Experimental osteoarthritis induced by selective myectomy and tendotomy

Arsever, Christiane L.; Bole, Giles G.

doi:10.1002/art.1780290214

Cited by 33 publications

(23 citation statements)

References 23 publications

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“…However, Arsever and Bole (7) noted bilateral lesions in the knee joints of Hartley guinea pigs that had undergone unilateral myectomy and tendotomy; the lesions they described resemble the spontaneous lesions in the knee joints of the guinea pigs we studied. It may be that surgical manipulation and alteration of weight bearing enhanced the tendency for spontaneous cartilage degeneration to occur in their animals, but there remains the question of why some spontaneous lesions were not observed in the sham operated or normal control animals of similar ages and weights (7). The animals they used would have been approximately 8 months old and would have weighed more than 1,000 gm, weights and ages at which fairly striking spontaneous changes in the articular cartilage were seen in the guinea pigs we studied.…”

Section: Discussionmentioning

confidence: 99%

Spontaneous cartilage degeneration in guinea pigs

Bendele

Hulman

1988

Arthritis & Rheumatism

190

109

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Spontaneous degeneration of the knee joint cartilage in male Hartley guinea pigs was studied by light microscopy in animals aged S 1 8 months. Unilateral focal degeneration, characterized by chondrocyte death and proteoglycan loss with surface fibrillation, was observed on the medial tibial plateau in 2 of 5 guinea pigs that were 3 months old. The incidence and severity of the lesions increased with age, and by 12-18 months of age, all animals had moderate-to-severe degeneration of the medial tibial plateau, femoral condyle, and meniscus. Lesions were not present on the lateral aspect of the knee joint in any of the animals.Naturally occurring and experimentally induced cartilage degeneration has been described in a variety of laboratory and domestic animal species (1-3). Recently, several different models of experimentally induced osteoarthritis have been described in mature guinea pigs, but in none of these has mention been made of any significant incidence of spontaneous cartilage degeneration (4-9).Over the course of experimentation with various surgical and nonsurgical methods of inducing cartilage degeneration in mature male and female Hartley guinea pigs, we observed a high incidence of spontaneous cartilage degeneration of the medial tibial plateau, meniscus, and femoral condyle. This occurred not only in the contralateral joints of animals

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Section: Discussionmentioning

confidence: 99%

Spontaneous cartilage degeneration in guinea pigs

Bendele

Hulman

1988

Arthritis & Rheumatism

190

109

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“…The control knees of the rabbits destabilised for 12 weeks showed gross and biochemical evidence of mild to moderate DJD. The reasons for this are unclear, but similar results were also obtained in the control knee of guinea pigs who underwent selective myectomy and tenotomy [8], and similar cartilage abnormalities do develop in the joint of the contralateral unoperated hind limb. The pattern of use of the different limbs was not specifically monitored, but it is possible that joint instability is more invalidating than immobilisation.…”

Section: Discussionmentioning

confidence: 59%

“…Many experimental methods have been used to induce DJD in animals [4][5][6][7][8]10,12,13]. Only some of these experimental models reflect the course of the condition in humans [22], with relatively slow initial progression.…”

Section: Discussionmentioning

confidence: 99%

“…Most of the information on biochemical and pathological changes in early DJD has been obtained from animal models [3]. These have included sectioning of the anterior cruciate ligament (ACL), in isolation [4,5] or coupled with medial meniscectomy [6], sectioning of one collateral ligament and meniscectomy [7], and myotomy and tenotomy [8]. Other methods such as the use of animals who naturally develop DJD [9], immobilisation [10][11][12], and intra-articular injection of proteases [13] have also been used.…”

Section: Introductionmentioning

confidence: 99%

“…As the cost of maintaining primates and large mammals for research purposes is very high, it is costeffective to use readily available small animals able to produce sufficient amounts of tissue [8]. We therefore set out to investigate the histological and biochemical changes in experimental DJD of the rabbit knee using either the immobilisation or the destabilisation methods in two separate groups of animals, with the untreated contralateral joint used as the control.…”

