Experimental models of metabolic syndrome in rats

Gancheva, Silvia; Zhelyazkova-Savova, Maria; Galunska, Bistra; Chervenkov, Trifon

doi:10.14748/ssm.v47i2.1145

Cited by 32 publications

(27 citation statements)

References 31 publications

(34 reference statements)

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“…Our results were in line with other literature reports, whereby the consumption of HCHF diet decreased rates of glucose clearance in MetS rats and those animals were experiencing dyslipidemia [21,[25][26][27][28]. Apart from HCHF diet, an excess of dietary fructose [37,38], fat [39,40] or combination of fructose and fat [41,42] has been widely reported to contribute for insulin resistance and dyslipidemia.…”

Section: Discussionsupporting

confidence: 93%

The Effects of a Modified High-carbohydrate High-fat Diet on Metabolic Syndrome Parameters in Male Rats

Wong

Chin

Suhaimi

et al. 2017

Exp Clin Endocrinol Diabetes

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Metabolic syndrome is a cluster of metabolic abnormalities including central obesity, hyperglycemia, hypertension, and dyslipidemia. A previous study has established that high-carbohydrate high-fat diet (HCHF) can induce MetS in rats. In this study, we modified components of the diet so that it resembled the diet of Southeast Asians. This study aimed to determine the effects of this modified HCHF diet on metabolic parameters in rats. Male Wistar rats (n=14) were randomised into two groups. The normal group was given standard rat chow. The MetS group was given the HCHF diet, comprises of fructose, sweetened condensed milk, ghee, Hubble Mendel and Wakeman salt mixture, and powdered rat food. The diet regimen was assigned for a period of 16 weeks. Metabolic syndrome parameters (abdominal circumference, blood glucose, blood pressure, and lipid profile) were measured at week 0, 8, 12, and 16 of the study. The measurement of whole body composition (fat mass, lean mass, and percentage of fat) was performed using dual-energy X-ray absorptiometry at week 0, 8, and 16. Our results indicated that the components of MetS were partially developed after 8 weeks of HCHF diet. Systolic blood pressure, triglyceride, low density lipoprotein cholesterol, fat content, and percentage of fat was significantly higher in the HCHF group compared to normal group (p<0.05). After 12 weeks of HCHF diet, the rats showed significant increases in abdominal circumference, blood pressure, glucose intolerance, and dyslipidemia compared to normal control (p<0.05). In conclusion, MetS is successfully established in male rats induced by the modified HCHF diet after 12 weeks.

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Section: Discussionsupporting

confidence: 93%

The Effects of a Modified High-carbohydrate High-fat Diet on Metabolic Syndrome Parameters in Male Rats

Wong

Chin

Suhaimi

et al. 2017

Exp Clin Endocrinol Diabetes

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“…Feeding HFHF diet given rise to a significant increase in the serum levels of blood glucose, total lipids, total cholesterol, VLDL-c, LDL-c and TAG with a significant reduction in both serum insulin and HDL-c which confirms the state of T2DM. Gancheva et al [29] and López et al [45] reported that, feeding HFHF diet for 12 weeks resulted in induction of metabolic syndrome and with elevated serum glucose and lipid profile and a reduction in serum insulin and HDL-c.…”

Section: Discussionmentioning

confidence: 99%

“…The caloric intake would be 800 kcal per 100 g food, of which 60% provided by the lard and 17% by the fructose supplementary to the typical rat foodstuff for one month. After that, the blood glucose level was measured [29], and rats with blood glucose more than 200mg/dl were taken as obese-diabetic group whereas that less than 200mg/dl considered as obese non-diabetic group. The rats were then subdivided into different groups and received different treatments for 4 weeks as follow: Group 2 (G2): obese non-diabetic didn't take any medication,group 3 (G3): obese-diabetic didn't take any medication.…”

Section: Experimental Designmentioning

confidence: 99%

Characterization of Circulating Exosomal MicroRNAs and Its Role as Biomarkers for Metabolic Syndrome

