Experimental Model of Arthritis Induced by Paracoccidioides brasiliensis in Rats

Loth, Eduardo Alexandre; Biazin, Samia Khalil; Paula, Claudete Rodrigues; Simão, Rita de Cássia Garcia; Franco, Marcello; Puccia, Rosana; Gandra, Rinaldo Ferreira

doi:10.1007/s11046-012-9537-8

Cited by 4 publications

(5 citation statements)

References 15 publications

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“…The experimental model of infectious arthritis induced by P. brasiliensis is characterized by the development of an intense infectious response, plus an inflammatory response, represented by the formation of joint edema and an increase in leukocytes number, recruited at the lesion area, corroborating with Loth et al (2012) research. This response reached the highest peak on the 14 th day after induction, and it remained stable on the 21 st day.…”

Section: Discussionsupporting

confidence: 77%

“…P. brasiliensis has put forward an edema formation at the infection area by progressively increasing vascular permeability until the 15 th day of induction, and there was some stabilization of the joint diameter after this period (Loth et al 2012). Loth et al (2014) deployed the model of infectious arthritis induced by P. brasiliensis in rats and verified, by radiological analysis, an increase in joint destruction.…”

Section: Discussionmentioning

confidence: 98%

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Effect of anethole and anethole+itraconazole assiciantion on experimental arthritis induced by Paracoccidioides brasiliensis

Wisniewski,

Rocha,

Loth

et al. 2024

RSD

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In this study, the effect of treatment with anethole (AN) and the combination anethole+itraconazole (AN+IT) compared to IT, on infectious arthritis induced by Paracoccidiodes brasiliensis, was evaluated. The animals were treated for 14 days at doses of anethole (AN - 62.5, 125 and 250 mg/kg), itraconazole (IT - 12.5, 25 and 50 mg/kg) and the combination of anethole+itraconazole (AN+ IT - 62.5 and 12.5 mg/kg). The parameters evaluated were: the development of knee edema, the number of leukocytes recruited into the joint cavity, the body weight of the animals, the walking capacity, the plasmatic concentration of nitric oxide, the concentration of TNF in the knee joint exudate, the production of anti-Pb antibodies and the activity of plasma transaminases (AST and ALT). Histological changes in the right knee joint of the hind paw were evaluated using Hematoxylin-eosin and Grocott staining. The results showed that treatment with monotherapies and combinations reduced knee joint edema, the number of leukocytes recruited into the synovial cavity and improved the animals' gait. The concentration of plasma NO, tissue TNF and anti-Pb were reduced by treatment with IT at all doses tested and with the AN+IT combination. Treatment with AN only reduced the plasma concentration of NO and tissue TNF at a high dose. Altogether, the data showed that treatments with IT monotherapy and the combination of AN + IT showed a similar inhibitory effect on the development of arthritis.

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Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 98%

Effect of anethole and anethole+itraconazole assiciantion on experimental arthritis induced by Paracoccidioides brasiliensis

Wisniewski,

Rocha,

Loth

et al. 2024

RSD

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“…Os animais do GCA receberam infusão intra-articular de 50 μl de solução salina tampão fosfatada estéril, no joelho direito da pata posterior e serviu como controle absoluto do estudo. Os animais dos demais grupos foram submetidos ao desenvolvimento experimental de artrite de acordo com modelo descrito por Loth et al [16] que mimetiza a paracoccidioidomicose articular em humanos, através da injeção de 50 μl de 3/9 isolado de P. brasiliensis da linhagem Pb18 viáveis com concentração 10 5 . Todos os animais, previamente aos experimentos, foram anestesiados com 50mg/kg de cetamina e 5mg/kg de midazolan por via intraperitoneal.…”

Section: Métodosunclassified

Terapia fotodinâmica na artrite experimental induzida por Paracoccidioides brasiliensis

Genske

Loth

Cecatto

et al. 2019

Sci Med

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AIMS: To evaluate the effectiveness of photodynamic therapy with Brilliant Blue G in the treatment of an experimental model of arthritis by Paracoccidioides brasiliensis (P. brasiliensis). METHODS: After the induction of experimental arthritis with isolated from P. brasiliensis of lineage Pb18 in the knees of Wistar rats, the animals were divided into groups and submitted to photodynamic therapy with intra-articular Brilliant Blue G photosensitizer and laser therapy only, without Brilliant Blue G. All groups received their respective treatments from the seventh to the 11th day. For edema analysis, the knee lateral-lateral diameter of each animal was measured daily and after the treatment period the animals were sacrificed for experimental knee dissection and blood collection for analysis by ELISA, in order to quantify levels of anti-P. brasiliensis antibodies. RESULTS: The results showed that the application of photodynamic therapy was able to prevent the formation of edema when compared to the control (p>0.005), as well as the production of anti-Gp-43 antibodies from P. brasiliensis (p=0.001). In the anatomopathological examination it was possible to observe a higher degree of synovitis and a greater presence of granulomas with the fungus inside the group that did not receive treatment when compared to the groups that received the photodynamic therapy. CONCLUSIONS: Photodynamic therapy was effective in attenuating the experimental arthritis induced by P. brasiliensis in the proposed joint model.

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“…During prior development of the experimental model employed in this study, intense infl ammatory signs and focal necrosis occurred at day 45 of exposure 7 . In the current study, lytic necrosis was only observed to be present by day 90.…”

Section: Figurementioning

confidence: 99%

“…Induction of experimental arthritis took place as described by Loth et al 7 . The animals were anesthetized with ketamine and xylazine, intraperitoneally (50 mg/kg and 10 mg/kg body weight).…”

mentioning

confidence: 99%

Natural history of experimental arthritis induced by Paracoccidioides brasiliensis in wistar rats

Loth

Cecatto

Genske

et al. 2019

Rev. Soc. Bras. Med. Trop.

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Introduction: Paracoccidioidomycosis (PCM) is the most prevalent systemic mycosis in Latin America. This study aimed to evaluate the natural history of Paracoccidioides brasiliensis-induced experimental arthritis of the knee joints in Wistar rats. Methods: Rats were randomly allocated to either an absolute control group, or 15-day, 45-day, or 90-day experimental (fungusinoculated) groups. Results: Experimental groups developed classic signs of articular PCM. Titers of anti-gp43 were observed to increase during the interval from 15 to 45 days post-inoculation. Conclusions: Articular arthritic lesions were induced and progressed during the study period in all experimental groups.

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Experimental Model of Arthritis Induced by Paracoccidioides brasiliensis in Rats

Cited by 4 publications

References 15 publications

Effect of anethole and anethole+itraconazole assiciantion on experimental arthritis induced by Paracoccidioides brasiliensis

Effect of anethole and anethole+itraconazole assiciantion on experimental arthritis induced by Paracoccidioides brasiliensis

Terapia fotodinâmica na artrite experimental induzida por Paracoccidioides brasiliensis

Natural history of experimental arthritis induced by Paracoccidioides brasiliensis in wistar rats

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