Experimental model of toxin-induced subclinical mastitis and its effect on disruption of follicular function in cows

Furman, Ori; Leitner, Gabriel; Roth, Zvi; Lavon, Y.; Jacoby, Shamay; Wolfenson, D.

doi:10.1016/j.theriogenology.2014.08.002

Cited by 20 publications

(18 citation statements)

References 45 publications

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“…This is associated with low expression of GDF-9, a germ-cell marker and member of the transforming growth factor b superfamily which plays a central role in folliculogenesis and oocyte maturation. In agreement, 3-wk induction of subclinical mastitis by multiple injections of low doses of Gþ toxin of S aureus origin attenuated the growth of medium-sized follicles [54]. In contrast to these studies, naturally occurring subclinical mastitis increased growth of medium-sized follicles, most likely due to decreased dominance of the large follicle, expressed by reduced steroid content in the follicular fluid [55].…”

Section: Effects Of Mastitissupporting

confidence: 61%

Comparing the effects of heat stress and mastitis on ovarian function in lactating cows: basic and applied aspects

Roth

Wolfenson

2016

Domestic Animal Endocrinology

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Section: Effects Of Mastitissupporting

confidence: 61%

Comparing the effects of heat stress and mastitis on ovarian function in lactating cows: basic and applied aspects

Roth

Wolfenson

2016

Domestic Animal Endocrinology

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“…However in this study serum estradiol concentrations were all higher in the subclinical mastitis group than the control cows and at 24 th and 48 th h and the differences were significant (P<0.05). Furman et al [22] have reported that experimental subclinical intramammary infection did not cause an immediate decline in follicular estradiol but rather a marked, delayed decline in estradiol concentrations 16 days after mastitis induction was terminated. Moreover large portion of cows (especially consisting of Gram positive group) were not affected by the administration and follicular estradiol levels remained normal.…”

Section: Discussionmentioning

confidence: 99%

“…These variations among cows were presumed to be related to individual, genetic differences. Authors have also suggested that the antral and medium sized follicles are more susceptible to stress and the effect of subclinical intrammammary infections (IMI) thus occurs belatedly [22] . In the present study as previously discussed above the mean follicular diameters of cows were higher than observed in the other studies and consequently larger follicles may be less affected from subclinical mastitis.…”

Section: Discussionmentioning

confidence: 99%

Effects of Subclinical Mastitis on Serum Estradiol and Tumour Necrosis Factor Alpha Levels During Estrus in Dairy Cows

