Experimental model of biofilm implant‐related osteomyelitis to test combination biomaterials using biofilms as initial inocula

Williams, Dustin L.; Haymond, Bryan S.; Woodbury, Kassie L.; Beck, James P.; Moore, David E.; Epperson, Richard T.; Bloebaum, Roy D.

doi:10.1002/jbm.a.34123

Cited by 40 publications

(44 citation statements)

References 47 publications

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“…First, our previously published studies have indicated that the MRSA isolate used in this study produces biofilms on PEEK material [19][20][21][22]. Second, PEEK is commonly used in spinal implant surgeries and these data may provide an indication of MRSA biofilm formation on PEEK materials that may be relevant to other clinical applications.…”

Section: Materials and Machiningmentioning

confidence: 88%

“…It was collected from the knee of an infected patient who underwent arthroscopic surgery and was kindly provided by ARUP Laboratories (Salt Lake City, UT, USA). This isolate has been confirmed to contain the icaADBC gene operon and has been used in previous studies to confirm its pathogenic and biofilm-forming properties [19,21,22]. The isolate was stored at À80°C and passaged fewer than two times on Columbia blood agar (Hardy Diagnostics, Midvale, UT, USA) before each use.…”

Section: Bacterial Isolatementioning

confidence: 99%

“…Using previously established protocols [19][20][21][22], after 48 hours, each sample was removed from a chamber of the flow cell, rinsed three times in phosphate-buffered saline, placed into 20 mL phosphate-buffered saline, vortexed for 1 minute, and sonicated at 42 kHz for 10 minutes.…”

Section: Biofilm Formation and Quantificationmentioning

confidence: 99%

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Does Vitamin E-blended UHMWPE Prevent Biofilm Formation?

Williams

John²,

Lerdahl

et al. 2015

Clinical Orthopaedics &Amp; Related Research

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Background Biofilm-related periprosthetic infections are catastrophic to patients and clinicians. Data suggest the addition of vitamin E to UHMWPE may have the ability to reduce biofilm formation on the surface of UHMWPE; however, previous studies were performed using stagnant broth solutions that may not have simulated a physiologic environment. In addition, the observed differences in levels of bacterial attachment, though statistically significant, may not be clinically significant. Questions/purposes We blended vitamin E with UHMWPE material and tested it for the ability to resist biofilm formation using a clinical isolate of methicillin-resistant Staphylococcus aureus (MRSA). Three additional materials were tested for comparison: highly crosslinked UHMWPE, compressionmolded UHMWPE, and polyetheretherketone. We also determined whether the surface roughness of these materials facilitated biofilm formation. Methods Using a flow cell system, samples of each material type were placed into separate chambers. A 10% solution of brain-heart infusion broth containing 10 5 colony-forming units (CFUs)/mL was flowed through the flow cell over 48 hours. The number of bacteria that adhered to the surface was quantified and biofilm formation was observed qualitatively using scanning electron microscopy. Optical profilometry was used to determine the surface roughness of each material type.Results Vitamin E-blended UHMWPE did not reduce biofilm formation of a clinically relevant strain of MRSA compared to materials that did not have vitamin E. More specifically, vitamin E-blended materials had similar amounts of biofilm formation (* 8 log 10 CFUs/cm 2 ) compared to materials not containing vitamin E (* 8.1 log 10 CFUs/cm 2 ) (p [ 0.4). The roughness of vitamin E-blended material surfaces (mean ± SD: 1.85 ± 0.46 lm) compared to that of materials without vitamin E (2.06 ± 1.24 lm) did not appear to influence biofilm formation. Conclusions Under physiologically relevant conditions, vitamin E-blended UHMWPE did not have the ability to reduce the formation of biofilms by MRSA. Clinical Relevance These data indicate that the addition of vitamin E to UHMWPE may not reduce clinically relevant rates of biofilm-related periprosthetic infections of total joint arthroplasty devices.

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Section: Materials and Machiningmentioning

confidence: 88%

Section: Bacterial Isolatementioning

confidence: 99%

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Does Vitamin E-blended UHMWPE Prevent Biofilm Formation?

Williams

John²,

Lerdahl

et al. 2015

Clinical Orthopaedics &Amp; Related Research

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“…Williams et al recently published a model simulating type IIIB Gustilo open fracture using the proximal part of the tibia of sheep (Williams et al , 2012b). They used two 2.0 × 2.0 cm metal plates separated by 1.0 cm that could accommodate a membrane that was coated with a MRSA biofilm.…”

Section: Literature Review Of Animal Modelsmentioning

confidence: 99%

A systematic review of animal models for Staphylococcus aureus osteomyelitis

Reizner¹,

Hunter²,

O’Malley³

et al. 2014

eCM

112

106

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Staphylococcus aureus (S. aureus) osteomyelitis is a significant complication for orthopaedic patients undergoing surgery, particularly with fracture fixation and arthroplasty. Given the difficulty in studying S. aureus infections in human subjects, animal models serve an integral role in exploring the pathogenesis of osteomyelitis, and aid in determining the efficacy of prophylactic and therapeutic treatments. Animal models should mimic the clinical scenarios seen in patients as closely as possible to permit the experimental results to be translated to the corresponding clinical care. To help understand existing animal models of S. aureus, we conducted a systematic search of PubMed and Ovid MEDLINE to identify in vivo animal experiments that have investigated the management of S. aureus osteomyelitis in the context of fractures and metallic implants. In this review, experimental studies are categorised by animal species and are further classified by the setting of the infection. Study methods are summarised and the relevant advantages and disadvantages of each species and model are discussed. While no ideal animal model exists, the understanding of a model's strengths and limitations should assist clinicians and researchers to appropriately select an animal model to translate the conclusions to the clinical setting.

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“…The in vitro data that were generated and the CSA-13 coating itself were translated to a sheep model of a simulated Type IIIB open fracture [52]. The method by which infection was caused in the sheep model of this study was to first grow well-established biofilms of MRSA in a membrane biofilm reactor [53,54].…”

Section: Introductionmentioning

confidence: 99%

In vivo efficacy of a silicone‒cationic steroid antimicrobial coating to prevent implant-related infection

et al. 2012

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Active release antimicrobial coatings for medical devices have been developed to prevent and treat biofilm implant-related infections. To date, only a handful of coatings have been put into clinical use, with limited success. In this study, a novel antimicrobial compound was incorporated into a silicone (polydimethylsiloxane or PDMS) polymer to develop a novel active release coating that addressed several limitations of current device coatings. The efficacy of this coating was optimized using an in vitro flow cells system, then translated to an animal model of a simulated Type IIIB open fracture wherein well-established biofilms were used as initial inocula. Results indicated that the novel coating was able to prevent infection in 100% (9/9) of animals that were treated with biofilms and the novel coating (treatment group). In contrast, 100% (9/9) of animals that were inoculated with biofilms and not treated with the coating (positive control), did develop infection. Nine animals were used as negative controls, i.e., those that were not treated with biofilms, and showed a rate of infection of 11% (1/9). Eight animals were treated with the novel coating only to determine its effect on host tissue. Results indicated that the novel active release coating may have significant promise for future application to prevent biofilm implant-related infections in patients.

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Experimental model of biofilm implant‐related osteomyelitis to test combination biomaterials using biofilms as initial inocula

Cited by 40 publications

References 47 publications

Does Vitamin E-blended UHMWPE Prevent Biofilm Formation?

Does Vitamin E-blended UHMWPE Prevent Biofilm Formation?

A systematic review of animal models for Staphylococcus aureus osteomyelitis

In vivo efficacy of a silicone‒cationic steroid antimicrobial coating to prevent implant-related infection

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