Experimental murine candidiasis: pathological and immune responses to cutaneous inoculation with Candida albicans

Abstract

Cutaneous infection of mice with Candida albicans elicited a predominantly acute inflammatory response, stimulated the production of precipitating antibodies, and conferred protection against subsequent intravenous challenge with the same organism. The acute inflammatory skin reaction seen after cutaneous infection suggested a predominantly humoral response to Candida. Animals infected cutaneously a second time with viable C. albicans developed larger skin lesions than animals infected only once, and the twice-infected animals were more resistant to an intravenous challenge as well. The cutaneous inoculation of mice with heat-killed C. albicans was less effective in stimulating antibody production, in eliciting the inflammatory response, and in inducing a protective response demonstrable by intravenous challenge with viable Candida. This model of experimental candidiasis represents a reproducible means of studying a protective immune response to the organism. times with nonpyrogenic physiological sodium chloride (NPS) (Cutter Laboratories, Berkeley, Calif.). More than 95% of the cells in such suspensions were blastospores. The cells were then quantitated in a hemacytometer, and the counts were verified by diluting and plating the same suspensions with which animals were inoculated. Killed cells for animal inocula-499 on July 16, 2020 by guest http://iai.asm.org/ Downloaded from on July 16, 2020 by guest http://iai.asm.org/ Downloaded from

Experimental Murine Candidiasis: Pathological and Immune Responses in T-Lymphocyte-Depleted Mice

“…In our study, we chose a different model of Tlymphocyte deprivation, viz., mice thymectomized, irradiated, and bone marrow reconstituted as adults, to investigate the interaction between immunologically modified animals and C. albicans, and we chose to investigate not only their response to a first exposure to C. albicans, but also to reinfection. These studies, in fact, are based on an experimental model of murine candidiasis defined in normal, i.e., immunologically unmodified, mice (13,14), and involve cutaneous inoculation with viable C. albicans, intravenous challenges, and tests for humoral and cellular responses at various times after cutaneous inoculation. In those studies, resistance to reinfection, antibodies, and delayed hypersensitivity were demonstrable after cutaneous inoculation.…”

“…C. albicans B311, serotype A, obtained from H. Hasen-729 on July 5, 2020 by guest http://iai.asm.org/ Downloaded from clever, was maintained by monthly transfer on glucose-peptone agar slants and stored at 40C. Since specific cultural and fractionation techniques have been reported in detail elsewhere (13,14), they are described only briefly here. Viable blastospores for inoculation into mice were grown in a soy dialysate broth (27).…”

“…on July 5, 2020 by guest http://iai.asm.org/ Downloaded from tion after the initiation of infection have been described in detail elsewhere (13,14). Briefly, animals were infected once or twice cutaneously with 5.0 x 10" viable C. albicans blastospores, 2 weeks apart, and subsequently challenged intravenously with 1.0 x 10W viable blastospores.…”

“…Feet were measured with dial-type calipers (Schnelltaster, H. Krbplein GMBH, Schluechtern, Hesse, West Germany) before and after inoculation with antigen, and the net increase was determined. Antibodies were assayed by counterimmunoelectrophoresis (15,26) with a soluble cytoplasmic antigen (14) and by a whole-cell agglutination technique (34,36).…”

Entzündungsreaktion auf orale Candida-Exposition bei euthymischen und genetisch athymischen (nude) Mäusen: Inflammatory Reactions Due to Oral Candida-Exposure in Euthymic and Athymic (nude) Mice

“…This conception is in accord with clinical observations. Als Abwehrmechanismen bei Candidose von Haut und Schleimhauten ist neben epithelialen Veranderungen, die in einer Hyperkeratose, Akanthose und Parakeratose des Epithels (Cawson 1966;Russell und Jones 1975) sowie in einer vermehrten Epithelproliferation (Sohnle und Kirkpatrick 1978), einer Zunahme interepithelialer Langerhanszellen (Bos und Burkhardt 1977;) sowie in einer granulozytaren Infiltration des Epithels mit Ausbildung von Mikroabszessen mit perifokalem Udem bestehen (Ray und Wuepper 1976;Giger et al 1978;, auch eine subepitheliale Entziindungsreaktion von Bedeutung.…”