El-Dawy

Ali

Abdo

2019

Zagazig Veterinary Journal

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Exosomal microRNAs (EXO-miRNAs) attract major importance as prospective diagnostic biomarkers for metabolic syndrome (MS). The intent of our trials was to characterize the serum exosomes, exosomal miRNA and their target genes to identify those that were altered in relation to the different conditions of metabolic syndrome (Type II diabetes mellitus, high-fat-induced obesity and its related disorders) with different forms of treatment to evaluate their use as diagnostic and treatment biomarkers. Eighty male adult albino rats were used in this experiment, 10 served as normal control group and other 70 divided into 7 different groups; the first received high fat high fructose (HFHF) diet for 4 weeks, second received HFHF diet and confirmed to be diabetic and the other five groups are diabetics and treated for another 4 weeks with Okra extract, Gum Arabic, Dapagliflozin, combination of Dapagliflozin& okra Extract and combination of Dapagliflozin& Gum Arabic, respectively.. Our results explained that, the expression levels of miR-122, fatty acid synthase-1 (FAS-1) and Sterol response elementary binding protein-1 (SREBP-1) were significantly higher in the hepatic tissue of metabolic syndrome model groups than that of control and significantly down-regulated after different treatments but still higher than that of control group while carnitine palmitoyltransferase-1(CPT-1) was significantly decreased in the same groups. Also the expression levels of miR-31 was significantly higher in adipose tissue of metabolic syndrome model groups than that of control and significantly down-regulated after different treatments but still higher than that of control group while Leptin gene (Ob), Phosphoinositide-3-kinase, class 2, alpha-polypeptide (PIK3C2A) and Peroxisome proliferator-activated receptor-γ (PPAR-γ) were significantly decreased in the same groups.It was concluded that exosomal miRNA signatures are parallel with pathological consequences of metabolic syndrome sufferers as well as numerous miRNAs display potential biomarkers for non-invasive diagnosis of the metabolic changes.

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“…This model was planned to mimic nearly the cafeteria diets (CAF) which essentially contain high caloric value [5]. The body weight and blood glucose were taken once weekly for 12 wk [9].…”

Section: Formulation Of Hfhf Dietmentioning

confidence: 99%

“…MicroRNA-146a is involved in the pathogenesis of autoimmune diseases and several types of cancer [3]. In addition, miRNA-146a can be involved in the pathology of inflammatory degenerative diseases in human, such as prion disease [6], Alzheimer's disease [7,8] and epilepsy [9]. In ischemic stroke the increased microRNA-126 in endothelial cells can promote angiogenesis, and attenuate vascular dysfunction in diabetics [10].…”

Section: Introductionmentioning

confidence: 99%

Micro RNA in Degenerative Disorders

naggar

2019

Zagazig Veterinary Journal

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Obesity and type 2 diabetes mellitus are considered as the most risk criteria in metabolic syndrome. Twenty four 3 months old male albino rats, weighting (120±15) gm were purchased from animal house at the Faculty of Veterinary Medicine, Zagazig University, Egypt. After acclimatization at 23-25°C, 12 h light-dark cycle and free availability of water and diet, rats were allocated into 2 main groups. The first group (G1) was a control group (8 rats) that was kept on a standard diet with free access to drinking water. The second group (G2) was consisted of 16 rats that were kept on high fat high fructose diet (HFHF), which then was subdivided into 2 equal subgroups, G2.1 and G2.2. Subgroup G2.1 was kept for 7 wk (Obese animals). Subgroup G2.2 was kept for 12 wk (Type II diabetic animals). Feeding HFHF diet resulted in marked increase in body weight in both obese and diabetic groups, then started to decrease and stopped between 7th and 8th wk till the end of the experiment. Moreover, HFHF diet decreased the expression levels of insulin hormone gene which was reflected on the increase in the expression levels of fibroblast growth factor 21 (FGF-21), interleukin 1 beta (IL-1β), interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-α) genes. The highest level of IL-1β was recorded in the diabetic animals (G2.2). While the maximum FGF-21 gene detection in obese group (G2.1). On the other hand, IL-6 and TNF-α equally increased in both groups. Hitopathologically, hepatic tissues reveled fatty change with pancreatic atrophy in both HFHF diet treated groups. The results showed that, feeding HFHF diet resulted in increasing the expression levels of regulatory miRNA 146a and miRNA 126 genes. It could be concluded that the expression levels of miRNA 146a, miRNA 126, FGF 21 and some cytokines may be a good early indicator for obesity and diabetes mellitus type II, having a major role in the mechanism of incidence and may have a great role in the treatment and follow-up the disease.

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Experimental models of metabolic syndrome in rats

Cited by 32 publications

References 31 publications

The Effects of a Modified High-carbohydrate High-fat Diet on Metabolic Syndrome Parameters in Male Rats

The Effects of a Modified High-carbohydrate High-fat Diet on Metabolic Syndrome Parameters in Male Rats

Characterization of Circulating Exosomal MicroRNAs and Its Role as Biomarkers for Metabolic Syndrome

Micro RNA in Degenerative Disorders

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