Experimental meningococcal infection in neonatal mice: differences in virulence between strains isolated from human cases and carriers

Salit, Irving E.; Tomalty, Lewis

doi:10.1139/m84-162

Cited by 11 publications

(7 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies by several authors [25,26] showed a clear relationship between growth in iron-restricted conditions and virulence in N. meningitidis strains and, as proteins which axe expressed under such conditions have been implicated as functional components of the iron-uptake systems of a number of bacteria [3], it can be expected that also in IV, meningitidis they are involved in these virulence processes.…”

Section: Meningitidismentioning

confidence: 99%

Analysis of the expression of outer-membrane proteins in Neisseria meningitidis in iron-replete and iron-deficient media

FerreirÃ3s¹,

Criado²,

Rao³

et al. 1990

FEMS Microbiology Letters

View full text Add to dashboard Cite

A total of 103 Neisseria strains, including 42 carrier and 40 invasive N. meningitidis and 21 commensal species were studied. Outer‐membrane proteins from carrier and invasive N. meningitidis showed similar molecular mass distributions (except in the range from 78 to 82 kDa in which 90% of the invasive but only 47.6% of the carrier strains expressed proteins), many strains showin neither class II (41 kDa) nor class III (38 kDa) proteins. When grown in iron‐restricted conditions proteins were induced mainly in the ranges from 62 to 92 kDa with no significant differences between groups. Commensal species, both in normal and in iron‐restricted conditions, showed outer‐membrane protein patterns different from those of N. meningitidis in several molecular mass ranges. Cluster analysis based on expression of principal outer‐membrane proteins allowed the differentiation of commensal species and N. meningitidis, although not that of the invasive and carrier groups within the latter.

show abstract

Section: Meningitidismentioning

confidence: 99%

Analysis of the expression of outer-membrane proteins in Neisseria meningitidis in iron-replete and iron-deficient media

FerreirÃ3s¹,

Criado²,

Rao³

et al. 1990

FEMS Microbiology Letters

View full text Add to dashboard Cite

show abstract

“…i.n. models have mainly used neonatal mice (28,38) to analyze disease pathogenesis and virulence of meningococcal isolates (29,37). Interestingly, meningococcemia was also induced by the i.n.…”

mentioning

confidence: 99%

The Meningococcal ABC-Type l -Glutamate Transporter GltT Is Necessary for the Development of Experimental Meningitis in Mice

Colicchio

Ricci²,

Lamberti

et al. 2009

Infect Immun

View full text Add to dashboard Cite

Experimental animal models of bacterial meningitis are useful to study the host-pathogen interactions occurring at the cerebral level and to analyze the pathogenetic mechanisms behind this life-threatening disease. In this study, we have developed a mouse model of meningococcal meningitis based on the intracisternal inoculation of bacteria. Experiments were performed with mouse-passaged serogroup C Neisseria meningitidis. Survival and clinical parameters of infected mice and microbiological and histological analysis of the brain demonstrated the establishment of meningitis with features comparable to those of the disease in humans. When using low bacterial inocula, meningococcal replication in the brain was very efficient, with a 1,000-fold increase of viable counts in 18 h. Meningococci were also found in the blood, spleens, and livers of infected mice, and bacterial loads in different organs were dependent on the infectious dose. As glutamate uptake from the host has been implicated in meningococcal virulence, mice were infected intracisternally with an isogenic strain deficient in the ABC-type L-glutamate transporter GltT. Noticeably, the mutant was attenuated in virulence in mixed infections, indicating that wild-type bacteria outcompeted the GltT-deficient meningococci. The data show that the GltT transporter plays a role in meningitis and concomitant systemic infection, suggesting that meningococci may use L-glutamate as a nutrient source and as a precursor to synthesize the antioxidant glutathione.

show abstract

“…We have described a neonatal mouse model for meningococcal disease in which the intranasal inoculation of bacteria is followed by a phase of nasopharyngeal colonization (25,26). Virulent and avirulent strains attach to and colonize the murine nasopharynx to the same degree, but it is mainly those strains which are virulent for humans which invade through the nasal mucosa and cause bacteremia.…”

Section: Discussionmentioning

confidence: 99%

“…Strains of N. meningitidis isolated from blood or spinal fluid were designated "case strains," whereas those derived from the pharynxes of asymptomatic persons were designated "carrier strains." The strains which were used in this study and previous studies (25,26) were characterized as to capsular serogroup and serotype (serogroup/serotype). Disease-associated strains were B16B6 (B/2a), M986 (B/2a,7), 3006 (B/2b), BB80 (B/2), M1011 (B/2,10), BB106 (C/2), BB138 (C/2), BB155 (C/2), and VM63 (nongroupable).…”

Section: Methodsmentioning

confidence: 99%

“…In addition to protecting against clinical illness, appropriate vaccines should also have the ability to prevent meningococcal carriage in the nasopharynx and hence eliminate the major reservoir of infection. We have previously studied experimental meningococcal infection in rats, guinea pigs, and mice (25,26). The most appropriate experimental model involved using neonatal mice who developed nasopharyngeal carriage followed by bacteremia, meningitis, and death.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Experimental meningococcal infection in mice: a model for mucosal invasion

Salit¹,

Tomalty²

1986

Infect Immun

View full text Add to dashboard Cite

A nmore complete understanding of meningococcal disease has been hampered by the lack of an appropriate animal model. Previous models have utilized injections of meningococci, which precludes the study of nasopharyngeal colonization and invasion. We have developed a model for meningococcal disease in which litters of 5-day-old mice are challenged intranasally with 107 viable meningococci. Bacteremia is monitored by jugular venous blood cultures, and cerebrospinal fluid is sanlpled by cisternal punctures. Human diseaseassociated and carrier strains were compared; nasopharyngeal colonization was similar for these bacteria, but the case-associated strains were much more frequently invasive and caused bacteremia. Twenty-one percent of bacteremic animals had meningitis. There was an age-related susceptibility to infection which correlated inversely with the levels of serum complement. Prein'ection of iron dextran increased the number of animals which were bacteremic, the concentration of bacteria in blood, and nasopharyngeal colonization. Noncapsular variants of virulent meningococci did not colonize nasopharyngeal tissue in vivo, and they were not invasive. This neonatal mouse model mimics meningococcal disease as seen in humans and may be useful in studying the initial events in the pathogenesis of meningococcal disease.

show abstract

Experimental meningococcal infection in neonatal mice: differences in virulence between strains isolated from human cases and carriers

Cited by 11 publications

References 0 publications

Analysis of the expression of outer-membrane proteins in Neisseria meningitidis in iron-replete and iron-deficient media

Analysis of the expression of outer-membrane proteins in Neisseria meningitidis in iron-replete and iron-deficient media

The Meningococcal ABC-Type l -Glutamate Transporter GltT Is Necessary for the Development of Experimental Meningitis in Mice

Experimental meningococcal infection in mice: a model for mucosal invasion

Contact Info

Product

Resources

About