Experimental Model of Ocular Hypertension in the Rat: Study of the Optic Nerve Capillaries and Action of Hypotensive Drugs

Díaz, F.; Villena, Alicia; Vidal, Lourdes; Moreno, Mercedes; García-Campos, J.M.; Vargas, I. Pérez de

doi:10.1167/iovs.09-3667

Cited by 10 publications

(17 citation statements)

References 37 publications

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“…This finding implicates that the correlation between the extent of RGC loss and the duration of IOP elevation exists. Our result was similar to that of the previous studies . RGC loss were 36.4 ± 2.4% and 23.1 ± 2.1% in the models using magnetic microspheres and latex microspheres injection into the anterior chamber, respectively .…”

Section: Discussionsupporting

confidence: 92%

“…26 An episcleral vein cautery model showed RGC loss of 30-40%. 27,28 Our study has several limitations. First, the number of rats used in our study is relatively small.…”

Section: Discussionmentioning

confidence: 94%

“…Our result was similar to that of the previous studies. 11,12,[26][27][28] RGC loss were 36.4 Ϯ 2.4% and 23.1 Ϯ 2.1% in the models using magnetic microspheres and latex microspheres injection into the anterior chamber, respectively. 11,12 Injecting hypertonic saline into aqueous veins of the rats produced RGC loss of 33%.…”

Section: Discussionmentioning

confidence: 94%

“…Injecting hypertonic saline into aqueous veins of the rats produced RGC loss of 33% . An episcleral vein cautery model showed RGC loss of 30–40% …”

Section: Discussionmentioning

confidence: 99%

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Experimental chronic ocular hypertension by anterior chamber injection of 0.3% carbomer solution in the rat

Kim

Park

2012

Clinical Exper Ophthalmology

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Section: Discussionsupporting

confidence: 92%

“…26 An episcleral vein cautery model showed RGC loss of 30-40%. 27,28 Our study has several limitations. First, the number of rats used in our study is relatively small.…”

Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 94%

“…Injecting hypertonic saline into aqueous veins of the rats produced RGC loss of 33% . An episcleral vein cautery model showed RGC loss of 30–40% …”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Experimental chronic ocular hypertension by anterior chamber injection of 0.3% carbomer solution in the rat

Kim

Park

2012

Clinical Exper Ophthalmology

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“…The effect of opioids on the organs of the immune system is insufficiently studied, particularly at primary lymphoid organs, the thymus. In the special literature there are data about influence on the structural organization of the thymus various harmful factors [5,6] such as indomethacin, histynat, methyl alcohol, naloxone, hyperosmolar solution [7], imunofan [8], cyclophosphamide [9][10][11] but there are only a few data on the impact of drugs in this important primary lymphoid organs. Application of imunofan has the pronounced effect on the maturation and differentiation of thymocytes in the thymus of rats of reproductive age [12].…”

Section: Introductionmentioning

confidence: 99%

The Features of Arterial Thymusatnalbuphineaction

Harapko

Holovatsky

2016

EUREKA: Health Sciences

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The article presents and analyzes the results of the pilot study, at which opioid nalbuphine was daily administered in white rats-males of reproductive age (1,5-3,5-month) for six weeks and one week after discontinuation of therapy. The drug "nalbuphine" was injected intraperitoneally daily 1 per day over 42 days. The data were compared with a control group of intact animals. The study was conducted on 52 animals, which were divided into eight groups. Morphometric study was performed using a system of visual analysis of histological preparations. Dynamics of changes in the external and internal diameters of medium caliber arteries thymus, media and lumen area, Vohenvorta ratio was studied. The increase of the external diameter and reduction of the internal diameter of the medium caliber arteries, increase of the area of media, increase of the area of the lumen, increase of the Vohenvortaratio.The blood vessels are dilated, gaps filled elements of the blood vessels around the swelling, the process of vascular sclerosis.

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A modified chronic ocular hypertension rat model for retinal ganglion cell neuroprotection

Zhong

2013

Front. Med.

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This study aimed to modify a chronic ocular hypertension (OHT) rat model to screen for potential compounds to protect retinal ganglion cells (RGCs) from responding to increased intraocular pressure (IOP). A total of 266 rats were prepared and randomly grouped according to different time-points, namely, weeks 3, 8, 16, and 24. Rats were sedated and eye examination was performed to score as the corneal damage on a scale of 1 to 4. The OHT rat model was created via the injection of a hypertonic saline solution into the episcleral veins once weekly for two weeks. OHT was identified when the IOP at week 0 was [Symbol: see text] 6 mmHg than that at week -2 for the same eye. Viable RGCs were labeled by injecting 4% FluoroGold. Rats were sacrificed, and the eyes were enucleated and fixed. The fixed retinas were dissected to prepare flat whole-mounts. The viable RGCs were visualized and imaged. The IOP (mean ± SD) was calculated, and data were analyzed by the paired t-test and one-way ANOVA. The OHT model was created in 234 of 266 rats (87.97%), whereas 32 rats (12.03%) were removed from the study because of the absence of IOP elevation (11.28%) and/or corneal damage scores over 4 (0.75%). IOP was elevated by as much as 81.35% for 24 weeks. The average IOP was (16.68 ± 0.98) mmHg in non-OHT eyes (n = 234), but was (27.95 ± 0.97) mmHg in OHTeyes (n = 234). Viable RGCs in the OHT eyes were significantly decreased in a time-dependent manner by 29.41%, 38.24%, 55.32%, and 59.30% at weeks 3, 8, 16, and 24, respectively, as compared to viable RGCs in the non-OHT eyes (P < 0.05). The OHT model was successfully created in 88% of the rats. The IOP in the OHT eyes was elevated by approximately 81% for 24 weeks. The number of viable RGCs was decreased by 59% of the rats in a time-dependent manner. The modified OHT model may provide an effective and reliable method for screening drugs to protect RGCs from glaucoma.

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Experimental Model of Ocular Hypertension in the Rat: Study of the Optic Nerve Capillaries and Action of Hypotensive Drugs

Cited by 10 publications

References 37 publications

Experimental chronic ocular hypertension by anterior chamber injection of 0.3% carbomer solution in the rat

Experimental chronic ocular hypertension by anterior chamber injection of 0.3% carbomer solution in the rat

The Features of Arterial Thymusatnalbuphineaction

A modified chronic ocular hypertension rat model for retinal ganglion cell neuroprotection

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