipoteichoic acid (LTA) from Gram-positive bacteria, are involved [15,28]. These products can induce an inflammatory reaction in the middle ear or in the sinuses and can cause injury to the mucosa of the upper airway epithelia [25,26].…”

Section: Introductionmentioning

confidence: 99%

Development of novel LL-37 derived antimicrobial peptides with LPS and LTA neutralizing and antimicrobial activities for therapeutic application

et al. 2006

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Experimental Otitis Media with Effusion Induced by Lipopolysaccharide from Klebsiella pneumoniaeMucociliary Pathology of the Eustachian Tube

Cited by 17 publications

References 18 publications

Conductive Hearing Loss Induced by Experimental Middle-Ear Effusion in a Chinchilla Model Reveals Impaired Tympanic Membrane-Coupled Ossicular Chain Movement

Conductive Hearing Loss Induced by Experimental Middle-Ear Effusion in a Chinchilla Model Reveals Impaired Tympanic Membrane-Coupled Ossicular Chain Movement

Change in Cochlear Response in an Animal Model of Otitis Media with Effusion

Development of novel LL-37 derived antimicrobial peptides with LPS and LTA neutralizing and antimicrobial activities for therapeutic application

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