Experimental Model of Peritoneal Adhesion

Zhura, Alexandr; Tratsyak, Stanislau; Khryshchanovich, V.J.; Makarevich, Z.A.

doi:10.18484/2305-0047.2017.4.333

Cited by 6 publications

(3 citation statements)

References 4 publications

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“…The evaluation of biocompatibility, biodegradation and potential anti- To study the biological properties of the obtained porous scaffolds, an experimental model of peritoneal adhesions, which had been developed earlier, was used (Zhura, Tretyak, & Khryshchanovich, 2017). Under general anesthesia by Thiopentalum-natrium, laboratory rats were subjected to laparotomy and 2 cm defect of the parietal peritoneum of the lateral abdominal wall was created.…”

Section: In Vivo Studymentioning

confidence: 99%

Fabrication and characterization of pectin‐based three‐dimensional porous scaffolds suitable for treatment of peritoneal adhesions

Kulikouskaya

Kraskouski

Hileuskaya

et al. 2019

J Biomedical Materials Res

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Formation of peritoneal adhesions is common complication after abdominal and pelvic surgery. They bear a significant health problem with an influence to quality of life and health care expenses. Promising approach for their prevention is using of biodegradable barrier films for physical separation of peritoneal surfaces. In the present study, highly porous pectin‐based three‐dimensional (3D) scaffolds were obtained by freeze‐drying technique. Physico‐chemical properties of the formed materials, including their morphology, porosity, density, and stability, have been studied. The evaluation of their biocompatibility, biodegradation, and potential antiadhesion effect was studied by in vivo experiment. To reinforce the scaffolds structure and improve their stability in physiological solutions, pectin chains were cross‐linked with divalent cations. We determined optimal cross‐linking conditions, which allow obtaining scaffolds with desired biodegradation rate. These cross‐linked scaffolds fully dissolved within 8 days in the peritoneal cavity with low presence of complications and some antiadhesive effect. It has also been determined that mesenchymal stem cells from adipose tissue could effectively adhere to the scaffolds with preservation of their viability. Our results show that obtained materials can be suggested as mechanical scaffold for delivery of the stem cells culture to peritoneal surfaces as a part of complex antiadhesive barrier system.

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Section: In Vivo Studymentioning

confidence: 99%

Fabrication and characterization of pectin‐based three‐dimensional porous scaffolds suitable for treatment of peritoneal adhesions

Kulikouskaya

Kraskouski

Hileuskaya

et al. 2019

J Biomedical Materials Res

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“…Попередити утворення спайок можна лише у перші кілька діб, й одним з можливих механізмів цієї профілактики є активування місцевих механізмів фібринолізу. Пошук методів активації фібринолітичної системи ведеться давно, запропоновано різні методики, які не продемонстрували стовідсоткової ефективності [11].…”

Section: профилактика развития перитонеальных тазовых спаек у женщин репродуктивного возраста тг романенко пф шагановunclassified

