Experimental Mycobacterium microti Infection in Bank Voles (Myodes glareolus)

Vidal, Enríc; Burgaya, Judit; Michelet, Lorraine; Arrieta-Villegas, Claudia; Cantero, Guillermo; Cruz, Krystel de; Tambosco, Jennifer; Bari, Michelle Di; Nonno, Romolo; Boschiroli, Maria‐Laura; Val, Bernat Pérez de

doi:10.3390/microorganisms10010135

Cited by 4 publications

(2 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Amplification was performed in a 7500 fast real-time PCR system (Applied Biosystems, Walham, MA, USA). CFU genomic equivalents were calculated as previously described 44 .…”

Section: Multi-cytokine Quantification Assaymentioning

confidence: 99%

A proof-of-concept study to investigate the efficacy of heat-inactivated autovaccines in Mycobacterium caprae experimentally challenged goats

Melgarejo

Planas

Cobos

et al. 2022

Sci Rep

Self Cite

View full text Add to dashboard Cite

This study aimed to assess the efficacy of a heat-inactivated Mycobacterium caprae (HIMC) vaccine in goats experimentally challenged with the same strain of M. caprae. Twenty-one goats were divided into three groups of seven: vaccinated with heat-inactivated Mycobacterium bovis (HIMB), with HIMC and unvaccinated. At 7 weeks post-vaccination all animals were endobronchially challenged with M. caprae. Blood samples were collected for immunological assays and clinical signs were recorded throughout the experiment. All goats were euthanized at 9 weeks post-challenge. Gross pathological examination, analysis of lung pathology using computed tomography, and bacterial load quantification in pulmonary lymph nodes (LN) by qPCR were carried out. Only HIMC vaccinated goats showed a significant reduction of lung lesions volume and mycobacterial DNA load in LN compared to unvaccinated controls. Both vaccinated groups showed also a significant reduction of the other pathological parameters, an improved clinical outcome and a higher proportion of IFN-γ-producing central memory T cells after vaccination. The results indicated that homologous vaccination of goats with HIMC induced enhanced protection against M. caprae challenge by reducing lung pathology and bacterial load compared to the heterologous vaccine (HIMB). Further large-scale trials are necessary to assess the efficacy of autovaccines under field conditions.

show abstract

“…Amplification was performed in a 7500 fast real-time PCR system (Applied Biosystems, Walham, MA, USA). CFU genomic equivalents were calculated as previously described 44 .…”

Section: Multi-cytokine Quantification Assaymentioning

confidence: 99%

A proof-of-concept study to investigate the efficacy of heat-inactivated autovaccines in Mycobacterium caprae experimentally challenged goats

Melgarejo

Planas

Cobos

et al. 2022

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…The amplification was performed in a 7500 fast real-time PCR system (Applied Biosystems, Waltham, MA, USA). The genomic equivalents of CFU were calculated as previously described (Vidal et al, 2022 ).…”

Section: Methodsmentioning

confidence: 99%

Comparison of the pathological outcome and disease progression of two Mycobacterium caprae experimental challenge models in goats: endobronchial inoculation vs. intranasal nebulization

et al. 2023

Self Cite

View full text Add to dashboard Cite

BackgroundGoats are natural hosts of tuberculosis (TB) and are a valid animal model to test new vaccines and treatments to control this disease. In this study, a new experimental model of TB in goats based on the intranasal nebulization of Mycobacterium caprae was assessed in comparison with the endobronchial route of infection.MethodsFourteen animals were divided into two groups of seven and challenged through the endobronchial (EB) and intranasal (IN) routes, respectively. Clinical signs, rectal temperature, body weight, and immunological responses from blood samples were followed up throughout the experiment. All goats were euthanized at 9 weeks post-challenge. Gross pathological examination, analysis of lung lesions using computed tomography, and bacterial load quantification in pulmonary lymph nodes (LNs) by qPCR were carried out.ResultsThe IN-challenged group showed a slower progression of the infection: delayed clinical signs (body weight gain reduction, peak of temperature, and apparition of other TB signs) and delayed immunological responses (IFN-γ peak response and seroconversion). At the end of the experiment, the IN group also showed significantly lower severity and dissemination of lung lesions, lower mycobacterial DNA load and volume of lesions in pulmonary LN, and higher involvement of the nasopharyngeal cavity and volume of the lesions in the retropharyngeal LN.ConclusionThe results indicated that the IN challenge with M. caprae induced pathological features of natural TB in the lungs, respiratory LN, and extrapulmonary organs but extremely exaggerating the nasopharyngeal TB pathological features. On the other hand, the EB route oversized and accelerated the pulmonary TB lesion progression. Our results highlight the need to refine the inoculation routes in the interest of faithfully reproducing the natural TB infection when evaluating new vaccines or treatments against the disease.

show abstract

Lack of detection of Mycobacterium microti infection in wild rodents from a free-ranging wild boar outbreak area

Vidal,

Espunyes,

Ribas

et al. 2023

Eur J Wildl Res

View full text Add to dashboard Cite

Wild small rodents are considered the natural reservoirs of Mycobacterium microti, a member of the Mycobacterium tuberculosis complex (MTBC) that can cause tuberculosis (TB) in humans and animals, as well as interfere with current tuberculosis eradication plans in livestock. A cross-sectional study was carried out in the Catalan Pyrenees (Iberian Peninsula) in an area where M. microti was previously isolated from wild boars, to evaluate the role of micromammals in the epidemiology of this outbreak. A total of 350 wild rodents were necropsied (306 Murinae and 44 Arvicolinae) in spring and autumn during two consecutive natural years. Tissues were analyzed by histopathology to look for TB-like lesions and by qPCR and culture to detect MTBC. Sera were analyzed by MTBC-specific ELISA. No evidence of TB infection in wild rodents was confirmed. Results suggest that small rodents did not play a role in the epidemiology of M. microti in the area. The source of this mycobacterium remains unknown, but previous detections of M. microti in various species in southern France suggest the movements of wild boars across the French Pyrenees as the most likely origin of the outbreak detected in the Iberian Peninsula.

show abstract

Experimental Mycobacterium microti Infection in Bank Voles (Myodes glareolus)

Cited by 4 publications

References 30 publications

A proof-of-concept study to investigate the efficacy of heat-inactivated autovaccines in Mycobacterium caprae experimentally challenged goats

A proof-of-concept study to investigate the efficacy of heat-inactivated autovaccines in Mycobacterium caprae experimentally challenged goats

Comparison of the pathological outcome and disease progression of two Mycobacterium caprae experimental challenge models in goats: endobronchial inoculation vs. intranasal nebulization

Lack of detection of Mycobacterium microti infection in wild rodents from a free-ranging wild boar outbreak area

Contact Info

Product

Resources

About