Experimental Models of Partial Intestinal Obstruction in Young Mice: Establishment, and Evaluation

Georgopoulos, Ioannis; Mavrigiannaki, Eleftheria; Stasinopoulou, Sotiria; Renieris, Georgios; Nikolakis, Georgios; Chaniotakis, Ioannis; Tiniakos, Dina; Papaconstantinou, Ioannis

doi:10.1016/j.jss.2020.03.007

Cited by 4 publications

(14 citation statements)

References 20 publications

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“…In our case though, a reverse connection between the inflammation and muscular hypertrophy is evident. Chronic partial obstruction induces pre-stenotic muscle hypertrophy [25,33,34]. Obstruction was not sufficient per se to induce inflammatory responses, as we did not observe substantial postoperational inflammation in WT mice.…”

Section: Discussionmentioning

confidence: 54%

“…In the present study we propose a novel animal model that it is suitable to study obstruction-inflammation interactions and their contribution to CD pathogenesis. This model combines the TNF Δare/+ genetic knockout mouse model, that is predisposed to Crohn-like inflammatory bowel disease [5] and a long-lasting partial obstruction technique [25]. The surgically partial obstruction was performed at 6 weeks of mice 14 14 age, a timepoint preceding the expected onset of ileitis in the TNF Δare/+ mouse model (i.e.…”

Section: Discussionmentioning

confidence: 99%

“…Throughout operation clean conditions were used. [23,24] In triple suture and triple suture WT groups, a partial intestinal obstruction was created via the triple suture intestinal stenosis technique of Georgopoulos et al [25] 3-4 cm cranially to the ileocecal valve. A non-absorbable suture 7-0 (Surgipro II, Covidien, USA) was passed transmurally through the lumen, on its antimesenteric border and was tied penetrating the one third of the lumen.…”

Section: Surgical Proceduresmentioning

confidence: 99%

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Experimental Intestinal Stenosis Alters Crohn’s Disease-Like Intestinal Inflammation in Ileitis-Prone Mice

et al. 2021

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Background: Clinical observations indicate that mechanical factors contribute to the expression or recurrence of Crohn's disease. We investigated whether the creation of an intestinal stenosis could alter the severity of the expected Crohn-like ileitis, in a Crohn's disease animal model, the TNF Δare/+ mouse. Methods: Thirty-six, 6-weeks-old TNF Δare/+ mice, were divided into 3 intervention groups: triple suture, single suture and sham. In the terminal ileum, in the first group, a triple suture stenosis was created, whereas, in the second, a loose suture was placed. Same triple-suture stenosis was performed on twelve wild type mice. All animals were sacrificed at 6 weeks post-operatively and the ileum parts were evaluated histopathologically. A summative total ileitis score was applied in each sample using a bespoke semiquantitative histological scoring system for the Crohn-like changes. Results: The triple suture stenosis induced significant muscular hypertrophy proximal to interventional site which was more prominent in TNF Δare/+ than wild type mice. In triple suture group, the total ileitis score was significantly increased proximal to the intervention as compared to the single suture ( P : 0.004) and the sham groups ( P : 0.013). The total ileitis score distally, was unaffected, regardless of the experimental intervention. Intestinal stenosis did not induce intestinal inflammation in WT mice. Conclusions: The creation of a stenosis in the terminal ileum of TNF Δare/+ mice alters Crohn-like inflammation. We assume that mechanical forces, such as intraluminal pressure, may contribute as important co-factors to the pathophysiology of Crohn's disease in genetically predisposed subjects.

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Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Section: Surgical Proceduresmentioning

confidence: 99%

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Experimental Intestinal Stenosis Alters Crohn’s Disease-Like Intestinal Inflammation in Ileitis-Prone Mice

et al. 2021

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“…Twelve male C57BL/6 mice aged 8 weeks were divided into two groups: Control (Ctrl, n = 4) and PSIO ( n = 8). All mice underwent surgery for partial intestinal obstruction as previously described ( Georgopoulos et al, 2020 ). Anesthesia was induced with an intraperitoneal ketamine injection (70 mg/kg, Xylapan, Vetoquinol, France), and the skin was cleaned with 70% ethanol.…”

Section: Methodsmentioning

confidence: 99%

Alterations of gut microbiota in a mouse model with partial small intestinal obstruction

Wang,

Zhang,

Jiang

et al. 2023

Front. Microbiol.

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IntroductionChanges in the gut microbiota of patients with partial small intestinal obstruction (PSIO) have not been widely clarified. We aimed to explore bacterial diversity in a PSIO mouse model.MethodsA PSIO mouse model was established using male C57BL/6 mice, and feces samples from the distal ileum and ileum epithelium tissues were collected. MiSeq sequencing of the 16S rRNA gene was conducted to characterize microbiota diversity and composition. RNA sequencing for differences in transcriptomic programming of the ileum tissue was performed between the PSIO and (Control) Ctrl groups.ResultsBacterial diversity in the PSIO group was significantly lower than that in the controls. Pseudomonadota was predominant in the feces of the PSIO group. Unclassified_Muribaculaceae (p = 0.008) and Akkermansia (p = 0.007) were more abundant in the Ctrl group than those in the PSIO group. Furthermore, Escherichia_Shigella (p = 0.008) was more predominant in the feces of the PSIO group. The Kyoto Encyclopedia of Genes and Genomes pathways related to metabolism were depleted in the PSIO group. Pathways associated with intestinal fibrosis, including extracellular matrix-receptor interaction, focal adhesion, phosphoinositide 3-kinase (PI3K)-Akt signaling pathway and transforming growth factor (TGF)-beta signaling pathway, which were enriched in ileum epithelial tissue in the PSIO group.ConclusionPSIO can lead to changes in the predominant intestinal bacterial groups. Depleted functional profiles of the gut microbiota were identified in the PSIO group. Functional pathways associated with intestinal fibrosis were activated by PSIO. The potential regulation by the microbiota needs to be explored in the future.

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“…To explore this phenomenon, Georgopoulos et al [7] in this issue of Digestive Diseases and Sciences created an experimental intestinal ileal stenosis through an intestinal suturing method [8] in the well-characterized TNFΔARE spontaneous ileitis model. The experiments were controlled by a sham operation with a suture not causing stenosis, as well as a sham operation with no suture.…”

mentioning

confidence: 99%

No Longer Stretching Credibility: Mechanical Force Meets Inflammation in Experimental Intestinal Stenosis

Steiner

Rieder

2021

Dig Dis Sci

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Experimental Models of Partial Intestinal Obstruction in Young Mice: Establishment, and Evaluation

Cited by 4 publications

References 20 publications

Experimental Intestinal Stenosis Alters Crohn’s Disease-Like Intestinal Inflammation in Ileitis-Prone Mice

Experimental Intestinal Stenosis Alters Crohn’s Disease-Like Intestinal Inflammation in Ileitis-Prone Mice

Alterations of gut microbiota in a mouse model with partial small intestinal obstruction

No Longer Stretching Credibility: Mechanical Force Meets Inflammation in Experimental Intestinal Stenosis

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