Experimental intoxication with fruit and purified toxins of buckthorn (Karwinskia humboldtiana)

Bermúdez, Martha; Gonzalez-Spencer, D.; Guerrero, Martha Inírida; Waksman, Noemí; Piñeyro, A.

doi:10.1016/0041-0101(86)90135-2

Cited by 46 publications

(38 citation statements)

References 2 publications

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“…These manifestations were present in the paresis group at 48 days, were more evident at 58 days in the paralysis group, and almost all of the symptoms resolved in the recovery group, which only presented piloerection. These results agree with other studies regarding the clinical signs in humans and animals intoxicated with Kh fruit (Padron-Puyou, 1951;Padrón and Velázquez, 1956;Bermúdez et al, 1986;Salazar et al, 2006, García-Juárez et al, 2012.…”

Section: Discussionsupporting

confidence: 83%

“…These signs agree with the findings reported by Bermúdez et al, (1986) who studied the effect of T-514, one of the toxins isolated from Kh fruit on liver.…”

Section: Discussionsupporting

confidence: 82%

“…Another important point is that even though experimental studies of acute intoxication in different animal species with isolated toxins or Kh fruit have reported similar alterations in liver to those observed in the present investigation (Bermúdez et al, 1986;1992). These studies do not report the progression and recovery of the histopathological damage in the liver observed in the present investigation.…”

Section: Discussionmentioning

confidence: 47%

“…Since numerous reports of accidental intoxication in humans (Arellano et al, 1994;Bermúdez et al, 1995;Ocampo et al, 2007) or experimental intoxication in animals with Kh fruit (Escobar and Nieto, 1965;Charlton and Pierce, 1970;Muñóz and Chávez, 1979;Muñóz et al, 1983;Bermúdez et al, 1986;Bermúdez et al, 1992;Salazar et al, 2006, García-Juárez et al, 2012 were focused on the alterations of peripheral nerve, it was considered for a long time that Kh affects exclusively peripheral nerve system (PNS). However, this study is the first to describe the histopathological alterations in the liver caused by Kh fruit in an experimental model of peripheral neuropathy.…”

Section: Discussionmentioning

confidence: 99%

“…Only there are two in vitro studies in hepatocytes of rat treated with Kh fruit (Wheeler et al, 1971), or with T-514 (GarzaOcañas et al, 2003) that reported mitochondrial alterations and increase of toxic oxygen radicals. In vivo experimental studies of acute toxicity with purified toxin T-514 administered to different species have reported atelectasis and emphysema, along with infiltration of polymorphonuclear cells in alveolar septa, rupture of capillaries and bleeding (Bermúdez et al, 1986;Sepúlveda et al, 1992). In liver, congestion and massive necrosis were reported, especially with T-514 (Bermudez et al, 1986).…”

Section: Introductionmentioning

confidence: 99%

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Liver damage in an experimental model of peripheral neuropathy induced by Karwinskia humboldtiana (Buckthorn) fruit: Histopathological evidence

García-Garza¹,

Salazar-Leal²,

Romero-DÃaz³

et al. 2016

J. Med. Plants Res.

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Karwinskia humboldtiana (Kh) is a poisonous shrub that causes acute or chronic intoxication in animals and humans. In chronic intoxication, the main sign is the presence of paralysis. Previously we reported a model of intoxication with Kh fruit that reproduces paralysis and clinical phases in humans when they do not die; however, there are no studies that describe damage to liver in this model. The objective was to evaluate histopathological alterations in liver during chronic intoxication. Wistar rats (n=25) were divided into five groups (n= 5). Four groups were intoxicated with Kh and one received only water as a control group. Animals were euthanized at different times during paralysis evolution. Samples of liver were obtained, and processed either for light or electron microscopy evaluation. Histological, histochemistry and immunohistochemistry analyses were performed, including a morphometric analysis. Results demonstrated necrotic areas, vascular congestion and vacuoles in the cytoplasm of hepatic cells that increased during intoxication and decreased in the recovery stage. These findings were confirmed at electron microscopy level. Morphometric analysis demonstrated statistically significant difference in occurrence of necrotic cells and PCNA positive cells between control and intoxicated groups. This was not observed with TUNEL staining. These findings demonstrate that during Kh intoxication there is a severe damage in liver that is reversible. Thus, it could be suggested that Kh causes a systemic intoxication not only damage to peripheral nervous system.

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Section: Discussionsupporting

confidence: 83%

“…These signs agree with the findings reported by Bermúdez et al, (1986) who studied the effect of T-514, one of the toxins isolated from Kh fruit on liver.…”

Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 47%