Experimental Leptospirosis in Pregnant Sows

Fennestad, K. L.; Borg-Petersen, C.

doi:10.1093/infdis/116.1.57

Cited by 14 publications

(13 citation statements)

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“…Removal of leptospiras from the kidney tubules soon after commencement of leptospiruria was associated with a much more rapid decrease in serum agglutinating antibody titre than that observed in the untreated control animals, or in an animal treated at a later stage in the disease. This supports the hypothesis (Fennestad 1963) that leptospiras in the kidney tubules continue to stimulate antibody production.…”

Section: Discussionsupporting

confidence: 88%

STREPTOMYCIN TREATMENT OF BOVINE CARRIERS OF LEPTOSPIRA PAMONA

Doherty¹

1967

Aust Veterinary J

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Section: Discussionsupporting

confidence: 88%

STREPTOMYCIN TREATMENT OF BOVINE CARRIERS OF LEPTOSPIRA PAMONA

Doherty¹

1967

Aust Veterinary J

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“…Nervig, Ellinghausen and Cardella (1978) infected calves with serovar szwajizak, another member of the Hebdomadis serogroup, and reported that all had overt clinical signs and leptospiraemia, but only 2 of 3 calves developed detectable MA antibody titres to the infecting serovar. Fennestad (1963) inoculated 6 young calves subcutaneously with sejroe, another closely related serovar, and produced mild disease with fever in 5 3-week-old calves and severe disease in a 2-day-old calf. Leptospiruria, moderate serological responses and interstitial nephritis were reported.…”

Section: Discussionmentioning

confidence: 99%

EXPERIMENTAL INFECTION OF CALVES AND SHEEP WITH LEPTOSPIRA INTERROGANS SEROVAR BALCANICA

Durfee¹,

Presidente²

1979

Aust J Exp Biol Med

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Summary Two of four calves inoculated with Leptospira interrogans serovar balcanica developed low microscopic agglutinating (MA) titres to serovar hardjo. A third calf had an MA titre of 1:1024 by day 19 post‐inoculation (PI). Transient leptospiruria was recorded in one calf on days 12 and 13 PI. An in‐contact calf did not seroconvert. None of the calves had fever or other clinical signs of disease. Four ewes inoculated with balcanica developed MA titres to hardjo by day 13 PI, and a transient leptospiruria between days 14 and 25 PI. None of the ewes showed any evidence of clinical disease and three of them delivered healthy lambs 22 to 64 days PI. One ewe had mild lesions of focal interstitial nephritis.

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“…DNA sample amplification was done in 500 µL microtubes, with a 50 µL final volume. The reaction mixture consisted of 18.7 µL ultrapure water, 5.0 µL 10x reaction buffer (500 mM KCl; 15 mM MgCl2; 100 mM tris-HCl, pH 9.0), 8 The amplification cycle used was the one recommended by Mérien et al (19), added with an initial step at 94ºC for 5 minutes. Visualization of the amplified product (330 base pairs) was done by electrophoresis in a 2.0% (w/v) agarose gel stained with ethidium bromide, using TBE 0.5 X (0.04 M tris-acetate and 0.001M EDTA, pH 8.0) as running buffer.…”

Section: Polimerase Chain Reaction (Pcr)mentioning

confidence: 99%

“…Guida et al The pathogenesis of the reproductive disease is poorly understood, but some authors believe that transplacental infection, occurring during a very limited period of maternal leptospiremia, is the sole cause (4). The isolation of leptospires from aborted fetuses, stillbirths and weak piglets was obtained in several occasions (5,8,27), however, the bacteria was never detected in apparently healthy piglets born from apparently healthy infected dams. Although organisms belonging to the Canicola serogroup have been recovered from swine in several countries (4), little is known on the epidemiology of serovar Canicola infection in pigs.…”

Section: Introductionmentioning

confidence: 99%

Detection of leptospires in clinically healthy piglets born from sows experimentally infected with Leptospira interrogans serovar Canicola

Soto¹,

Azevêdo²,

Morais³

et al. 2006

Braz. J. Microbiol.

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Leptospirosis is an important zoonosis that causes reproductive disorders in swine. The isolation of leptospires from aborted fetuses, stillbirths and weak piglets was obtained in several occasions, however, the bacteria was never isolated from apparently healthy piglets born from apparently healthy infected dams. Six sows of the Landrace breed with a known date of service and pregnancy confirmed by ultrasonography were infected intravenously with 5 ml of Leptospira interrogans serovar Canicola inoculum between 76 and 90 days of gestation, and one week after farrowing, at least one piglet per sow was euthanized and samples of liver, kidneys, lungs, heart, spleen and gastric content were taken and examined by PCR. Reproductive disorders or any clinical sign of infection were not observed in the inoculated sows. The piglets born from these animals presented no clinical signs or macroscopic lesions that could be attributed to leptospirosis. All inoculated sows presented anti-leptospires antibodies by microscopic serum-agglutination test (MAT) in the postinoculation serum samples and leptospires were not found in the urine as well as were not detected by PCR applied in this material, however, PCR accomplished in kidneys and liver from a euthanized sow presented positive results. Of the total of 12 euthanized piglets, 10 (83.3%) presented positive results by PCR in at least one of the kidney, liver, heart, spleen, lung and gastric content samples. The present study reports the vertical transmission of the infection and the detection of leptospires in clinically healthy piglets born from experimentally infected sows, which is important of the epidemiological point of view as the maintenance of clinically healthy infected animals may allow the persistence of the bacteria in the herd, exposing other animals to the risk of infection.

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Experimental Leptospirosis in Pregnant Sows

Cited by 14 publications

References 0 publications

STREPTOMYCIN TREATMENT OF BOVINE CARRIERS OF LEPTOSPIRA PAMONA

STREPTOMYCIN TREATMENT OF BOVINE CARRIERS OF LEPTOSPIRA PAMONA

EXPERIMENTAL INFECTION OF CALVES AND SHEEP WITH LEPTOSPIRA INTERROGANS SEROVAR BALCANICA

Detection of leptospires in clinically healthy piglets born from sows experimentally infected with Leptospira interrogans serovar Canicola

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