Section: Introductionmentioning

confidence: 99%

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Articular cartilage lesions of the knee following immobilisation or destabilisation for 6 or 12 weeks in rabbits

Maffulli

Yip

et al. 1998

Clin Rheumatol

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Eighty mature female New Zealand White rabbits were sacrificed 6 or 12 weeks after either section of the medial collateral and the anterior cruciate ligaments with removal of the anterior third of the medial meniscus of one knee, or immobilisation of one knee, using the contralateral non-treated knee as the control. The area of degenerated joint surface of the distal femur, and water and proteoglycan content were measured, and the articular cartilage stained using haematoxylin and eosin and safranin O. Destabilisation resulted in a significantly larger time-dependent degenerated joint surface area. Water content significantly increased after 6 weeks with no significant differences between immobilisation and destabilisation. Destabilisation resulted in a significantly greater decrease in proteoglycan content. At 12 weeks, the control knees of the animals undergoing destabilisation showed significant degenerative changes. There were more extensive lesions in destabilisation, while 6 weeks of immobilisation produced moderate degenerative joint disease.

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Effects of salicylate on chondrocytes from osteoarthritic and contralateral knees of dogs with unilateral anterior cruciate ligament transection

Slowman‐Kovacs

Albrecht

Brandt

1989

Arthritis & Rheumatism

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Salicylates suppress net glycosaminoglycan synthesis in articular cartilage. The inhibitory effect is greater in osteoarthritic (OA) cartilage than in normal cartilage. Whether the isolated OA chondrocyte is inherently more susceptible to the effects of salicylate on glycosaminoglycan metabolism has not been determined. The results of this study show that, after isolation from the extracellular matrix, normal and OA chondrocytes in suspension culture are similarly susceptible to the metabolic effects of salicylate. However, chondrocytes from the contralateral knees of dogs with unilateral OA were notably resistant to the effects of salicylate.Salicylates produce reversible, concentrationdependent suppression of proteoglycan (PG) synthesis by normal articular cartilage in vitro. The effect is much greater in osteoarthritic (OA) cartilage than in normal cartilage (1). Since uptake of ''C-acetylsalicylic acid by slices of OA cartilage has been shown to be significantly greater than that by normal cartilage (I), the depletion of negatively charged PGs in the matrix of OA cartilage presumably facilitated diffusion of the weakly acidic salicylate into the matrix. This possibility was supported by data demonstrating increased suppression of PG synthesis in normal cartilage after enzymatic depletion of its PGs (2).The above data do not exclude the additional possibility that in OA, the chondrocyte, which is intrinsically hypermetabolic (3,4), may be more susceptible than normal to the effects of salicylate. In the present study, we used isolated chondrocytes in suspension cultures, rather than cartilage organ cultures, to examine the susceptibility of the OA chondrocyte to salicylate. MATERIALS AND METHODSSurgical procedures. OA was induced in a standard manner in 9 normal adult mongrel dogs (25-30 kg) by transection of the anterior cruciate ligament of the right knee (1). Postoperatively, all dogs bore weight on the operated limb by the third day and ambulated freely in caged runs (4 feet x 8 feet) until they were killed 10-13 weeks later. Nine additional adult mongrel dogs that underwent no surgical procedure were used as normal controls.Tissues. At the time the dogs were killed, fullthickness samples of the articular cartilage and underlying subchondral bone were obtained from the central regions of the habitually loaded area of the medial femoral condyle of both knees of each animal for histologic study. Slices of articular cartilage (10-15 mg wet weight) were taken from the same sites for determination of dry weight and uronic acid concentration. The remainder of the cartilage from the articular surfaces of the medial and lateral femoral condyles and tibia1 plateaus was used for isolation of chondrocytes.

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Experimental osteoarthritis induced by selective myectomy and tendotomy

Cited by 33 publications

References 23 publications

Spontaneous cartilage degeneration in guinea pigs

Spontaneous cartilage degeneration in guinea pigs

Articular cartilage lesions of the knee following immobilisation or destabilisation for 6 or 12 weeks in rabbits

Effects of salicylate on chondrocytes from osteoarthritic and contralateral knees of dogs with unilateral anterior cruciate ligament transection

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