Musal¹,

Türkyılmaz²,

Beceriklisoy³

et al. 2016

Kafkas Univ Vet Fak Derg

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The effect of subclinical mastitis on serum estradiol and tumor necrosis factor alpha (TNF-α) levels during oestrus and subsequent fertility was investigated in dairy cows. 40 cows were divided into two groups as healthy control (n=20) and subclinical mastitis (n=20), according to the results of California Mastitis Test (CMT) and bacteriological isolation and identification. Cows were synchronised with a standart 7 day Ovsynch protocol. Following prostaglandin F2alpha (PGF2α) administration, cows were examined with trans-rectal ultrasonography at 24, 36 and 48 th h, dominant follicle diameters were recorded and blood samples were collected. Sixteen h after the second gonadotrophin-releasing hormone (GnRH) administration, cows were inseminated and a final examination of ovaries were performed and dominant follicle diameters were recorded. estradiol and TNF-α concentrations were analysed with ELISA in serum samples. No significant differences were found between the follicular diameters and growth patterns (P>0.05) of the two groups while estradiol concentations were significantly higher in the subclinical mastitis group than the control group at 24 and 48 h after PGF2α injection (P=0.017 and P=0.036 respectively). Also TNF-α levels were significantly higher in cows with subclinical mastitis than the control group (P=0.03). Positive correlations were observed between estradiol and TNF-α levels, in both groups (Control Group: R=0.512, P=0.021; Subclinical Mastitis Group: R=0.826, P<0.001). Overall pregnancy rate was higher in the control group (40%) than the subclinical mastitis (25%) group however the difference was not statistically significant (P>0.05). In conclusion estradiol and TNF-α concentrations were found higher in cows with subclinical mastitis during estrus and this data may be due to a luteal insufficiency during the initiation of synchronization, however further studies are required. Özet Süt ineklerinde subklinik mastitislerin serum östradiol ve tümör nekrozis faktör alfa (TNF-α) düzeyleri ve fertilite üzerine etkileri araştırıldı. Kaliforniya Mastitis Test (CMT) ve bakteriyolojik izolasyon identifikasyon sonuçlarına göre 40 inek sağlıklı kontrol (n=20) ve subklinik mastitisli (n=20) olmak üzere iki gruba ayrıldı. İnekler standart bir 7 günlük Ovsynch protokolü ile senkronize edildi. Prostaglandin F2 alfa (PGF2α) uygulamasını takiben inekler 24, 36 ve 48. saatlerde transrektal ultrasonografi ile muayene edildi, dominant follikül çapları kaydedildi ve kan örnekleri toplandı. İkinci gonadotropin salgılatıcı hormon (GnRH) uygulamasını takiben 16. saatte inekler tohumlandı, son bir ovaryum muayenesi yapıldı ve dominant follikül çapları kaydedildi. Serum örneklerinde östradiol ve TNF-α düzeyleri ELISA ile belirlendi. Folikül çapları ve folliküler gelişim özelliği yönünden iki grup arasında önemli bir fark görülmezken (P>0.05), serum östradiol konsantrasyonları subklinik mastitis grubunda kontrol grubuna göre PGF2α enjeksiyonunu takiben 24 ve 48. saatlerde belirgin ölçüde yüksek bulundu (sırasıyla, P=0.017 ...

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“…The in vivo part of the study was shared with the study conducted by Furman et al (2014). It included intramammary administration of toxins mimicking subclinical mastitis over a period of 20 days.…”

Section: Experimental Designmentioning

confidence: 99%

“…Subclinical mastitis was induced as described previously by Furman et al (2014). Briefly, healthy Holstein cows (n ¼ 22; somatic cell count (SCC) ,200 000) were synchronised by the 'Ovsynch' protocol and randomly separated into three groups.…”

Section: In Vivo Proceduresmentioning

confidence: 99%

Subclinical mastitis disrupts oocyte cytoplasmic maturation in association with reduced developmental competence and impaired gene expression in preimplantation bovine embryos

Roth¹,

Asaf²,

Furman³

et al. 2016

Reprod. Fertil. Dev.

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Subclinical chronic mastitis was induced to examine the effects on oocyte developmental competence. Uninfected Holstein cows were intramammary administrated with serial (every 48h for 20 days) low doses of toxin of Staphylococcus aureus origin (Gram-positive; G+), endotoxin of Escherichia coli origin (Gram-negative; G-) or sterile saline (control). Follicular fluid of toxin- and saline-treated cows was aspirated from preovulatory follicles and used as maturation medium. Oocytes harvested from ovaries collected at the abattoir were matured and then fertilised and cultured for 8 days. The percentage of oocytes undergoing nuclear maturation, determined by meiotic nuclear stages, did not differ between groups. Cytoplasmic maturation, determined by cortical granule distribution, was affected by both toxins (PPPPTGS2) mRNA increased, whereas that of growth differentiation factor 9 (GDF9) decreased in matured oocytes. In addition, PTGS2 expression increased and POU class 5 homeobox 1 (POU5F1) expression decreased in 4-cell embryos developed from both G+ and G- oocytes. Thus, regardless of toxin type, subclinical mastitis disrupts oocyte cytoplasmic maturation and alters gene expression in association with reduced developmental competence.

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Experimental model of toxin-induced subclinical mastitis and its effect on disruption of follicular function in cows

Cited by 20 publications

References 45 publications

Comparing the effects of heat stress and mastitis on ovarian function in lactating cows: basic and applied aspects

Comparing the effects of heat stress and mastitis on ovarian function in lactating cows: basic and applied aspects

Effects of Subclinical Mastitis on Serum Estradiol and Tumour Necrosis Factor Alpha Levels During Estrus in Dairy Cows

Subclinical mastitis disrupts oocyte cytoplasmic maturation in association with reduced developmental competence and impaired gene expression in preimplantation bovine embryos

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