Prevention of peritoneal pelvic adhesion formation in women of reproductive age

Romanenko

Shaganov

2021

RHW

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The objective: a prevention of the peritoneal pelvic adhesions formation in women of reproductive age in the postoperative periodMaterials and methods. 132 women with tubal infertility were examined: І group – main one (n=68) and ІІ group – comparison group (n=64). In the postoperative period, the patients of the main group received treatment and prevention measures according to the developed approach (intraoperative administration of anti-adhesion gel 50 ml and 1 suppository of the tiotriazolin a day rectally from the 1st day after surgery for 14 days), and the comparison group – according to the traditional approach. 30 healthy women were examined as a control group.The level of fibrinogen, fibrinogen /fibrin degradation products (FDP), protein-bound hydroxyproline and free hydroxyproline were studied in blood. Pain syndrome was assessed on a 10-point scale.Results. An increase in fibrinogen and FDP levels in the plasma of all examined women were found. But in the main group the concentration of FDP from 4 hours after intervention was significantly higher (5.31±0.24 mg/l) compared to the comparison group (4.53±0.17 mg/l). The level of protein-bound hydroxyproline in the blood serum of the examined women demonstrated its significant increase in the comparison group 12 hours after surgery, with a maximum after 24 hours and maintaining the concentration in 1.9 times higher after 3 days (p<0.05). The level of free hydroxyproline in the main group was significantly (in 1.4–1.6 times) higher than in the comparison group.The increase of the fibrinolytic capacity (p<0.05) in the peritoneal fluid was found during postoperative period. The period of hyperemia in II group lasted 2 times longer than in I group. Also, II group predominates in the number of patients with hyperthermia for more than 3 days – 10 (15.6 %), compared with I group – 4 patients (5.8 %). The duration of stay in the hospital dominated in II group with a standard treatment regimen in the postoperative period – 3.1 %, compared with I group – 1.9 %.The patients after operation in I group evaluated postoperative pain on average by 1.8 points (p=0.00), in II group – at 3.6 points (p=0.06). Synthetic opioid analgesics and nonsteroidal anti-inflammatory drugs were administered intramuscularly for analgesia. The patients in II group required more injections per patient.Conclusions. The proposed approach is effective, pathogenetically reasonable and can be used as a pathogenetic prevention of adhesions formation in women of reproductive age.

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“…Затем в эксперименте на лабораторных крысах in vivo оценивали биосовместимость, биодеградируемость и противоспаечное действие полученных материалов. Для изучения биологических свойств применяли разработанную ранее экспериментальную модель перитонеальных спаек [8]. Под общей анестезией лабораторным крысам выполняли лапаротомию и формировали дефект париетальной брюшины боковой стенки живота диаметром 2 см.…”

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Application of bioderadable materials based on alginate and pectin to prevent the formation of peritoneal adhesions

Zhura

Куликовская

Гилевская

et al. 2019

Vescì Nac. akad. navuk Belarusì, Ser. med. navuk

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Treatment of peritoneal adhesions are still of great importance today. One of the prophylactic measures is biodegradable gels and membranes. The objective of the investigation was to develop and to experimentally assess new materials based on pectin and alginate. Alginate hydrogel was prepared with 4.0, 7.0 and 10.0 weight per cent. The pectin sols were synthesized by the “green chemistry” method. To make flms and porous membranes the solution casting method and the freeze-drying technique were used accordingly. The materials were studied in vitro and in vivo. Their physical properties, biocompatibility, biodegradability, adhesion, the prevention effect, the possibility of using as a matrix for mesenchymal stem cell transplantation were assessed. Alginate hydrogel of 7.0 weight per cent didn’t cause postoperative complications and led to low adhesions incidence – in 10 % of cases (in the comparison group – 85.7 %). Pectin flms obtained by the solution casting method became deformed already in the physiological solution. Biodegradation of the flms was absent in the experiment, abscesses and infltrates in the abdominal cavity were noted. Mesenchymal stem cells didn’t attach to such flms. Porous pectin matrices synthetized by the freeze-drying technique became partially decomposed already in the physiological solution. In the experiment, these membranes were biodegraded in half animals with the formation of mild adhesions only in 25 %. Mesenchymal stem cells showed a good attachment to their surface. The developed materials based on alginate gel and porous pectin membranes showed a high biodegradation, good biocompatibility, adhesion the prevention effect and the possibility of using as a matrix for stem cells transplantation.

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Experimental Model of Peritoneal Adhesion

Cited by 6 publications

References 4 publications

Fabrication and characterization of pectin‐based three‐dimensional porous scaffolds suitable for treatment of peritoneal adhesions

Fabrication and characterization of pectin‐based three‐dimensional porous scaffolds suitable for treatment of peritoneal adhesions

Prevention of peritoneal pelvic adhesion formation in women of reproductive age

Application of bioderadable materials based on alginate and pectin to prevent the formation of peritoneal adhesions

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