Abstract-According to the intracellular electromanipulation model, bacteria can be killed at as high as 10 6 V/m electrical fields on microwave band. We designed and constructed a modified microwave coaxial cavity resonator for liquid sterilization. The cavity could concentrate the field on a very small area, and the liquid can pass it in milliseconds. The bacteria can be killed by the very high field, but with a slight temperature increase. The designed resonator is simulated and analyzed by electromagnetic simulation code. The results indicated that when the input power reached 100 W, the electric field on the area of liquid can reach 10 6 V/m. Preliminary experimental results indicated that when the input power was 100 W, the bactericidal rate was > 90%, and the temperature of the liquid only increased 8.6 • C.T M